A composition for wound healing and use thereof
A technology for wounds and uses, applied in drug combinations, medical preparations containing active ingredients, gene therapy, etc., can solve the problems of diabetic foot ulcers being easily infected and unable to be cured, etc.
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Embodiment 1
[0077] Example 1: Effect of TMD23 on migration of HaCaT epidermal cells
[0078] The effect of TMD23 on the migration of HaCaT cells was examined using a Boyden chamber with a 6.5 mm diameter polycarbonate filter membrane (8-μm pore size). Collagen type IV is coated on the surface of the lower filter membrane. Add TMD23 (100 ng / ml) or TMD23 (100 ng / ml) and anti-TM antibody (1 μg / ml) to DMEM in the lower well. Each upper well was added with 1 × 10 4 cell suspension (50 μL). Eight hours later, fixed with methanol and stained with 10% GIEMSA, the number of cells migrated through the membrane was counted under a microscope. Such as figure 1 As shown, TMD23 significantly induced the chemotactic migration of HaCaT cells.
Embodiment 2
[0079] Example 2: Materials
[0080] DMAp is 100 μg recombinant TMD23 purified protein (SEQ ID NO. 1 ) dissolved in 20 μl 0.5% CMC / PBS solution. 0.5% CMC (carboxymethylcellulose) / PBS (phosphate buffered saline) (pH 7.4) was used as a vehicle for DMAp and CGS-21680 solutions. CGS-21680 hydrochloride (2-p-[2-carboxymethyl]anilino-5'-N-ethylcarboxamidoadenosine), a G protein activator, was purchased from Sigma-Aldrich.
[0081] DMAc gel is 100 μg recombinant TMD23 purified protein (SEQ ID NO. 1 ) dissolved in 20 mg base gel. The basic gel above was used as a negative control for DMAc gel and Regranex gel. The above basic gel is composed of sodium carmellose, sodium chloride, sodium acetate trihydrate, glacial acetic acid, methylparaben, propylparaben, m-cresol 1-lysine hydrochloride, benzyl alcohol , methylchloroisothiazoline (methylchloroisothiazoline), methylisothiazoline (methylisothiazoline), ammonium acryloyldimethyltaurate / VP copolymer (ammoniumacryloyldimethyltaurate / ...
Embodiment 3
[0084] Example 3: Animals
[0085] CD-1 (Crl.) male mice weighing 24±2 g were provided by Taiwan BioLasco (licensed by Charles River Laboratories). A space of 29 x 18 x 13 cm was partitioned for 10 mice. All mice were kept in MDS Medical Service Company (Taiwan laboratory is preferred) for at least one week, maintaining a 12-hour light-dark cycle and controlled temperature (22°C-24°C) and humidity (50%-60%) environment. Standard laboratory mouse chow [MF-18 (Oriental Yeast Co., Ltd., Japan)] and RO water were freely available. Work including housing construction, experiments, and animal handling were performed in accordance with the Guide for the Care and Use of Laboratory Animals (National Academies Press, Washington D.C., 1996).
[0086] Non-insulin-dependent diabetic (NIDDM) male mice (C57BLKS / J-m+ / +Lepr db) weighing 50±5 g (9 weeks old) provided by the Institute of Animal Reproduction (IAR, Japan) were used. These mice all showed hyperinsulinemia, hyperglycemia and is...
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