Fast breeding system for flame nandina

A flame Nandina, fast technology, applied in plant cells, horticulture, botanical equipment and methods, etc., can solve problems such as slow speed, difficult cutting, thin branches, etc., to improve economic benefits and meet market needs.

Inactive Publication Date: 2007-10-03
SHANGHAI ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

Since the Shanghai Botanical Garden introduced this species as a resource collection in 1995, no one has conducted systematic research on it. Due to the extremely short internodes, thin branches, cuttings are difficult and slow, the seedling breeding technology has not been improved. breakthrough
In 2002, Zhejiang Cixi Cotton Science Research Institute obtained the regenerated plants of Flaming Nandina through MS+6-benzylaminopurine 1.5mg / L+naphthalene acetic acid 0.5mg / L medium to induce adventitious buds, but the subculture required 45 days, and the proliferation of buds The coefficient is only 2.5 times, and there is no report on the rooting rate and the formation of large-scale rapid propagation technology regulations; in 2003, Shanghai Garden Research Institute and Shanghai Seed Industry Group also carried out research one after another, but the rooting rate was always hovering at 30%-50%. Nian Lu Youqin and others reported that Jiangsu Provincial Institute of Agriculture and Forestry Technology introduced and successfully cultivated flame nandina from abroad, but there was no specific report on seedling breeding and cultivation technology research that formed a certain number of scales in a short period of time.
This has limited the large-scale promotion of flame nandina to a certain extent, and it is difficult to realize the production value of high-quality seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Culture establishment

[0046] 1. Materials

[0047] The test materials came from France, a new species of ornamental tree species, Flaming Nandina.

[0048] 2. Method

[0049] (1) Cut off the branches of the year, and use a small brush to rinse the material under tap water. On the ultra-clean workbench, first place it in 70% ethanol for 30 seconds, and then rinse it with 0.1% HgCl 2 Soak for 5-7 minutes, then rinse with sterile distilled water 3-4 times.

[0050] (2) Under aseptic conditions, cut out 0.1-1.0 cm shoot tips or stem segments with buds and inoculate them on the starting medium.

[0051] (3) WP medium was used as the basic medium, 0.6% agar powder was solidified, the pH was 5.2, the carbon source was 2% commercially available white granulated sugar, the culture temperature was 25°C, and the light was 9-10 hours per day, and the light intensity was 1000lx.

[0052] (4) After 4 weeks, when the height of the new shoot reaches more than 1.0 cm, cut it into ...

Embodiment 2

[0054] proliferation of propagules

[0055] (1) The proliferation medium of the explant uses the WP basic medium and improves it: delete the copper sulfate (CuSO4) in the WP basic medium 4 .5H 2 O), adding zinc sulfate (ZnSO 4 .7H 2 O) dosage, additional 6-benzylaminopurine 1.0mg / L, the differentiation of flame Nandina stem section is better than mediums such as WH, B5 and MS.

[0056] (2) Proliferation culture once every 20 to 25 days, after 2 to 3 consecutive subcultures, use improved WP+6-benzylaminopurine 0.5mg / L+ indolebutyric acid 0.1mg / L for strong seedling culture to reduce For thin and weak seedlings, and so on, the two mediums are used alternately.

[0057] (3) The content of sugar in the improved WP medium was increased from 2% to 3% of the original WP medium, pH 5.8 instead of pH 5.2, and the culture conditions such as culture temperature, light time and light intensity were the same as above. After 30 days of culture, the reproduction coefficient of the expla...

Embodiment 3

[0060] root regeneration

[0061] (1) Induction start-up stage: according to the development degree of flame nandina xylem, select 1.4-1.6cm branches as cuttings and first culture them in WP "root differentiation medium" containing auxin 1 / 2 for 1-3 weeks. Plants treated with hormones were directly transplanted into the rooting medium as the control.

[0062] (2) Root elongation stage: transfer to hormone-free 1 / 2 improved WP "root development medium", which can effectively prevent the formation of callus, and avoid the problems of easy broken roots and low survival rate of seedlings during transplanting. After 3 weeks, the rooting frequency was counted.

[0063] (3) In the flame Nandina rooting medium, the carbon source concentration was reduced from the original 3% to 1.5%, and the wild Nandina was investigated to find that its root system grew well in weakly alkaline soil, so the pH was adjusted from 5.8 of the differentiation medium to 6.7 to 7.0 environment, the third s...

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Abstract

A fast reproduction system of flame nandina features that a controllable tissue culture technology is created, the culture medium for the efficient reproduction and strong plantlet culture is proportionally prepared from improved WP+6-benzylamino purine or improved WP+6-benzylamino purine, indolebutyric acid and granular white sugar, and the optimal rooting culture medium is proportionally from improved 1/2 WP and indolebutyric acid (or granular white sugar).

Description

technical field [0001] The invention relates to a plant breeding method, more specifically to a breeding system of an ornamental tree flame nandina. Background technique [0002] With the acceleration of the urbanization process, people are increasingly seeking for the richness and diversity of greening materials. From the original greening to beautification, from green to colorful, from flower viewing to scenery in all seasons. Flame Nandina domestica'Firepower', a new gardening variety of Nandina domestica bred abroad, belongs to the evergreen shrub of the genus Nandina in the family Berberidaceae. Compared with ordinary Nandina, the plant is dwarfed, the plant type is compact, and the leaves are oval or egg The shape is obviously different from the lanceolate leaves of the original species. After late autumn and winter, the emerald leaves gradually turn red, and in the middle of winter and early spring, they are like flames facing the wind, which is an important trait s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04A01G7/06
Inventor 杜永芹倪林娟王玉勤张永春刘贤雯
Owner SHANGHAI ACAD OF AGRI SCI
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