Assaying method of Chinese medicine laying particle active principle and content

A technology of active ingredients and determination methods, applied in the field of analytical chemistry, can solve the problems of identification and content determination of active ingredient peptides in traditional Chinese medicine Luying granules, poor accuracy, low sensitivity, etc., and achieve accurate identification and content of active ingredient peptides The effect of determination, elimination of interference, and simple method

Inactive Publication Date: 2006-09-20
FUDAN UNIV
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The known technology generally uses Coomassie Brilliant Blue method to determine the content of active ingredient polypeptide in traditional Chinese medicine Luying Granules. This method has many shortcomings, such as: poor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Assaying method of Chinese medicine laying particle active principle and content
  • Assaying method of Chinese medicine laying particle active principle and content
  • Assaying method of Chinese medicine laying particle active principle and content

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0016] Example 1 Identification of the active ingredient polypeptide of traditional Chinese medicine Luying granules

[0017] Chromatographic conditions:

[0018] Agilent 1100 High Performance Liquid Chromatography / Mass Spectrometer (including G1322A Vacuum Degasser online degassing, G1312A Bin Pump high pressure double pump, G1313A ALS Autosampler automatic sampler, G1316AThermostatted Column Compartment column oven, G1315B Diode Array and Multiple Wavelength Detector, DAD diode Array detector, G1946D(SL)Qudarpole LC / MSD quadrupole mass spectrometer, G2710AA single instrument ChemStation for liquidchromatography(LC)systesm chromatography workstation), Agilent Zorbak, Eclipse C18 (150×2.1mm ID, 5μm); mobile phase: A: 0.1% trifluoroacetic acid water, B: 0.1% trifluoroacetic acid acetonitrile, 0 to 120 minutes A: B changed from 95:5 to 70:30; flow rate: 0.5ml·min -1 ; Detection wavelength: 220nm; column temperature: 25°C; injection 5μl.

[0019] Mass spectrometry conditions:

[0020...

Example Embodiment

[0025] Example 2 Determination of Polypeptide Content of Active Ingredient in Traditional Chinese Medicine Luying Granules

[0026] Chromatographic conditions:

[0027] American Angilent HP-1100 high performance liquid chromatograph, including online degassing, quaternary pump, column thermostat, UV detector, HP chromatography workstation and 20μL manual sampling system; Column: Agilent Zorbak, Eclipse C 18 (150×4.6mm ID, 5μm); column temperature: 25°C; mobile phase: A: 0.1% trifluoroacetic acid aqueous solution, B: 0.1% trifluoroacetic acid acetonitrile, 0 to 60 minutes A:B changes from 95:5 to 70:30; Flow rate: 1.0mL / min; Detection wavelength: 220nm. See the typical chromatogram figure 1 .

[0028] Preparation of the reference solution: accurately weigh the appropriate amount of the reference substance oxytocin (nonapeptide), add water to make a solution containing 0.25mg per 1ml, that is,

[0029] Preparation of the test solution: Take the appropriate amount of the contents of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to view more

Abstract

A method for discriminating the active component of Chinese elder flower particle and measuring its content features that the HPLC/MS method is used to discriminating the polypeptide as its active component and the efficient liquid-phase chromatography is used to measure the content of polypeptide.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a method for determining the effective components and content of traditional Chinese medicine Luying granules. Background technique [0002] Chinese patent medicine Luying, its Luying granule dosage form, has been on the market for many years. The main active ingredient is Xiaofen polypeptide, which is brown-yellow or tan granules, sweet and slightly bitter. Widely used in clinical internal medicine, it has the effects of soothing the liver and strengthening the spleen, promoting blood circulation and removing blood stasis, reducing swelling and relieving pain. [0003] The known technology generally uses the Coomassie Brilliant Blue method to determine the content of the active ingredient polypeptide in the traditional Chinese medicine Luying Granules. This method has many shortcomings, such as: poor accuracy, low sensitivity, and many interferences. So far, ther...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K36/35A61K9/16A61P29/00G01N30/02
Inventor 段更利黄滔敏刘振
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap