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Heavy metal adsorbent composition

A heavy metal and adsorbent technology, applied in the direction of adding compounds to stimulate growth, microorganisms, microorganisms, etc., can solve the problems of limited use and high cost

Inactive Publication Date: 2005-10-12
KOWA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method is cheap, but produces a large amount of sludge containing high concentrations of heavy metals, so the sludge must be disposed of at the final disposal site
Although ion exchange produces only small amounts of sludge, its high cost limits its use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 (selection and identification of heavy metal adsorbing bacteria)

[0050] (1) Selection of heavy metal adsorbing bacteria

[0051] The soil was suspended in normal saline and allowed to stand, and the supernatant was cultured in BrainHeart Infusion Agar medium containing 1mM heavy metals, and the colonies that appeared were selected after 1 day.

[0052] (2) Identification of the obtained strain

[0053] a.method

[0054] The first phase of the bacteria test was to observe the morphology of the cells, Gram staining, presence or absence of spores, and presence or absence of flagellar movement with an optical microscope U-LH1000 (Olympus, Japan). Colony morphology was observed on a medium containing Brain Heart InfusionAgar (Becton Dickinson, NJ, USA) and agar (B.H.I agar). Catalase reaction, oxidase reaction, acid / gas generation from glucose, glucose oxidation / fermentation (O / F) tests were performed.

[0055] The second stage of the test for bacteria is ...

Embodiment 2

[0067] After culturing KRI-02, KRI-03, and KRI-04 in Brain Heart Infusin Medium (Difco), they were washed with water, and 0.5N hydrochloric acid was added in a volume equivalent to 5 times the wet weight of the bacteria to suspend them. Then, the bacteria added with hydrochloric acid were shaken at 37° C. for 2 hours. In addition, the method of Brierley et al. (USP 4,992,179) was studied comparatively. That is, 3% sodium hydroxide in a volume equivalent to 5 times the wet weight will be added, and the bacteria will be shaken at 50°C or 100°C for 10 minutes. After shaking, each strain was thoroughly washed with water and freeze-dried. The results are shown in Table 5. Compared with the case of washing with water (untreated), the acid treatment reduced the weight by 20%, while the sodium hydroxide treatment reduced the weight by more than 50%, especially when treated at 100°C, Weight reduction over 60%.

[0068] Approach

Embodiment 3

[0069] Embodiment 3 (measurement of metal adsorption amount)

[0070] The freeze-dried bacterial powder was dispersed in a buffer solution (Tris: 100 mM) to prepare a 60 mg / ml suspension. Add 20 microliters of bacterial suspension to 2.4 mM heavy metal aqueous solution (CdCl) prepared by Tris (10 mM). 2 , CuSO 4 , ZnCl 2 , NiCl 2 ) in 1 ml. Centrifuge after the reaction, and measure the heavy metal concentration in the separated supernatant with an atomic absorption photometer.

[0071] The results are shown in Tables 6-9. Compared with washing with water, the adsorption capacity of KRI-02, KRI-03, and KRI-04 after acid treatment increased to cadmium and copper. Although the cadmium adsorption of KRI-02, KRI-03, and KRI-04 treated with sodium hydroxide also increased compared with water washing, the acid treatment increased more than that of sodium hydroxide. Acid-treated KRI-02, KRI-03, KRI-04 showed increased adsorption of Zn and Ni than water-washed, but NaOH treatme...

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Abstract

A heavy metal adsorbent composition containing bacterial cells which are obtained by acid-treating a bacterium selected from among Bacillus sp. KRI-02 and its analogs, Bacillus licheniformis and Staphylococcus sp. KRI-04 and its analogs; and a method of removing a heavy metal from a heavy metal-containing medium with the use of the composition. Thus, heavy metals can be removed from rivers, lakes, ponds, industrial waste waters, etc.

Description

technical field [0001] The invention relates to a heavy metal adsorbent composition containing dead bacterial cells, and a method for removing heavy metals from heavy metal-containing media (such as environmental water) by using the heavy metal adsorbent composition. Background technique [0002] Environmental pollution caused by heavy metals such as cadmium, copper, zinc, chromium, and lead has become a worldwide problem because these metals are toxic to living things in trace concentrations. Coagulation precipitation is widely used as a means of removing heavy metals. Although this method is cheap, it produces a large amount of sludge containing high concentrations of heavy metals, so the sludge must be disposed of at the final disposal site. Although ion exchange produces only small amounts of sludge, its high cost limits its use. [0003] There have been many reports recently disclosing methods for removing heavy metals using biological substances such as bacteria, yea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22C12N1/38
CPCB01J20/22C12R1/07B01J2220/4868C12R1/10C12N1/38C12R1/44C12N1/205C12R2001/07C12R2001/10C12R2001/44B01J20/00C12N1/20
Inventor 铃木宏典中尾裕史
Owner KOWA CO LTD
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