Microorganism oil production method

A technology for microbial oil recovery and compound microorganisms, which is applied in the fields of fluid production, earth-moving drilling, wellbore/well components, etc. It can solve the problem of difficulty in examining the effect of microbial oil recovery technology, long reaction time between microorganisms and nutrient solution, and complicated pipeline network transformation and construction. and other problems, to achieve the effect of simple construction, low cost and increased mobility

Inactive Publication Date: 2004-12-29
TIANJIN GUARD T & D +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, from the published literature, the various microbial oil recovery methods so far are only limited to the single action of hydrocarbon-decomposing bacteria.
US 4,475,590 proposes a method of injecting a large amount of nutrient solution from the water injection well into the reservoir, the purpose is to stimulate the growth and metabolism of microorganisms inherent in the reservoir to improve the recovery; US 5,163,510 discloses the injection of aerobic bacteria from the production well and then inject The method of oxygen, its purpose is to allow the growth and metabolism of microorganisms better, but the construction is complicated, the cost is too high, and it is not safe; US 4,450,908 proposes the method of injecting polyacrylamide from the water injection well and then injecting microorganisms; US 4,905,761 is A patent on injecting microorganisms and nutrient solution into formations from water injection wells; CN 1038327 proposes to enrich microorganisms from the water produced from each oil well, and then inject the nutrient solution and microorganisms back into the oil reservoir from the original well after laboratory experiments. The disadvantage is that hydrocarbon-decomposing bacteria need to be enriched well by well for experimental evaluation, the reaction time between microorganisms and nutrient solution is long, and the well shut-in time is required to be as long as 20 days; CN 1164607A discloses a method of circulating microbial flooding and recovery
Its technology consists of two aspects. On the one hand, microorganisms are injected into production wells or water injection wells. On the other hand, microorganisms that survive and multiply in the oil layer are extracted from production wells, separated from oil and water, and then the sewage containing bacteria is injecte

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0015] Example 1

[0016] Bacillus licheniformis (Bacillus licheniformis) X 3 The fermentation broth and the hydrocarbon-decomposing mixed bacteria culture broth is made into a composite microbial preparation at a ratio of 1:1.5-9, in which Bacillus licheniformis X 3 The content of lipopeptide surfactant in the fermentation broth is 1~4g / L, and the hydrocarbon-removing mixed bacteria contains Bacillus sp. N 2 , Pseudomonas sp. N 5 And Acinetobacter (Acinetobacter sp.) N 13 , In the culture medium of the mixed bacteria decomposing hydrocarbon, Bacillus sp. N 2 , Pseudomonas sp. N 5 And Acinetobacter (Acinetobacter sp.) N 13 The volume ratio of the culture broth is 4:3:3, the pH of the culture broth of the hydrocarbon-decomposing mixed bacteria is 5.0~6.0, the surface tension is 42~45mN / m, and the bacterial concentration is 10 8 ~10 9 Pcs / ml; dilute the stock solution of the composite microbial preparation by 2-10 times with water to prepare a diluent of the composite microbial prep...

Example Embodiment

[0017] Example 2

[0018] Core simulation flooding comparison test, the steps are as follows:

[0019] a. Prepare 4 steel pipes with an inner diameter of 2.5cm and a length of 30cm, and fill them with 80-160 mesh quartz sand, with both ends closed, leaving a pipe-shaped inlet and outlet;

[0020] b. Measure the permeability of 0.273~0.288μm 3 , Pumping a vacuum to saturate formation water, measuring porosity of 37.2~39.5%, pore volume of 30.50~31.80mL, establishing irreducible water through oil injection and flooding water, measuring the original oil saturation of 84.3~88.4%, the oil is taken from Dagang Oilfield, The oil contains 30% wax and 25% gum asphalt;

[0021] c. Test design: After each core is saturated with oil, the formation water is used to drive the water content of the produced fluid to more than 98%, 1 # Core as a control, 2 # The core is injected with a pore volume of 0.2 pore volume of a mixed nutrient-containing hydrocarbon decomposing bacteria culture solution c...

Example Embodiment

[0031] Example 3

[0032] The steps of "microbial flooding" with the composite microbial preparation of the present invention are as follows:

[0033] a. Select a water injection and oil production block suitable for microbial flooding. The conditions for the water injection and oil production block selected in this example are: the buried depth of the reservoir is 500-3000m, the temperature is less than 70°C, the salinity is less than 100000 mg / L, and the penetration Rate>0.12μm 2 , Residual oil saturation> 25%, oil wax content> 30%, gum content> 25%, produced fluid water content ≥ 80%, single well daily oil production ≤ 5 tons;

[0034] b. Suspend water injection and flood oil, use the existing water injection pipeline to slightly modify, and inject the composite microbial preparation diluent prepared according to Example 1 with 5-10 times of the composite microbial preparation stock solution with water, and inject it into the reservoir. Estimate the pore volume inside the reser...

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Abstract

The microbial oil extracting method of the present invention is to inject the composite microbial preparation comprising fermented Bacillus liceni formis X3 liquid and mixed hydrocarbon utilizing bacteria culture solution in the ratio of 1 to 1.5-9 into petroleum deposit via water injection well or to the zone near the well via oil well. The fermented Bacillus liceni formis X3 liquid has lipopeptide surfactant content of 1-4 g/L. The present invention is one kind of microbial oil extracting method of microbial oil displacement or single well gorge and disgorge and has simple construction process, short well closing period, low cost and no pollution to stratum and environment.

Description

Technical field [0001] The technical solution claimed by the present invention relates to a method for improving oil extraction by using bacterial activities, specifically a method for microbial oil extraction. Background technique [0002] Hydrocarbon decomposing bacteria and nutrient solution are injected into the reservoir through the water injection well (the process term is called "microbial flooding") or squeezed from the oil well to the near-well zone (the process term is called "single well huff and puff"). The degradation of crude oil and the effect of its metabolites on crude oil emulsify crude oil to reduce viscosity and increase fluidity. It is a method that has been applied to increase oil production or recovery. However, from the published literature, the various microbial oil recovery methods so far are only limited to the role of hydrocarbon decomposing bacteria alone. US 4,475,590 proposes a method of injecting a large amount of nutrient solution from a water inj...

Claims

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Application Information

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IPC IPC(8): E21B43/22
Inventor 刘如林梁凤来马建芳顾晓波李伯平
Owner TIANJIN GUARD T & D
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