Low-temperature amylase strain, low-temperature amylase, and production therefor

A technology of low-temperature amylase and production method, which is applied in the field of producing the enzyme and amylase, and can solve problems such as difficulty in separation

Inactive Publication Date: 2007-04-25
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microbial strains that can produce low-temperature enzymes mainly exist in low-temperature natural ecological environments and are difficult to isolate

Method used

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  • Low-temperature amylase strain, low-temperature amylase, and production therefor
  • Low-temperature amylase strain, low-temperature amylase, and production therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1: the cultivation of low-temperature amylase-producing bacteria (LA140)

[0074] The low-temperature amylase-producing bacteria LA140 of the present invention is inoculated in broth medium containing soluble starch, wherein soluble starch 2%, beef extract 1%, peptone 1%, NaCl 0.5%, agar 3g, water 100mL, pH7, 0.1 MPa steam sterilization for 30 minutes. Cultivate at about 20°C for 2-3 days, add 0.4 mol / L I+2KI solution to develop color. Then an obvious transparent circle is formed around the colony of the low-temperature amylase-producing bacteria of the present invention.

Embodiment 2

[0075] Embodiment 2: the fermentation culture-I of low-temperature amylase producing bacterium (LA140 bacterial strain)

[0076] Inoculate the slant strain of the low-temperature amylase-producing bacteria of the present invention into the fermentation medium. Among them, peptone: 10g, corn flour: 15g, yeast extract: 2g, beef extract: 10g, sodium chloride: 1g, calcium chloride: 2g, ferrous sulfate: 0.1g, magnesium sulfate: 0.5g, water: 1000mL, pH7 .0. 30mL per 250ml Erlenmeyer bottle, 0.1MPa steam sterilization for 30 minutes. The culture conditions are: 20° C., 200 rpm, 36 hours of fermentation, and the enzyme activity reaches 28 U / mL.

Embodiment 3

[0077] Embodiment 3: Fermentation culture-II of low-temperature amylase-producing bacteria (LA140 bacterial strain)

[0078] The low-temperature amylase-producing bacteria LA140 was inoculated in broth peptone medium, in which 1% beef extract, 1% peptone, 0.5% NaCl, 1% soluble starch, pH 7.0, 0.1MPa steam sterilization for 25 minutes. After culturing for 36 hours at 20° C. and 150 rpm, the culture was added to the fermentation medium at a ratio of 15%.

[0079] The components of the fermentation medium are: peptone: 10g, corn flour: 15g, yeast extract: 2g, beef extract: 10g, sodium chloride: 1g, calcium chloride: 2g, ferrous sulfate: 0.1g, magnesium sulfate: 0.5g, Water: 1000mL. 0.1MPa steam sterilization for 25 minutes. 500ml Erlenmeyer bottle liquid 100mL. Shake culture at 20° C. and 200 rpm for 60 hours. After culturing, the crude enzyme solution of low-temperature amylase is obtained by centrifugal separation. The low-temperature amylase activity of the obtained amyla...

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PUM

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Abstract

The invention disclosed A.media which can produce low temperature amylase as well as its producing method. The invention has established the systematic method of preserving, rejuvenating and selecting the A.media. By optimizing the medium compounding and the fermenting technologies, the invention has established method to produce amylase. The low temperature amylase has high enzyme activity under low temperature. The invention has significant meanings and it can be used in paper, food and pharmaceutical industry such as cerealose, beer, yellow wine, glucose, aginomoto and antibiotics industries.

Description

field of invention [0001] The invention relates to the field of microorganisms and microbial fermentation. Specifically, the present invention relates to an amylase produced by a bacterium with a lower optimum enzymolysis temperature, a microbial strain producing the enzyme and a method for producing the enzyme. Background technique [0002] In industrial production, starch is hydrolyzed into products such as glucose by the catalysis of inorganic acid or enzyme. The use of enzymes is currently preferred, which can increase yields and bring various advantages such as beneficial economic benefits and social benefits. Enzymatic hydrolysis allows greater control over starch hydrolysis, reaction specificity and product stability. Mild reaction conditions include lower temperature, near-neutral pH, etc., thereby reducing energy consumption in production, reducing production cost and environmental pollution. [0003] Amylase is a biocatalyst with a wide range of uses, widely use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/28
Inventor 茆军张志东冯怀蓉王小红唐琦勇孙建娄恺魏东石玉瑚闫论
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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