The invention provides a nursing disinfectant used in the obstetrics and gynecology department and a preparation method thereof and belongs to the technical field of nursing drugs in the obstetrics and gynecology department. The nursing disinfectant is prepared from, by weight, 6-8% of Chinese herbal medicine extract, 1-2% of lavender essential oil, 0.5-1.5% of glycerin, 0.1-0.5% of polyethylene glycol, 3-6% of povidone iodine and the balance sterile water, wherein the Chinese herbal medicine extract includes a hive, polygala, a glossy privet fruit, cortex phellodendri, scutellaria baicalensis, yams, butterflybush flowers, ash barks, a dahurian patrinia herb, radix ampelopsis, a barbed skullcap herb, Chinese bulbul, angelica and licorice. The prepared nursing disinfectant has the effects of sterilizing, detoxifying, clearing heat and dry dampness, diminishing inflammation, relieving itching, nourishing and invigorating blood circulation, is safe in use, non-irritant, free of anaphylactic reaction and good in nursing property.