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Hypocrellin water-soluble nanogranule and its uses

A kind of hypocretin and water-soluble technology, which is applied in the field of water-soluble nanoparticles and intravenous injections as photodynamic drugs, and achieves the effects of simple preparation method, good targeting and biodegradability, and easy operation

Inactive Publication Date: 2005-01-19
INST OF CHEM CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a new type of water-soluble hypocrellin nanoparticles to meet the dual requirements of smooth transmission of photodynamic drugs in the blood and adsorption on the cell surface, to develop a new dosage form and provide the preparation of the above-mentioned water-soluble nanoparticles. The new method solves the practical problem of hypocretin photosensitizers as photodynamic drugs; at the same time, the nanoparticles made by the present invention have high stability, good targeting and biodegradability, while maintaining the Mycocin photosensitizers have the characteristics of strong photodynamic function, can be dissolved in water, normal saline, phosphate buffer, and can be conveniently formulated into intravenous injections of hypocrellin drugs

Method used

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  • Hypocrellin water-soluble nanogranule and its uses
  • Hypocrellin water-soluble nanogranule and its uses
  • Hypocrellin water-soluble nanogranule and its uses

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Add 0.5% to 3.0% (weight volume percentage) of Tween-80 to 5ml of 1% to 5% (weight volume percentage) medicinal gelatin solution, heat up to 30°C to 60°C, and slowly add 1ml bamboo red under strong stirring Bacillus B (HB) in DMSO solution (3mg / ml, the solvent used to dissolve hypocrellin is dimethyl sulfoxide DMSO), and add 3ml of absolute ethanol, and then sonicate at a low temperature of 5°C to 16°C After 1 to 10 minutes, the solution begins to show strong scattered light. At this time, stir again and heat up to 30°C to 60°C, and the scattered light disappears. This can be repeated many times, and then add 0.4ml of curing agent glutaraldehyde to cure for 20 minutes. ~40 minutes, dialyzed to remove ethanol and DMSO, freeze-dried to obtain Hypocretin B gelatin nanoparticles, and refrigerated at 4°C in the refrigerator.

Embodiment 2

[0027] Add 0.02% to 0.5% (weight volume percentage) sodium alginate aqueous solution 5ml, slowly add 0.5ml hypocrellin B (HB) DMSO solution (3mg / ml) under strong stirring, the solvent used for dissolving hypocrellin is selected Dimethyl sulfoxide (DMSO), add 0.22ml of calcium chloride aqueous solution with a concentration of 0.1 to 0.5% (weight / volume ratio, W / V), continue to stir for 20 to 40 minutes, then sonicate for 1 to 10 minutes, and finally dialyze to remove DMSO small-molecule organic solvent was used to obtain hypocrellin alginate nanoparticles, which were refrigerated at 4°C in the refrigerator.

Embodiment 3

[0029] Chitosan was dissolved in 1% acetic acid solution, prepared into a solution with a concentration of 0.02 to 0.5% (percentage by weight and volume), pipetted 5.0ml, and added 0.5ml of hypocrellin B (HB) under strong stirring conditions. DMSO solution (3mg / ml, wherein the solvent used to dissolve hypocrellin is dimethyl sulfoxide DMSO), then add 2ml of sodium polyphosphate solution with a concentration of 0.1-0.5%, continue to stir for 20-30 minutes, and then sonicate 1 to 10 minutes, and finally dialyzed to remove the DMSO small molecule organic solvent to obtain hypocrellin chitosan nanoparticles, and refrigerated at 4°C in the refrigerator.

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Abstract

The invention relates to a Hypocrellin water-soluble nanogranule and its uses characterized in that, native protein, polysaccharide macromolecule and biodegradable high molecular polymer can be used as packing material, the liposoluble bamboo bacterioerythrin medicament is packed for preparing ultra-microminiature water-soluble intravenous injection with grain diameter of 20-200nm. These preparations can be used as the intravenous injection for optical dynamic medicament of Hypocrellin.

Description

technical field [0001] The invention relates to a water-soluble nanoparticle made of hypocretin and protein or polysaccharide biological material or macromolecular material and its application as photodynamic drug intravenous injection. Background technique [0002] Hypocrella, including Hypocrella A (HA) and Hypocrella B (HB), is a parasitic fungus from Hypocrella bambusae (B.et Br. ) A class of natural phytochrome extracted from Sacc.). It has the characteristics of ideal photosensitizers such as high singlet oxygen quantum yield, clear structure, good chemical modification, low dark toxicity, and fast metabolism in vivo. It is recognized as the most promising new photodynamic photosensitizer. Folks have used hypocreamin to treat stomach pain, arthritis, and skin diseases; it has been proven that it has potential uses in photodynamic anti-HIV, anti-tumor, and microvascular diseases. [0003] As we all know, the penetration depth of light to human tissue is proportional t...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K31/122A61K47/30A61K47/36A61K47/42A61P9/00A61P43/00
Inventor 赵井泉赵宝忠谢杰顾瑛刘凡光
Owner INST OF CHEM CHINESE ACAD OF SCI
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