Polysaccharide material for promoting cell tactophily and preparation thereof
A cell adhesion and polysaccharide technology, applied in the field of biological products, can solve the problems of strong hydrophilicity and difficult plasticity, and achieve the effects of good molding, promoting cell adhesion, good biocompatibility and degradability
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[0033] Step 1: Extraction of pure Bletilla striata polysaccharide and preparation of solution
[0034] Take the tubers of Bletilla striata, a commercially available traditional Chinese medicine orchid plant, thoroughly crush them, boil them in water for 3 hours, boil them for 8-10 times, filter the filtrate with three layers of gauze, add twice the volume of absolute ethanol, and settle overnight; discard the supernatant by centrifugation, and repeat After three times, the precipitate was filtered by suction, dissolved in double distilled water, protein impurities were precipitated by the Sevag method, and no protein was detected by the Coomassie brilliant blue method repeatedly; extracted with anhydrous ether, and the aqueous phase was freeze-dried to obtain the crude Bletilla striata polysaccharide; the crude product was subjected to DE-52 Ion-exchange column (Whatman product) chromatography, anthrone-sulfuric acid method detects single elution peak, and freeze-drying obtains...
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