Deep-processing method of using starch watewater
A technology for starch wastewater and wastewater, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problems of wastewater pollution, resource utilization, etc., save raw material costs, not easy to imitate, The effect of saving waste water treatment costs and water costs
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Embodiment 1
[0025] (1) Strain preparation:
[0026] Preparation of high-yielding strains of polyunsaturated fatty acid oil: the refrigerator-preserved slant strain Cunninghamella echinulata (Fg) (Cunninghamella echinulata), Monoblepharis polymorpha Comu (Ma) and its mutant strain (Me) (Md) insert PDA (potato solid medium) slant respectively and carry out activation, cultivate 7d, get mycelium and insert and carry out liquid culture in the 250ml shaking flask that fills 100ml PDY (potato liquid) medium. The rotation speed is 120-150r / min, after culturing at 25-28°C for 2 days, take the inoculum size (volume fraction v / v) 5-10%, transfer once under the same liquid culture conditions to obtain liquid shake flask species, and then according to the reactor conditions Proceed to seed preparation.
[0027] (2) Liquid fermentation production
[0028] Add edible sugar at 20g / L to potato starch wastewater, press MgSO 4 2g / l, MnSO 4 2.5g / l, (NH 4 ) 2 SO 4 0.8g / l ratio was added to enhancer ...
Embodiment 2
[0030] Add edible sugar to the sweet potato starch wastewater by 20g / L, press MgSO 4 3g / l, MnSO 4 1g / l, (NH 4 ) 2 SO 41g / l ratio is added promotor, forms fermented medium, adds polyunsaturated oil high-yielding bacterium monochaete polymorpha (Ma) seed liquid prepared in embodiment 1 by 5% (v / v), at 25 ℃ of temperature, aeration Amount of 1:1.5, 7 days of fermentation under the condition of rotating speed 120r / min, mycelial biomass is 19.8g / L, oil content is 7.9g / L, myristic acid in oil is 0.50%, palmitic acid is 15.85%, palmitoleic acid 1.38%, stearic acid 3.30%, oleic acid 10.56%, linoleic acid 12.47%, γ-linolenic acid 9.80%, arachidic acid 0.30%, arachidonic acid 0.72%, arachidienoic acid 0.89%, arachidonic acid 35.23% %, EPA 0.80%, DHA 1.98%.
Embodiment 3
[0032] Add edible sugar to cornstarch waste water at 10g / L, press MgSO 4 2g / l, MnSO 4 2.5g / l, (NH 4 ) 2 SO 4 1g / l ratio adds promotor, forms fermented medium, adds the polyunsaturated oil high-yielding bacterium monochaete polymorpha mutagen (Me) seed liquid prepared in embodiment 1 by 3% (v / v), at temperature 25 ℃, ventilation rate 1:1.5, and rotation speed 80r / min under the conditions of fermentation for 7 days, mycelium biomass was 18.5g / L, oil content was 8.5g / L, myristic acid in oil was 0.27%, palmitic acid was 14.85%, Palmitoleic acid 1.38%, stearic acid 3.30%, oleic acid 20.98%, linoleic acid 12.47%, γ-linolenic acid 9.80%, arachidic acid 0.30%, arachidic acid 0.78%, arachidic acid 0.20, EPA 7.60% , DHA 2.08%.
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