Hypocrellin water-soluble nanogranule and its uses

A kind of hypocretin and water-soluble technology, which is applied in the field of water-soluble nanoparticles and intravenous injections as photodynamic drugs, and achieves the effects of simple preparation method, good targeting and biodegradability, and easy operation

Inactive Publication Date: 2005-01-19
INST OF CHEM CHINESE ACAD OF SCI +1
View PDF2 Cites 19 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a new type of water-soluble hypocrellin nanoparticles to meet the dual requirements of smooth transmission of photodynamic drugs in the blood and adsorption on the cell surface, to develop a new dosage form and provide the preparation of the above-mentioned water-soluble nanoparticles. The new method solves the practical problem of hypocretin phot

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hypocrellin water-soluble nanogranule and its uses
  • Hypocrellin water-soluble nanogranule and its uses
  • Hypocrellin water-soluble nanogranule and its uses

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0024] Example 1

[0025] Add 5ml of 1%~5% (weight volume percentage) medicinal gelatin solution, add 0.5%~3.0% (weight volume percentage) of Tween-80, heat up to 30℃~60℃, slowly add 1ml bamboo red under vigorous stirring DMSO solution of Bacteriobacterin (HB) (3mg / ml, the solvent used to dissolve Hypocrellin is DMSO), add 3ml of absolute ethanol, and then sonicate at 5℃~16℃ at low temperature After 1 to 10 minutes, the solution starts to show strong scattered light. At this time, stir again and heat up to 30℃~60℃, the scattered light disappears, so it can be cycled many times, and then add 0.4ml of curing agent glutaraldehyde to cure 20 ~40 minutes, dialysis to remove ethanol and DMSO, freeze-drying to obtain Hypocrellin B gelatin nanoparticles, and refrigerate in a refrigerator at 4°C.

Example Embodiment

[0026] Example 2

[0027] Add 5ml of 0.02%~0.5% (weight volume percentage) sodium alginate aqueous solution, slowly adding 0.5ml of Hypocrellin (HB) in DMSO solution (3mg / ml) under vigorous stirring. The solvent used for dissolving Hypocrellin is selected Dimethyl sulfoxide (DMSO), add 0.22ml of a calcium chloride aqueous solution with a concentration of 0.1 to 0.5% (weight / volume ratio, W / V), continue to stir for 20 to 40 minutes, then ultrasound for 1 to 10 minutes, and finally remove by dialysis Hypocrellin B alginate nanoparticles were obtained from DMSO small molecule organic solvent, and kept in refrigerator at 4°C.

Example Embodiment

[0028] Example 3

[0029] Chitosan is dissolved in 1% acetic acid solution and prepared into a solution with a concentration of 0.02~0.5% (weight volume percentage). Pipette 5.0ml, and under vigorous stirring, add 0.5ml Hypocrellin (HB) DMSO solution (3mg / ml, the solvent used to dissolve Hypocrellin is DMSO), and then add 2ml of 0.1-0.5% sodium polyphosphate solution, continue to stir for 20-30 minutes, then ultrasound 1 to 10 minutes, finally dialysis to remove the DMSO small molecule organic solvent to obtain Hypocrellin B chitosan nanoparticles, and refrigerate in a refrigerator at 4°C.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention relates to a Hypocrellin water-soluble nanogranule and its uses characterized in that, native protein, polysaccharide macromolecule and biodegradable high molecular polymer can be used as packing material, the liposoluble bamboo bacterioerythrin medicament is packed for preparing ultra-microminiature water-soluble intravenous injection with grain diameter of 20-200nm. These preparations can be used as the intravenous injection for optical dynamic medicament of Hypocrellin.

Description

technical field [0001] The invention relates to a water-soluble nanoparticle made of hypocretin and protein or polysaccharide biological material or macromolecular material and its application as photodynamic drug intravenous injection. Background technique [0002] Hypocrella, including Hypocrella A (HA) and Hypocrella B (HB), is a parasitic fungus from Hypocrella bambusae (B.et Br. ) A class of natural phytochrome extracted from Sacc.). It has the characteristics of ideal photosensitizers such as high singlet oxygen quantum yield, clear structure, good chemical modification, low dark toxicity, and fast metabolism in vivo. It is recognized as the most promising new photodynamic photosensitizer. Folks have used hypocreamin to treat stomach pain, arthritis, and skin diseases; it has been proven that it has potential uses in photodynamic anti-HIV, anti-tumor, and microvascular diseases. [0003] As we all know, the penetration depth of light to human tissue is proportional t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K9/14A61K31/122A61K47/30A61K47/36A61K47/42A61P9/00A61P43/00
Inventor 赵井泉赵宝忠谢杰顾瑛刘凡光
Owner INST OF CHEM CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap