Method for preparing polymer material with gradient distributed surface biomacromolecule

A technology for biomacromolecules and polymer materials, which is applied in the field of preparing polymer materials with gradient distribution of biomacromolecules on the surface, can solve the problems of long time consumption, many steps, poor gradient control effect, etc. Effects from a wide range of sources

Inactive Publication Date: 2007-09-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these preparation methods have disadvantages such as many steps, time-consuming, poor gradient control and insufficient stability, and are often not suitable for biomaterials with high biocompatibility requirements

Method used

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  • Method for preparing polymer material with gradient distributed surface biomacromolecule
  • Method for preparing polymer material with gradient distributed surface biomacromolecule
  • Method for preparing polymer material with gradient distributed surface biomacromolecule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) Dissolving hexamethylenediamine in n-propanol to form a homogeneous solution with a weight / volume concentration of 6%, as an aminolysis solution. The polylactic acid film was fixed vertically in a glass test tube with a diameter of 13.2 mm. The aminolysis solution was injected into the test tube with a micro-syringe at an injection rate of 0.4 ml / h, while the aminolysis temperature was controlled at 60° C. and the injection time was 10 minutes. The distribution curve of amino group concentration on the membrane surface after aminolysis is shown in Figure 1; the surface morphology is shown in Figure 2.

[0021] 2) After the polylactic acid film of gradient aminolysis was washed with three-distilled water, it was immersed in a glutaraldehyde solution with a mass concentration of 1% at room temperature for 3 hours, and then it was taken out and cleaned with three-distilled water to obtain a polylactic acid film with a gradient distribution of aldehyde groups on the surf...

Embodiment 2

[0024] 1) Dissolving hexamethylenediamine in n-propanol to form a homogeneous solution with a weight / volume concentration of 3%, as an aminolysis solution. The polylactic acid film was fixed vertically in a glass test tube with a diameter of 20 mm. The aminolysis solution was injected into the test tube with a micro-syringe at an injection speed of 5 ml / h, while the aminolysis temperature was controlled at 60° C., and the injection time was 10 minutes.

[0025] 2) After the polylactic acid film of gradient aminolysis was washed with three-distilled water, it was immersed in a glutaraldehyde solution with a mass concentration of 0.3% at room temperature for 6 hours, and then it was taken out and cleaned with three-distilled water to obtain a polylactic acid film with a gradient distribution of aldehyde groups on the surface. Lactic acid film.

[0026] Step 3) Same as in Example 1, obtaining a polylactic acid film with a surface collagen gradient immobilized.

Embodiment 3

[0028] Hexamethylenediamine was dissolved in n-propanol to form a homogeneous solution with a weight / volume concentration of 10%, which was used as an aminolysis solution. The polylactic acid film was fixed vertically in a glass test tube with a diameter of 13.2 mm. The aminolysis solution was injected into the test tube with a micro-syringe at an injection speed of 0.8 ml / h, while the aminolysis temperature was controlled at 50° C., and the injection time was 15 minutes.

[0029] Step 2) and step 3) are the same as in Example 1 to obtain a polylactic acid film with a collagen gradient immobilized on the surface.

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Abstract

The invention discloses a method of preparing polymer material with surface organism macromolecule of gradient distribution, which includes steps: putting polymer thin film with ester bond into container, continuously injecting amino-containing compound solution by injector, realizing gradient distribution of amino and eater bond reaction on polymer thin film surface, and obtaining polymer thin film of amino with gradient distribution surface; linking organism macromolecule and amino on polymer thin film surface by coupling agent, accordingly fixing the organism macromolecule on the polymer thin film surface and presenting the same gradient distribution. Advantages of the invention are: preparing method is simple, raw material is low-cost and facile, and the polymer material with gradient distribution surface is valuable for inducing gradient distribution of cell and leading growth of tissue.

Description

technical field [0001] The invention relates to a method for preparing a polymer material with gradient distribution of biomacromolecules on the surface. Background technique [0002] Regenerative medicine is an emerging discipline and tissue repair technology developed in recent years, involving cell differentiation and functional expression, design and preparation of bioactive materials, etc. Biomaterials play an important role in regenerative medicine. Biomaterials with appropriate structure and performance can maintain and enhance the growth of human cells and tissues, and realize the repair and replacement of tissues and organs. [0003] The tissues or organs of the human body are highly complex in terms of their composition, structure and function. Therefore, with the development of regenerative medicine, new challenges are posed to biomaterials. Designing and preparing biomaterials with cell regulation and induction functions on the surface to study the relationshi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/04C08J5/18C08L67/04C08L75/04C08L77/02A61L27/54
Inventor 高长有谈华平劳丽红
Owner ZHEJIANG UNIV
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