Detection gene of Fasarium graminearum for resisting carbendazim and its detection method

A technology of Fusarium graminearum and carbendazim, which is applied in the fields of genetic engineering, plant genetic improvement, chemical instruments and methods, etc., can solve the problem of inability to use short-term prediction of drug resistance, limited number of test samples, difficult drug-resistant subgroups, etc. problem, to achieve the effect of effective detection, simple detection and monitoring, and simple method
CN1657627AInactive Publication Date: 2005-08-24NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2005-08-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

A Fusarium graminearum beta 2-microtubulin gene for detecting the resistance of Fusarium graminearum to carbendazim and its ASo-RCR detection method are disclosed. The whole length of said gene contains 997 nucleotides, one intron and the drug-resistant mutation site. It has high detecting speed (4 hr) and correctness (99%).
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Description

1. Technical field

[0001] The present invention is a detection gene and detection method of Fusarium graminearum (Gibberella zeae) resistant to carbendazim, which belongs to the detection method of plant pathogenic bacteria drug-resistant subgroups, and is specially used for detection of resistance to carbendazim and other benzene and imidazole fungicides of Fusarium graminearum. 2. Technical Background

[0002] Wheat scab is a worldwide disease caused by Fusarium graminearum, which seriously affects the yield and quality of wheat. For a long time, benzimidazole fungicides or compound agents based on such agents have been used for chemical control. Benzimidazole fungicides are used in production as a class of high-efficiency, broad-spectrum systemic fungicides, which solve the environmental toxicity problem of protective fungicides and improve the ability of humans to control the disease. Benzimidazole fungicides include carbendazim, benomyl, thiabendazole, and thiophanate...

Claims

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