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Preparing process of carrageenan oligose sulfate with antioxidant activity

A technology of antioxidant activity and carrageenan oligosaccharides, which is applied in the direction of anti-toxic agents, fermentation, drug combination, etc., can solve the problems of low product yield, uneasy control of reaction conditions, and easy damage to product structure, and achieve high product yield, The effect of stable quality and simple preparation process

Inactive Publication Date: 2007-06-27
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, there have been reports on the activity of carrageenan sulfate, including inhibition of intestinal flora, improvement of immune function, anti-oxidation and protection against radiation damage, etc., but these reports all use chemical methods to degrade carrageenan to prepare carrageenan Sulphate, due to the uneasy control of the reaction conditions, the low yield of the product, and the easy damage of the product structure during the preparation process, the chemical method severely restricts the development and application of the activity of carrageenan oligosaccharide sulfate.
There are only reports on the preparation of active carara oligosaccharide sulfate by enzymatic hydrolysis: a preparation method of sulfated galactooligosaccharides with antitumor activity (ZL03138975.9), but there is no report on the preparation of galactooligosaccharides with antioxidant activity by enzymatic hydrolysis. cararaoligosaccharide sulfate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Dissolve 2g of κ-carrageenan in 100ml of sterile distilled water, add 12 units of κ-carrageenan enzyme powder made by cellulophage, 1 unit of enzyme activity is defined as producing 1 μmol of reducing sugar per minute at 32°C (calculated as galactose) the amount of enzyme required. Shake and react at 32°C at 150r / min for 18h, then concentrate the reaction solution to 20ml by rotary evaporation at 60°C, then add 4 times the volume of acetone to precipitate the oligosaccharide component, collect the precipitate by centrifugation, and dry it at 70°C to obtain kala oligosaccharides. Under ice-salt bath conditions, add 50ml of N,N-dimethylformamide (DMF) to a dry three-necked flask and keep stirring, and slowly add 15ml of chlorosulfonic acid dropwise with a constant pressure separatory funnel to react. Formation of DMF-chlorosulfonic acid sulfating reagent. Add 2g of dried carara oligosaccharides to a dry three-neck flask, add 80ml of DMF and stir to fully disperse the ol...

Embodiment 2

[0010] Dissolve 2g of κ-carrageenan in 100ml of sterile distilled water, add 9 units of κ-carrageenase powder made by cellulophage, shake and react at 150r / min at 34°C for 24h, and concentrate the reaction solution by rotary evaporation at 70°C to about 20ml, then add 10 times the volume of ethanol to precipitate the oligosaccharide component, collect the precipitate by centrifugation, and dry it at 76°C to obtain carara oligosaccharide. Add 2g of dried carara oligosaccharides to a dry three-necked bottle, add 50ml of DMF and stir to fully disperse the oligosaccharides in the solvent, then add 12ml of DMF-chlorosulfonic acid sulfating reagent, and gradually heat up to 70°C under water bath conditions , reaction 1h. After the reaction solution is cooled, add 10ml of deionized water, pour it into a beaker, add ethanol and stir to obtain a precipitate, vacuum filter, wash repeatedly with ethanol, and dry at 50°C to obtain yellow cararaoligosaccharide sulfate.

[0011] The above ...

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PUM

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Abstract

The process of preparing carrageenan oligose sulfate with antioxidant activity includes the steps of compounding kappa-carrageenin substrate, adding kappa-carrageenin enzyme powder to produce enzymolysis reaction, rotary evaporation and concentration, and organic solvent precipitation to collect produced carrageenan oligose. The process features the sulfating modification of carrageenan oligose, repeated washing with acetone and vacuum filtering and drying. The present invention with combined enzymolysis and chemical modification has simple preparation process, high product yield, stable quality and no destruction of the active group, and the product has antioxidant activity and may be used in antioxidant food.

Description

technical field [0001] The invention relates to an organic polymer, in particular to a preparation method of cararaoligosaccharide sulfate with antioxidant activity. Background technique [0002] At present, there have been reports on the activity of carrageenan sulfate, including inhibition of intestinal flora, improvement of immune function, anti-oxidation and protection against radiation damage, etc., but these reports all use chemical methods to degrade carrageenan to prepare carrageenan Sulphate esters, chemical methods are difficult to control the reaction conditions, the product yield is low, and the product structure is easily damaged during the preparation process, which seriously restricts the active development and application of cararaoligosaccharide sulfate esters. There are only reports on the preparation of active carara oligosaccharide sulfate by enzymatic hydrolysis: a preparation method of sulfated galactooligosaccharides with anti-tumor activity (ZL0313897...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12P19/00A61P39/06
Inventor 牟海津江晓路刘志鸿
Owner OCEAN UNIV OF CHINA
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