Biotic bone tissue engineering stent and its preparation method

Abstract

A tissue-engineered bionic bone scaffold with high biocompatibility, bioactivity, degradability and osteoplastic induction has a three-layer structure composed of external degradable compact biofilm layer, central porous layer with less pore diameter for high mechanical strength, and internal porous layer with big pore diameter for transferring nutrients. Said central and internal layers are a composition consisting of in-situ formed nano-phase Ca-P salt and degradable biologic material.

Description

technical field

[0001] The invention relates to a bionic bone tissue engineering scaffold and a preparation method thereof, belonging to the technical field of biomedical materials and tissue engineering. Background technique

[0002] The treatment of bone defect is one of the common clinical problems. The bone defect brings great inconvenience to the life of the patient and seriously affects the daily life. Commonly used clinical treatment methods such as autologous bone transplantation and allogeneic bone transplantation have limited donors, immune rejection and risk of disease transmission. The use of tissue engineering to prepare bone substitutes to promote bone regeneration has become a new method for the treatment of bone defects. The main method of tissue engineering is to adsorb and inoculate normal tissue cells cultured in vitro on a porous scaffold material with good biocompatibility and can be degraded and absorbed by the body, and then implant the cell material ...

Examples

Embodiment 1

[0029] a) Weigh 2g of chitosan, dissolve it in 50ml of 1.2% acetic acid aqueous solution, and stir until completely dissolved. Take 12ml of 0.1mol / l Ca(NO 3 ) 2 solution and 12ml of 0.06mol / l (NH 4 ) 2 HPO 4 The solution, under strong stirring, is added drop by drop to the chitosan solution in order to ensure that it can be uniformly dispersed as soon as possible after adding a small amount of solution each time, and to avoid the precipitation of chitosan caused by the increase of pH value caused by the local concentration being too high . Stir well to make a completely homogeneous solution. Stand overnight at 4°C or degas with a vacuum pump.

[0030] b) Add the solution in step a) into the cavity 4 of the mold with a syringe, and add slowly to avoid air bubbles. Cover the mold cover 1, put it vertically and slowly into liquid nitrogen, take it out after freezing for 12 hours, remove the top cover 1, bottom cover 2 and stainless steel outer tube 3 after thawing slightly...

Embodiment 2

[0039] a) Weigh 1g of chitosan and 1g of collagen, dissolve them in 50ml of 1.2% acetic acid aqueous solution, and stir until completely dissolved. Take 12ml of 0.1mol / l Ca(NO 3 ) 2 solution and 12ml of 0.1mol / l (NH 4 ) 2 HPO 4 The solution, under strong stirring, is added drop by drop to the chitosan collagen mixed solution in order to ensure that it can be uniformly dispersed as soon as possible after adding a small amount of solution each time, and to avoid the chitosan caused by the increase of the pH value caused by the local concentration being too high. precipitation. Stir well to make a completely homogeneous solution. Stand overnight at 4°C or degas with a vacuum pump.

[0040] b) Add the solution obtained in a) into the cavity 4 of the mold with a syringe, and add slowly to avoid air bubbles. Cover the mold cover 1, place it vertically at -20°C, take it out after freezing for 24 hours, remove the top cover 1, bottom cover 2 and stainless steel outer tube 3 aft...

Embodiment 3

[0048] a) Weigh 1 g of chitosan, dissolve it in 50 ml of 1.2% acetic acid aqueous solution, and stir until it is completely dissolved. An appropriate amount of 2% gelatin aqueous solution was added in the chitosan solution, stirred for 2 hours at 50°C and mixed evenly, and 12ml of 0.1mol / l Ca(NO 3 ) 2 solution and 12ml of 0.05mol / l (NH 4 ) 2 HPO 4 The solution, under strong stirring, is added drop by drop to the chitosan solution in order to ensure that it can be uniformly dispersed as soon as possible after adding a small amount of solution each time, and to avoid the precipitation of chitosan caused by the increase of pH value caused by the local concentration being too high . Stir well to make a completely homogeneous solution. Stand overnight at 4°C or degas with a vacuum pump.

[0049] b) Add the solution obtained in a) into the cavity 4 of the mold with a syringe, and add slowly to avoid air bubbles. Cover the mold cover 1, put it vertically and slowly into liquid...

technical field

[0001] The invention relates to a bionic bone tissue engineering scaffold and a preparation method thereof, belonging to the technical field of biomedical materials and tissue engineering. Background technique

[0002] The treatment of bone defect is one of the common clinical problems. The bone defect brings great inconvenience to the life of the patient and seriously affects the daily life. Commonly used clinical treatment methods such as autologous bone transplantation and allogeneic bone transplantation have limited donors, immune rejection and risk of disease transmission. The use of tissue engineering to prepare bone substitutes to promote bone regeneration has become a new method for the treatment of bone defects. The main method of tissue engineering is to adsorb and inoculate normal tissue cells cultured in vitro on a porous scaffold material with good biocompatibility and can be degraded and absorbed by the body, and then implant the cell material ...

Examples

Embodiment 1

[0029] a) Weigh 2g of chitosan, dissolve it in 50ml of 1.2% acetic acid aqueous solution, and stir until completely dissolved. Take 12ml of 0.1mol / l Ca(NO 3 ) 2 solution and 12ml of 0.06mol / l (NH 4 ) 2 HPO 4 The solution, under strong stirring, is added drop by drop to the chitosan solution in order to ensure that it can be uniformly dispersed as soon as possible after adding a small amount of solution each time, and to avoid the precipitation of chitosan caused by the increase of pH value caused by the local concentration being too high . Stir well to make a completely homogeneous solution. Stand overnight at 4°C or degas with a vacuum pump.

[0030] b) Add the solution in step a) into the cavity 4 of the mold with a syringe, and add slowly to avoid air bubbles. Cover the mold cover 1, put it vertically and slowly into liquid nitrogen, take it out after freezing for 12 hours, remove the top cover 1, bottom cover 2 and stainless steel outer tube 3 after thawing slightly...

Embodiment 2

[0039] a) Weigh 1g of chitosan and 1g of collagen, dissolve them in 50ml of 1.2% acetic acid aqueous solution, and stir until completely dissolved. Take 12ml of 0.1mol / l Ca(NO 3 ) 2 solution and 12ml of 0.1mol / l (NH 4 ) 2 HPO 4 The solution, under strong stirring, is added drop by drop to the chitosan collagen mixed solution in order to ensure that it can be uniformly dispersed as soon as possible after adding a small amount of solution each time, and to avoid the chitosan caused by the increase of the pH value caused by the local concentration being too high. precipitation. Stir well to make a completely homogeneous solution. Stand overnight at 4°C or degas with a vacuum pump.

[0040] b) Add the solution obtained in a) into the cavity 4 of the mold with a syringe, and add slowly to avoid air bubbles. Cover the mold cover 1, place it vertically at -20°C, take it out after freezing for 24 hours, remove the top cover 1, bottom cover 2 and stainless steel outer tube 3 aft...

Embodiment 3

[0048] a) Weigh 1 g of chitosan, dissolve it in 50 ml of 1.2% acetic acid aqueous solution, and stir until it is completely dissolved. An appropriate amount of 2% gelatin aqueous solution was added in the chitosan solution, stirred for 2 hours at 50°C and mixed evenly, and 12ml of 0.1mol / l Ca(NO 3 ) 2 solution and 12ml of 0.05mol / l (NH 4 ) 2 HPO 4 The solution, under strong stirring, is added drop by drop to the chitosan solution in order to ensure that it can be uniformly dispersed as soon as possible after adding a small amount of solution each time, and to avoid the precipitation of chitosan caused by the increase of pH value caused by the local concentration being too high . Stir well to make a completely homogeneous solution. Stand overnight at 4°C or degas with a vacuum pump.

[0049] b) Add the solution obtained in a) into the cavity 4 of the mold with a syringe, and add slowly to avoid air bubbles. Cover the mold cover 1, put it vertically and slowly into liquid...

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