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Polysaccharide material for promoting cell tactophily and preparation thereof

A cell adhesion and polysaccharide technology, applied in the field of biological products, can solve the problems of strong hydrophilicity and difficult plasticity, and achieve the effects of good molding, promoting cell adhesion, good biocompatibility and degradability

Inactive Publication Date: 2005-12-21
THE AFFILIATED DRUM TOWER HOSPITAL MEDICAL SCHOOL OF NANJING UNIV
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Problems solved by technology

[0004] However, although polysaccharides have excellent biocompatibility and comprehensive and diverse biological effects, they have not been widely used in tissue engineering and other fields due to their shortcomings of strong hydrophilicity and difficult plasticity. field of biomaterials

Method used

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  • Polysaccharide material for promoting cell tactophily and preparation thereof
  • Polysaccharide material for promoting cell tactophily and preparation thereof
  • Polysaccharide material for promoting cell tactophily and preparation thereof

Examples

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Embodiment Construction

[0033] Step 1: Extraction of pure Bletilla striata polysaccharide and preparation of solution

[0034] Take the tubers of Bletilla striata, a commercially available traditional Chinese medicine orchid plant, thoroughly crush them, boil them in water for 3 hours, boil them for 8-10 times, filter the filtrate with three layers of gauze, add twice the volume of absolute ethanol, and settle overnight; discard the supernatant by centrifugation, and repeat After three times, the precipitate was filtered by suction, dissolved in double distilled water, protein impurities were precipitated by the Sevag method, and no protein was detected by the Coomassie brilliant blue method repeatedly; extracted with anhydrous ether, and the aqueous phase was freeze-dried to obtain the crude Bletilla striata polysaccharide; the crude product was subjected to DE-52 Ion-exchange column (Whatman product) chromatography, anthrone-sulfuric acid method detects single elution peak, and freeze-drying obtains...

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Abstract

The invention relates to the distillation and producing method of a kind of amylase material used for accelerating cell adhesive growth. The invention gets natural medicament bletilla striata tuber to have amylase component elementary distillation, eliminate albumen through small molecule hydrophobic substances, then through ion exchange chromatogram and gelatin filtration chromatogram to get pure distilled object bletilla striata amylase material, which is solid and dusty, then have antiseptic treatment. As a kind of amylase, the bletilla striata amylase got from separation and distillation of the natural medicament bletilla striata in the invention can affect with the extracellular matrix (ECM) components, and accelerate cell adhesive and cell wall adnate growth; meantime, the membrane cross-linked by bletilla striata amylase has good molding, strong tenacity, good biology compatibility and degeneration, so it's good choice in tissue engineerig material. It has solves the disadvantage which present technology can't overcome, that amylase substance has strong hydrophilicity and hard plasticity. The preparation method is simple, easy to control and low-cost.

Description

technical field [0001] The invention relates to a biological product, in particular to a method for extracting and preparing a polysaccharide material used to promote cell adhesion and growth. Background technique [0002] Biomaterials for tissue engineering have been studied for nearly two decades. Usually, high-concentration seed cells cultured in vitro are planted on natural or synthetic extracellular matrix carriers, compounded with inducing factors, and then transplanted in vivo to achieve the purpose of forming new functional tissues [Noshi, T et al. , "Artif Organs", Vol. 25, No. 3, pp. 201-208 (2001)]. Commonly used tissue engineering materials can be divided into two categories: synthetic materials and natural materials according to their sources. Synthetic materials include hydroxyapatite (HA), bioactive glass ceramics (BGC), polylactic acid (PLA), polyglycolic acid (PGA), etc., which have been widely used at present, and they are often used in combination to pla...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 孙剑涛张峻峰王春明刁华佳
Owner THE AFFILIATED DRUM TOWER HOSPITAL MEDICAL SCHOOL OF NANJING UNIV
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