Protein identification from protein product ion spectra
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[0078] The present invention is illustrated by the following example. It is to be understood that the particular examples, materials, amounts, and procedures are to be interpreted broadly in accordance with the scope and spirit of the invention as set forth herein.
Gas-Phase Concentration, Purification and Identification of Whole Proteins from Complex Mixtures
[0079] In this example, we demonstrate that the ion parking approach can be used to facilitate the gas-phase concentration and purification of selected protein ions from a complex protein mixture for subsequent dissociation in a quadrupole ion trap mass spectrometer. Five proteins present in a relatively complex mixture derived from a whole cell lysate fraction of E. coli containing about 30 components were concentrated, purified and dissociated in the gas-phase, using a quadrupole ion trap mass spectrometer. Concentration of intact protein ions was effected using gas-phase ion / ion proton transfer reactions in conjunction with...
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