Process of synthesizing gamma-D-glutamine acyl L-tryptophane by enzyme method

Abstract

The present invention relates to enzyme process of synthesizing gamma-D-glutamyl-L-tryptophane. Gamma-glutamyltranspeptidase is first added into the substrate solution comprising L-tryptophane in 8-50 mmol / L and gamma-D-glutamie in 5-35 mmol / L to produce the peptide-converting reaction preparing gamma-D-glutamyl-L-tryptophane in 8 hr, and the prepared gamma-D-glutamyl-L-tryptophane is then separated and purified. The present invention has simple process, low cost, less side reactions, high gamma-D-glutamyl-L-tryptophane converting rate, high yield and high product purity, and may be used in industrial production.

Description

technical field

[0001] The invention relates to an enzymatic synthesis method, in particular to a method for synthesizing γ-D-glutamyl-L-tryptophan by using γ-glutamyl transpeptidase. Background technique

[0002] The immune system is a network of cells adapted to protect an organism against pathogens and cells that are recognized as not "self". Once activated, the immune system recruits a wide variety of cells and components to enhance designated effector functions to eliminate "non-self" entities in the body. Lymphocytes are cells of the immune system capable of specifically recognizing and selectively eliminating foreign entities, as compared to other cells of the immune system, such as neutrophils, which are thought to be nonspecific in responding to invaders , Lymphocytes bring specificity, diversity, memory and self / non-self recognition to the immune response. There are two main groups of lymphocytes: B lymphocytes and T lymphocytes. The normal development, maturati...

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