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A insecticide and its application

A technology of insecticide and mycocin, which is applied in the field of plant protection and pesticides, can solve the problems of difficult to avoid environmental pollution, lack of control effect of resistant diamondback moth and water spider, etc., to overcome drug resistance, use safe and easy to operate Effect

Inactive Publication Date: 2004-12-29
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

General insecticidal chemicals are easy to make pests resistant to the pesticides, and it is difficult to avoid environmental pollution. At the same time, they lack the control effect against resistant diamondback moths and water spiders

Method used

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  • A insecticide and its application
  • A insecticide and its application
  • A insecticide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The insecticide Tianshanlin was prepared by culturing the strain GAAS7310 in a fermentation medium. Thaw 1 ml of frozen GAAS7310 nutrient medium, inoculate into 100 ml of nutrient medium in a 500 ml Erlenmeyer flask, and grow for 48 hours at 28° C. and shaking at 250 rpm.

[0025] Take 3500ml of the mature first-stage vegetative culture to inoculate the secondary nutrient culture of 35L of nutrient medium in a 50L stirred fermenter, cultivate it at 28°C and 300rpm for 48 hours, and inoculate the mature strain into 500L In the agitated fermenter of , the fermentation culture was maintained at 28° C. and 300 rpm for 4 days. The mature fermented product can be extracted with a suitable solvent and separated by chromatography to obtain the metabolite—the insecticide Tianshanlin.

[0026] Table 2 Nutrient medium composition

[0027] Ingredient name Content (%)

[0028] Glucose 2

[0029] starch 1

[0030] rice flour 1

[0031] yeast paste 1

[0032] B...

Embodiment 2

[0038] The present invention tracks the production of the insecticide asanthanin during fermentation by testing an extract of the fermentation broth, eg, by analyzing the fermentation broth extract by a bioassay.

[0039] After the fermentation is completed, the mycelium is separated from the 500L fermented product by plate and frame filter press, and the mycelium is leached with 1:2 acetone solution for 10 hours, the leaching solution is concentrated to remove acetone, and then extracted with ethyl acetate For the effective substance, the ethyl acetate extract is concentrated under reduced pressure to obtain an oily substance, the extracted concentrated solution is mixed with silica gel and passed through a column, and the silica gel column chromatography and concentration are performed with ethyl acetate and petroleum ether to obtain a pure oily substance.

[0040] The compound is an oily liquid, from 1 H and 13 CNMR shows that the molecule is a compound with a long chain. ...

Embodiment 6

[0048] The insecticide Tianshanmycin described in the present invention is suitable for controlling insects, mites and ticks, including the use of inhibitory doses of insects, mites and ticks at places where insects, mites and ticks are used. The locus of insects, mites, and ticks means an environment in which larvae, adults, and eggs of insects, mites, and ticks include air, food, and objects with which they come into contact. For example, phytophagous insects or mites can be controlled by applying the active compounds to plant parts, especially leaves, which the insects and mites feed on or parasitize. The effect of controlling insects by using the insecticide Tianshanmycin of the present invention is related to the frequency of use and the method of use, and is also related to the species of insects to be controlled.

[0049] When the pak choy is potted and grows to 5 leaves, 50-60 young resistant Plutella xylostella larvae are inserted into each pot. After the activity of ...

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Abstract

The present invention is Tianshan bacterin as one kind of pesticide and its application. Tianshan bacterin is suitable for preventing and killing mite, tick and other pests and has excellent preventing and killing effect on Coleoptera pests, weevil, Dipters pest, Anoplura pest, Isoptera pest, hymenopteran ant, mite and animal parasite. The present invention has simple preparation process, less consumption, less resistance, high safety and no environmental pollution.

Description

technical field [0001] The invention relates to the technical fields of plant protection and pesticides, in particular to an insecticide tianshanmycin and its application. Background technique [0002] Insecticidal antibiotics are one of the important biopesticides. It is a natural biopesticide with the characteristics of low toxicity to humans and animals and rapid degradation. Therefore, it has become one of the hot spots of research and development at home and abroad in recent years. General insecticidal chemicals are easy to make pests resistant to the pesticides, and it is difficult to avoid environmental pollution. At the same time, they lack the control effect against resistant diamondback moths and water spiders. Contents of the invention [0003] The object of the present invention is to provide a kind of insecticide Tianshanmycin and its application in order to solve the shortcomings in the above-mentioned prior ...

Claims

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Application Information

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IPC IPC(8): A01N63/02C12N1/20
Inventor 林壁润谢双大江学斌周小毛冼海辉沈会芳杨丽梅陈光英林永成
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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