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Method for producing L-lactic acid and coagulate bacillus cereus special for the same

A technology of Bacillus coagulans and lactic acid, applied in the field of L-lactic acid production, can solve the problem of low rate of acid production, achieve the effects of low probability of contaminating miscellaneous bacteria, reducing loss of nutrients, and reducing energy consumption

Active Publication Date: 2009-04-01
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patents USP7183088 B2 and CN 1498265 A mentioned that another strain of Bacillus coagulans (Bacillus coagulans SIM-7 DSM 14043) was used to produce L-lactic acid, but the rate of acid production was low. After 98 hours of fermentation, the acid production was 12.3%, and the conversion rate was 95.5% %

Method used

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  • Method for producing L-lactic acid and coagulate bacillus cereus special for the same
  • Method for producing L-lactic acid and coagulate bacillus cereus special for the same
  • Method for producing L-lactic acid and coagulate bacillus cereus special for the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1. Separation, mutagenesis screening and identification of Bacillus coagulans (Bacillus coagulans) CASH CGMCC №2184

[0035] 1. Separation and mutagenesis screening

[0036] The composition of the medium used in this embodiment is as follows:

[0037] Nutrient liquid medium: glucose 150 g / L, yeast powder 10 g / L, calcium carbonate 50 g / L, pH 6.

[0038]Nutrient agar medium: glucose 50 g / L, yeast powder 10 g / L, calcium carbonate 20 g / L, agar powder 10 g / L, pH 6.

[0039] Fermentation medium: glucose 150 g / L, yeast powder 20 g / L, calcium carbonate 90 g / L, pH 6.

[0040] Nutrient broth medium: glucose 50 g / L, yeast powder 10 g / L, calcium carbonate 10 g / L, pH 6.

[0041] The specific operation process of this embodiment is as follows:

[0042] Soil samples were obtained near a milk factory in Mentougou District, Beijing. Every 2 grams of soil samples were placed in 50ml of nutrient liquid medium, and enriched at 55°C for 6-10 hours. Then it was diluted and appli...

Embodiment 2

[0047] Embodiment 2, Utilize Bacillus coagulans (Bacillus coagulans) CASH CGMCC №2184 to produce L-lactic acid by fermentation in Erlenmeyer flask

[0048] The composition of the medium used in this embodiment is as follows:

[0049] Slant medium: glucose 20 g / L, yeast extract 5 g / L, peptone 10 g / L, beef extract 10 g / L, magnesium sulfate heptahydrate 0.58 g / L, manganese sulfate tetrahydrate 0.25 g / L, carbonic acid Calcium 15 g / L, agar powder 15 g / L. The initial pH of the medium was 6.5. Sterilization was performed at 115°C for 20 minutes.

[0050] Seed medium: glucose 70 g / L, yeast powder 10 g / L, soybean peptone 5 g / L, calcium carbonate 30 g / L. The initial pH of the medium was 6.5. Sterilization was carried out at 115° C. for 20 minutes.

[0051] Fermentation medium: glucose (Zibo Hongyu Industrial Sugar Co., Ltd.) 170 grams / liter, yeast powder (Beijing Aoboxing Biotechnology Co., Ltd., catalog number 01-013) 15 grams / liter, calcium carbonate 80 grams / liter. The initial pH...

Embodiment 3

[0063] Example 3, Utilize Bacillus coagulans (Bacillus coagulans) CASH CGMCC №2184 to produce L-lactic acid by fermentation in Erlenmeyer flask

[0064] The culture temperature of Bacillus coagulans (Bacillus coagulans) CASH CGMCC №2184 is 55°C. The composition of the fermentation medium is: glucose (Zibo Hongyu Industrial Sugar Co., Ltd.) 118 grams per liter, soybean peptone (Beijing Aoboxing Biotechnology Co., Ltd., catalog number 01-004) 15 grams per liter, calcium carbonate 80 grams / Lift. The initial pH of the fermentation medium was 5.5. The fermentation time is 24 hours. Other methods are all the same as in Example 2.

[0065] At the end of the fermentation, the concentration of glucose was 3.2 g / L ± 1.1 g / L (mean ± standard deviation), the concentration of L-lactic acid was 113.5 g / L ± 1.9 g / L (mean ± standard deviation), and the sugar-acid conversion rate was 98.9 % ± 0.3% (mean ± standard deviation), volume production efficiency 4.7 g / L / h ± 0.2 g / L / h (mean ± stan...

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Abstract

The invention discloses a production method of L-lactic acid and the special condensate bacillus CASH CGMCC No. 2184. L-lactic acid can be gotten by cultivating the special condensate bacillus CASH CGMCC No. 2184. Per litter of fermentation culture medium of the condensate bacillus comprises at least one of the following five nitrogen sources: yeast powder 5 to 15g, peptone 5 to 15g, soybean peptide 5 to 15g, soybean peptone 5 to 15g, and cottonseed protein 10 to 20g; in addition, the fermentation culture medium comprises glucose 80 to 170g (the content of initial glucose), calcium carbonate 50 to 100g and water; the pH of the fermentation culture medium is 5.5 to 7. With glucose as the substrate, under the condition of 45 to 60DEG C and with 98 to 99% of sugar acid conversion, the condensate bacillus CASH CGMCC No. 2184 efficiently ferments to produce L-lactic acid with optical purity of 99%; the concentration of the L-lactic acid maximum can attain 190 g / l and the volume production efficiency maximum can attain 5.7 g / l / h.

Description

technical field [0001] The invention relates to a method for producing L-lactic acid and the special-purpose Bacillus coagulans. Background technique [0002] Lactic acid, also known as dihydroxypropionic acid, is one of the three largest organic acids in the world and is widely used in food, cosmetic, pharmaceutical, textile and chemical industries. Polylactic acid made of lactic acid has good biocompatibility and biodegradability, and can be made into medical slow-release materials, biodegradable fibers and biodegradable plastics, etc., and is expected to replace petroleum-based plastics in the near future. In recent years, due to the increasing shortage of petroleum resources and the gradual enhancement of people's awareness of environmental protection, there are more and more researches on polylactic acid, and the demand for lactic acid as a monomer of polylactic acid is becoming more and more urgent. Lactic acid is a chiral molecule and can be divided into L-lactic aci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/56C12R1/07
Inventor 许平秦加阳赵博马翠卿于波
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI