The invention discloses a preparation method of a glass fiber reinforced plastic cable protection tube. The preparation method comprises preparation of equipment, preparation of a core mold, preparation of glue solution, preparation of fibers, winding forming, curing, demoulding, surface finishing and the like. According to the glass fiber reinforced plastic cable protection tube, alkali-free glass fiber is adopted as a reinforcing material, epoxy resin is used as a matrix, and the raw material cost is low; the glass fiber reinforced plastic cable protection tube is prepared by fiber winding,so that the mechanical automation degree is high, and the production efficiency is high; and compared with a metal steel tube, the prepared glass fiber reinforced plastic cable protection tube is light in weight, high in strength, good in toughness, excellent in corrosion resistance and electrical insulation performance, and long in service life, and can be safely applied to a power transmission line and telecommunication line dense area.