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Composition having lactobacillus plantarum strain gmnl-662 for promoting bone regrowth

Inactive Publication Date: 2018-08-23
GENMONT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides a composition containing Lactobacillus Plantarum strain GMNL-662. This strain can be administered through various paths to enter the digestive system and promote bone growth by increasing the gene expression of osteogenesis-related cytokine TGF-β and osteocalcin, while inhibiting the expression of osteoclast-related genes (such as TRAP-5). The strain also has the ability to improve the expression of osteogenic genes and slow down bone loss.

Problems solved by technology

Osteoporosis is a kind of systemic skeletal disease, which includes bone loss and bone tissue microstructure deterioration, resulting in bone fragility and risk of fracture.
Once the bone resorption is over bone formation, bone loss will caused, and finally result in osteoporosis.
The drugs that control osteoporosis are accompanied by some side effects.
It is found in the clinical trials that the use of drugs in combination has no addition effect, but will resist each other, or increase the incidence or strength of the side effects.
Therefore, the current guidelines for various prevention and treatment of osteoporosis are not recommended to use two anti-loss reagents, or use one anti-loss reagent together with one promoting osteoblast reagent.
The osteoporotic drugs clinically used in the elderly and menopausal women, such as Fosamax, Tevanate, Covaxin (bisphosphonates drugs), will cause serious necrosis of jaw bone joint if users do not pay attention to oral hygiene, or the users are subject to tooth extraction, dental implant surgery.
Recent studies have also found that it may cause the adverse reactions including atypical femoral fracture.

Method used

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  • Composition having lactobacillus plantarum strain gmnl-662 for promoting bone regrowth
  • Composition having lactobacillus plantarum strain gmnl-662 for promoting bone regrowth
  • Composition having lactobacillus plantarum strain gmnl-662 for promoting bone regrowth

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Experimental program
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Effect test

experiment 1

Analysis

[0026]Strain: Lactobacillus plantarum Strain GMNL-662

[0027]Strain Treatment:

[0028](1) preparation of viable bacteria: Inoculating the Lactobacillus Fermentum GMNL-296 from a frozen tube to 1 ml of MRS broth, and standing under 37° C. for aerobically incubating for 20 hours. The next day, adding 15 μl culture solution into 1.5 ml of MRS broth (1% secondary activation), and then standing under 37° C. for aerobically incubating for 20 hours. Estimating the bacteria number by using OD 600 to adjust the bacteria concentration to 8×107 CFU / ml.

[0029](2) preparation of dead bacteria: Inoculating the Lactobacillus Plantarum strain GMNL-662 from a frozen tube to 1 ml of MRS broth, and standing under 37° C. for aerobically incubating for 20 hours. The next day, adding 15 μl culture solution into 1.5 ml of MRS broth (1% secondary activation), and then standing under 37° C. for aerobically incubating for 20 hours. Estimating the bacteria number by using OD 600 to adjust the bacteria conc...

experiment 2

MNL-662 on Osteogenic Genes, Cytokines, and Osteoclast Genes

[0036]Extraction of tibial RNA: The left femur of the mice were removed, cut into small pieces with scissor, and an appropriate amount of liquid nitrogen was added to grind the bones quickly. The ground bone powder was added to 0.5 ml TRIzol® Reagent to extract RNA; 0.1 ml chloroform was then added thereto to turn up and down 15 times. The solution was placed at room temperature to react for 5 minutes, followed by centrifugalized and extracted the upper layer to new microcentrifuge tubes (eppendorf); 0.25 ml isopropanol was added thereto and the solution was placed at room temperature for 10 minutes and then centrifugalized; the supernatant was removed and the precipitate was washed with 0.5 ml 75% ethanol; after the precipitate was dried, 20-50 μl DEPC water was added to dissolve the precipitate and the RNA concentration was measured.

[0037]RNA reverse transcription cDNA: 1-5 μg RNA was obtained and RNase-free water was add...

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Abstract

A composition having Lactobacillus Plantarum strain GMNL-662 for promoting bone regrowth is provided. The Lactobacillus Plantarum strain GMNL-662 has an ability to promote the expression of osteogenic genes, inhibit the expression of osteoclast related genes, and promote the expression of osteogenesis-related cytokine TGF-β, so that the bone loss is improved.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application claims the benefit of the filing date of Taiwan patent application No. 106105625, filed on Feb. 20, 2017, the disclosure of which is incorporated herein by reference.FIELD OF THE INVENTION[0002]The present invention relates to a composition including Lactobacillus Plantarum strain GMNL-662 for promoting bone regrowth, and in particular relates to a composition including Lactobacillus Plantarum strain GMNL-662 which has an ability of increasing the expression of osteogenic genes.BACKGROUND OF THE INVENTION[0003]Osteoporosis is a kind of systemic skeletal disease, which includes bone loss and bone tissue microstructure deterioration, resulting in bone fragility and risk of fracture.[0004]During bone remodeling process, the bone formation of osteoblasts and bone resorption of osteoclast maintain the dynamic balance of bone tissue together. Once the bone resorption is over bone formation, bone loss will caused, and finally re...

Claims

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Application Information

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IPC IPC(8): A61K35/747A23L33/135
CPCA61K35/747A23Y2220/67A23V2002/00A23L33/135C12N1/20C12R2001/25C12N1/205A23V2400/169
Inventor CHEN, YI-HSINGTSAI, WAN-HUACHOU, CHIA-HSUANLIN, CHI CHIEN
Owner GENMONT BIOTECH
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