Method for preparing ultra macropore type rigid macromolecule medium by using suspension liquid of fine particles of calcium carbonate

A calcium carbonate, suspension technology, applied in chemical instruments and methods, material separation, analysis of materials, etc., can solve problems such as reduced adsorption capacity

Inactive Publication Date: 2005-11-16
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Nucleic acid molecules cannot enter the interior of ordinary chromatographic...

Method used

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  • Method for preparing ultra macropore type rigid macromolecule medium by using suspension liquid of fine particles of calcium carbonate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Weigh 10 grams of nano-scale calcium carbonate powder, add it to 50 mL of 1% Triton 114 solution, shake it for 10 minutes, then mix it in an ultrasonic water bath for 30 minutes, then centrifuge it at 8000rpm for 10 minutes, pour off the supernatant before adding Distilled water, and then centrifuged, so repeated several times until Triton 114 is removed, and then set the volume to 50mL, you can get 20% calcium carbonate suspension.

[0023] Weigh 0.08 g of benzoin ether and 1.2 g of Span 80 and dissolve them in 4.5 mL of glycidyl methacrylate (GMA) and 3.0 mL of ethylene glycol dimethacrylate (EDMA); then add 2.5 mL of cyclohexanol and 1 mL of lauryl alcohol and mix well. Add 8mL of 20% calcium carbonate suspension to the mixed solution, stir for 5 minutes at 8000rpm, the small droplets formed by its emulsification can be clearly seen under the microscope, then add the stirred emulsion to 120mL of 2% In the PVA aqueous solution, stir at 1800 rpm for 1 minute, then tra...

Embodiment 2

[0025] Weigh 3.8 grams of calcium hydroxide solid powder, dissolve in sufficient water to obtain a clear calcium hydroxide solution, add the obtained calcium hydroxide solution into a three-neck reactor with a stirring paddle, and stir at a high speed While feeding carbon dioxide gas, stop feeding when the turbidity of the reaction no longer increases, then centrifuge to get the supernatant and use a clear calcium hydroxide solution to check whether the supernatant still contains calcium bicarbonate, if not, you can remove the supernatant. Clear liquid, resuspend the precipitate and adjust the volume to 50mL to obtain a 10% calcium carbonate suspension.

[0026] Weigh 0.08 g of benzoin ether and 1.5 g of Span 80 and dissolve them in 4.5 mL of glycidyl methacrylate (GMA), 3.0 mL of ethylene glycol dimethacrylate (EDMA); then add 3 mL of toluene and 2 mL of n-heptane to mix uniform. Add 4.5mL of 10% calcium carbonate suspension to the mixture, stir at 8000rpm for 5 minutes, the...

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Abstract

A process for preparing the rigid high-molecular medium with ultra-large pores from calcium carbonate microparticles suspension includes preparing suspension of calcium carbonate, mixing glicide methylacrylate, benzoin ether, span-80 and ethanediol dimethylacrylate, dispersing said suspension in the mixed solution to become emulsion, emulsifying in the aqueous solution of polyvinyl alcohol, rotating while polymerizing reaction under ultraviolet irradiation, and washing with water and absolute alcohol. It can be used for chromatography.

Description

technical field [0001] The invention relates to a preparation method of a super-macroporous polymer microsphere separation medium, which belongs to the preparation technology of biological macromolecule separation and purification medium. Background technique [0002] Liquid chromatography has become an important separation and purification tool for biomacromolecules because of its mild separation conditions and high separation efficiency. In the liquid chromatography of biomacromolecules, the performance of the separation medium often plays a decisive role, so obtaining a separation medium with excellent performance has always been one of the important research topics. [0003] In order to achieve the separation effect of high flow rate, high capacity and high resolution, people have been trying to find chromatographic media with excellent performance. In the last century, researchers discovered that when the pore size of the membrane reaches 0....

Claims

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Application Information

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IPC IPC(8): B01J20/26
Inventor 孙彦孙国勇董晓燕白姝史清洪
Owner TIANJIN UNIV
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