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Deep-processing method of using starch watewater

A technology for starch wastewater and wastewater, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problems of wastewater pollution, resource utilization, etc., save raw material costs, not easy to imitate, The effect of saving waste water treatment costs and water costs

Inactive Publication Date: 2007-11-14
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Realize the ecologicalization of the starch processing industry, and solve the pollution and resource utilization of wastewater in the starch processing process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Strain preparation:

[0026] Preparation of high-yielding strains of polyunsaturated fatty acid oil: the refrigerator-preserved slant strain Cunninghamella echinulata (Fg) (Cunninghamella echinulata), Monoblepharis polymorpha Comu (Ma) and its mutant strain (Me) (Md) insert PDA (potato solid medium) slant respectively and carry out activation, cultivate 7d, get mycelium and insert and carry out liquid culture in the 250ml shaking flask that fills 100ml PDY (potato liquid) medium. The rotation speed is 120-150r / min, after culturing at 25-28°C for 2 days, take the inoculum size (volume fraction v / v) 5-10%, transfer once under the same liquid culture conditions to obtain liquid shake flask species, and then according to the reactor conditions Proceed to seed preparation.

[0027] (2) Liquid fermentation production

[0028] Add edible sugar at 20g / L to potato starch wastewater, press MgSO 4 2g / l, MnSO 4 2.5g / l, (NH 4 ) 2 SO 4 0.8g / l ratio was added to enhancer ...

Embodiment 2

[0030] Add edible sugar to the sweet potato starch wastewater by 20g / L, press MgSO 4 3g / l, MnSO 4 1g / l, (NH 4 ) 2 SO 41g / l ratio is added promotor, forms fermented medium, adds polyunsaturated oil high-yielding bacterium monochaete polymorpha (Ma) seed liquid prepared in embodiment 1 by 5% (v / v), at 25 ℃ of temperature, aeration Amount of 1:1.5, 7 days of fermentation under the condition of rotating speed 120r / min, mycelial biomass is 19.8g / L, oil content is 7.9g / L, myristic acid in oil is 0.50%, palmitic acid is 15.85%, palmitoleic acid 1.38%, stearic acid 3.30%, oleic acid 10.56%, linoleic acid 12.47%, γ-linolenic acid 9.80%, arachidic acid 0.30%, arachidonic acid 0.72%, arachidienoic acid 0.89%, arachidonic acid 35.23% %, EPA 0.80%, DHA 1.98%.

Embodiment 3

[0032] Add edible sugar to cornstarch waste water at 10g / L, press MgSO 4 2g / l, MnSO 4 2.5g / l, (NH 4 ) 2 SO 4 1g / l ratio adds promotor, forms fermented medium, adds the polyunsaturated oil high-yielding bacterium monochaete polymorpha mutagen (Me) seed liquid prepared in embodiment 1 by 3% (v / v), at temperature 25 ℃, ventilation rate 1:1.5, and rotation speed 80r / min under the conditions of fermentation for 7 days, mycelium biomass was 18.5g / L, oil content was 8.5g / L, myristic acid in oil was 0.27%, palmitic acid was 14.85%, Palmitoleic acid 1.38%, stearic acid 3.30%, oleic acid 20.98%, linoleic acid 12.47%, γ-linolenic acid 9.80%, arachidic acid 0.30%, arachidic acid 0.78%, arachidic acid 0.20, EPA 7.60% , DHA 2.08%.

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Abstract

The present invention discloses a method for producing microbial polyunsaturated fatty acid by utilizing lots of waste water produced in the starch production process. It is characterized by that said invention uses the described waste water as basic culture medium, adds partial nutrient and regulation metal ions, after sterilization the screening-obtained microbial polyunsaturated fatty acid high-yield strain can be inoculated, after the optimum fermentation process is adopted to make fermentation, then the hypholoma can be filtered, and the microbial fat with obvious function can be extracted, and the discharged water can be controlled under a certain COD load, and can be reused in the starch production process.

Description

technical field [0001] The invention relates to a method for deep processing by using starch wastewater, in particular, the invention provides a method for producing microbial polyunsaturated fatty acid oil by using starch wastewater. Background technique [0002] Starch is widely used in many industries and has formed a large scale. According to a recent international food and agricultural research report on world starch production by Rabobank, the total world starch production in 2003 was about 49 million tons. Among them, about 39.4 million tons of corn starch accounted for 80.4% of the total, about 4.1 million tons of wheat starch accounted for 8.36%, about 3 million tons of cassava starch accounted for 6.12%, and about 2.6 million tons of potato starch accounted for 5.3%. [0003] A large amount of water is needed in the starch production process. Generally, more than 10 tons of water are needed to produce 1 ton of starch. Most of this starch wastewater contains rich or...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/00C02F3/34
Inventor 张晓昱王宏勋
Owner HUAZHONG UNIV OF SCI & TECH
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