Amphiphilic entity nanoparticles
a technology of amphiphilic entities and nanoparticles, which is applied in the direction of antibody medical ingredients, peptide/protein ingredients, drug compositions, etc., can solve the problems of high toxic and expensive each of them, low yield of nanoparticles, and the standard method of generating polymer nanoparticles is slow, so as to reduce the overall cost of the synthetic reaction, replace or minimize the requirement, the effect of increasing the speed and reaction yield
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example 1
Formulation of a Self-Assembling Pullulan and Polycaprolactone Nanosphere
[0236]A mixture of 2.5 g of soybean oil and 2.5 g polysorbate 80 (Tween-80) was prepared. The mixture was stirred and heated at 40° C. for 5 minutes. 50 ml deionized water was added, and the resulting mixture was stirred and heated at 40° C. for 10 minutes. 5 ml DMSO containing 0.905 g pullulan and polycaprolactone was added, and the resulting mixture was stirred and heated at 45° C. for 10 minutes. 5 ml was taken for a pre-process sample. The remaining mixture was microfluidized in a single pass at 24,000 psi. The particle size of the pre-process sample was >4000 nm. The particle size after microfluidization was 155 nm.
example 2
Sample Preparation for Microfluidized Sample (Per Sample)
[0237]A mixture of 100 μl of microfluidized sample and 900 μl of reagent (0.1 M sodium phosphate buffer, 1 mM EDTA, 0.25% Triton X-100, 160 IU / mL of triglyceride hydrolase, and 1 IU / ml of cholesterol esterase) was prepared in a 8 ml glass vial.
[0238]The resulting mixture was incubated at ambient temperature in the dark for 1 hour. 100 μl of 5% sodium dodecyl sulfate was added, and the resulting mixture was vortexed for 30 seconds. 1 ml of ethanol was added, and the resulting mixture was vortexed for 30 seconds. 100 μl of an internal standard was added, and the resulting mixture was vortexed for 30 seconds. 4 ml of a 1:1 mixture of hexane:ether with 1% ethanol and 0.1% BHT was added. Ethanol and BHT stabilize the ether to prevent peroxide formation. The resulting mixture was vortexed for 60 seconds then centrifuged for 2 minutes on medium speed. The supernatant was extracted with a glass pipette and was stored at −80° C. for up...
example 3
Botulinum Toxin A Formulation with Pullulan and Polycaprolactone
[0239]A mixture of 1.6 g of soybean oil and 1.6 g of polysorbate 80 (Tween-80) is prepared and stirred for five minutes. In a separate container, a mixture of 100 ng of botulinum toxin A and 20 ml 0.9% saline is prepared and stirred for five minutes. The mixture of saline and botulinum toxin A is added to the mixture of oil and Tween-80 and stirred for 10 minutes. 5 ml of DMSO containing 0.905 grams pullulan and polycaprolactone are added, and the resulting mixture is stirred for 10 minutes. A 5 ml pre-process sample is taken. The remaining mixture is microfluidized in a single pass at 24,000 psi. The particle size before and after microfluidization is measured.
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