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Process of extracting luteolin-O-beta-D-glucoside from elsholtzia bodinieri

A technology of luteolin and glucoside, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of reducing atherosclerosis and cholesterol, achieve high extraction rate, simple extraction process, and easy preparation convenient effect

Active Publication Date: 2010-05-12
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the systematic separation of the chemical components of Perilla, it was found that the content of luteolin-7-O-β-D-glucoside (luteolin-7-O-β-D-glucoside) was higher in Perilla , Luteolin-7-O-β-D-glucoside has antitussive, expectorant, anti-inflammatory effects, and has the effect of reducing experimental atherosclerosis and cholesterol in rabbits. Subcutaneous injection in rats can enhance capillary permeability However, there is no literature report on the extraction of luteolin-7-O-β-D-glucoside from Dongbasil

Method used

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  • Process of extracting luteolin-O-beta-D-glucoside from elsholtzia bodinieri
  • Process of extracting luteolin-O-beta-D-glucoside from elsholtzia bodinieri
  • Process of extracting luteolin-O-beta-D-glucoside from elsholtzia bodinieri

Examples

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Embodiment 1

[0014] Example 1, Eastern perilla is collected from Honghe Prefecture, Yunnan Province. 500 g of the whole plant of Eastern perilla is crushed, and 4000 ml of ethanol-water is used as the extractant, and the extraction is refluxed, wherein the volume percentage of ethanol is 70%, and the extract is concentrated under reduced pressure After ethanol is gone, the concentrated solution of Dongbasil extract is obtained, which is extracted three times with ethyl acetate equal to the volume of the concentrated solution, and the aqueous phase extracted with ethyl acetate is concentrated under reduced pressure to remove residual ethyl acetate, and then freeze-dried to obtain Osmanthus osmanthus The crude product of luteolin-7-O-β-D-glucoside, the content determined by HPLC and UV is 80%, and the crude product is crystallized and recrystallized to obtain pure luteolin-7-O-β-D-glucoside Product, HPLC, UV assay content is 96%.

Embodiment 2

[0015] Example 2: Grind 500 g of the whole herb of Eastern Perilla, use 7000 ml of ethanol-water as the extractant, and extract under reflux, wherein the volume percentage of ethanol is 90%, and the extract is concentrated under reduced pressure until there is no ethanol to obtain concentrated Eastern Perilla Extraction, the Eastern perilla extract is made into an aqueous suspension, extracted three times with chloroform equal to the volume of the suspension, the aqueous phase after the chloroform extraction is then extracted with 1-pentanol, and the 1-pentanol extraction phase is concentrated under reduced pressure, Vacuum drying, then get crude luteolin-7-O-β-D-glucoside, HPLC, UV assay content is about 61%, crystallize and recrystallize the crude product, then get luteolin-7-O-β - The pure product of D-glucoside, the content determined by HPLC and UV is 98%.

Embodiment 3

[0016] Example 3: Grind 500 g of the whole herb of Perilla basil, use 10,000 ml of acetone-water as the extractant, and extract under reflux, wherein the volume percentage of acetone is 60%, and the extract is concentrated under reduced pressure until there is no acetone, and then the extract of basil is obtained. Extract the concentrated solution with petroleum ether equal to the volume of the concentrated solution twice for degreasing, pass the water phase through the macroporous adsorption resin, wash with water until the effluent is colorless, use 95% ethanol as the eluent for elution, and concentrate the eluent under reduced pressure Afterwards, freeze-dry to obtain luteolin-7-O-β-D-glucoside fine product, and the HPLC and UV determination content is about 90%; carry out crystallization and recrystallization to crude product and fine product, then obtain luteolin-7 -O-β-D-glucoside pure product, HPLC, UV assay content is 99%. Among them, the macroporous adsorption resin i...

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Abstract

The present invention relates to extraction of medicine from plant material, and is especially process of extracting luteolin-7-O-beta-D-glucoside from Elsholtzia bodinieri. The process includes the following steps: extracting Elsholtzia bodinieri with solvent, dissolving the extracted concentrated solution or extractum in water, extracting and defatting with weak polar or medium polar organic extractant, extracting the defatted water phase with water immiscible low carbon alcohol extractant, concentrating and drying the extracted phase, and re-crystallizing to obtain luteolin-7-O-beta-D-glucoside. Or, the defatted water phase is adsorbed with macroporous resin and eluted with eluent, and the eluted liquid is concentrated, dried and re-crystallized to obtain luteolin-7-O-beta-D-glucoside.The extracting process is simple, low in power consumption, environment friendly and high in extracting rate, and the product is used in developing medicine for various diseases.

Description

technical field [0001] The invention relates to a method for extracting medicines from plants, in particular to a method for extracting luteolin-7-O-β-D-glucoside from Dong Perilla. Background technique [0002] Eastern perilla (Elsholtzia bodinieri Vaniot.) is a plant of the genus Elsholtzia in the family Labiatae. The whole herb can be used as medicine to treat cold and cold, headache and body pain, toothache due to deficiency fire, diarrhea, acute conjunctivitis, hepatitis, sore throat, indigestion, urinary retention and other symptoms; the young leaves can be used as tea, which has the effect of clearing away heat and detoxification. The effect. In the systematic separation of the chemical components of Perilla, it was found that the content of luteolin-7-O-β-D-glucoside (luteolin-7-O-β-D-glucoside) was higher in Perilla , Luteolin-7-O-β-D-glucoside has antitussive, expectorant, anti-inflammatory effects, and has the effect of reducing experimental atherosclerosis and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08A61K36/53A61K127/00
Inventor 赵东保刘绣华李明静张卫邵鹏
Owner HENAN UNIVERSITY
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