Matrix type biochip CCD scanning fetch device

Abstract

This is a matrix pattern biological chip scanning reading device. It comprises stimulating-photon system, the releasing fluorescence collective subsystem, the X-Y scanning terrace sup system and the testing sub system. The stimulating photon system produce the single color even stimulated light shining field. The matrix pattern biological chip be carried on X-Y scanning terrace sub system, and be placed in the single color even stimulated light shining field, and be driven by the X-Y scanning terrace sub-system to finish whole scanning reading course. The releasing fluorescence collective sub system collect and separate after stimulated matrix pattern biological chip transmitted fluorescence picture, then exchange this picture into electrical signal and store, display it. This invention outstanding features is to use the infinitive non-copolymerizated focusing light pass way to simplify the structure. The CCD is photo-electrical exchanger can raise the exchange efficiency 50-60% and as between the stimulated light way and testing light way is 30 degree angle, reduce the noise of background. This invents suits with the sample's diameter 75-500mu meter matrix pattern biological chip.

Description

technical field [0001] The invention relates to a CCD scanning reading device for detecting the hybridization reaction result of a matrix biochip (Microarray). Background technique [0002] Matrix biochip (Microarray) can be widely used in drug screening and new drug development, disease diagnosis, environmental protection and so on. After hybridization reaction between the samples to be tested labeled with fluorescent dyes (commonly used fluorescent dyes: Cy3, Cy5, etc.) The emitted fluorescence is finally detected by the photoelectric conversion device to detect the intensity of the emitted fluorescence, and then interpret the corresponding biological information. [0003] At present, matrix biochip scanning and reading instruments usually use laser as the excitation light source to generate a monochromatic beam to excite and irradiate matrix biochips. The confocal optical system collects the fluorescence emitted by the chip, and PMT is collected by the photoelectric conv...

AI Technical Summary

Problems solved by technology

[0004] The structure of the confocal optical path adopted by the above-mentioned scanning and reading instrument is complicated, and the suppression of non-focal plane stray light by the confocal optical path does not improve the signal-to-noise ratio, but instead causes the system to be very sensitive to the surface unevenness of the matrix biochip; the laser light source Large size, high cost of replacing a new light source; the angle between the excitation light path and the detection light path is 0°, resulting in high background noise; PMT (photomultiplier tube) photoelectric conversion efficiency is low (<50%); The photodrifting phenomenon of the chip affects the repeated detection of the matrix biochip

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