Method for producing epsilon-poly-L-lysine by reflux technique

A lysine and lysine fermentation technology, applied in the field of ε-poly-L-lysine production by reflux process, can solve environmental pollution and other problems, and achieve the effects of reducing environmental pollution, saving costs and reducing losses

Inactive Publication Date: 2007-11-28
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] In the existing ε-poly-L-lysine extraction process, the late penetration liquid and the late eluent are d

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example

[0018] What embodiment 1 and embodiment 2 basically describe is existing production technology;

Example

[0019] What embodiment 3 and embodiment 5 basically describe is the existing circulating production technology;

[0020] Example 4 and Example 6 are innovative processes compared with Example 3 and Example 5, respectively.

Example Embodiment

[0021] Example 1:

[0022] 5L fermenter, put into 2.7L culture medium, inoculate 300mL of Streptomyces albicans seed culture solution, cultivate at 30°C, automatically control to 30% when DO drops to 30%, cultivate in aerobic environment for 96h, and air flow rate of 3L / min~7.5 L / min. When the pH dropped to about 5.8, ammonia water (25-28%) was added to maintain the pH at 5.8 until the residual sugar concentration in the fermentation broth dropped to 10g / L, and the residual sugar concentration was maintained at about 10g / L by flow addition. The pH was no longer controlled to naturally drop to 4 and controlled at 4. Feed addition: glucose 800g / L, (NH 4 ) 2 SO 4 80g / L.

[0023] After culturing for 96h, the fermentation was completed, the cells and some solids were removed by centrifugation, the supernatant was eluted with D152 weakly acidic cation exchange resin, the early eluate was collected, filtered, concentrated in vacuo, and dried in a cyclone dryer to obtain ε- Poly...

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Abstract

This invention refer to a method that using backstreaming technology to produce epsilon -poly-L-diaminocaproic acid. It uses blanc Streptomyces that screened from soil to fermentepsilon -poly-L-diaminocaproic acid[resist to S-(2-aminoethyl)-L-cysteine(abbreviate AEC below), Glycine, sulfaguanidine],then make the penetrated liquid from later stage of extracted procession by periodic duty,to fermentepsilon -poly-L-diaminocaproic acid; the penetrated liquid from later stage of extracted procession can be used for next elution. Decolorize the broth by negion exchange resin. This invention has changed the procedure of producing technology.Not only raise the yield ofepsilon -poly-L-diaminocaproic acid, save cost, but also reduce exhaustion of ferment liquid waste and hydrochloric acid liquid waste in elution, lessen environmental pollution; The decolor effect is enhanced and the lose is reduced becouse of decoloring the broth by negion exchange resin.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for producing ε-poly-L-lysine by a reflux process. Background technique [0002] ε-Poly-L-lysine is a polypeptide containing 25-30 lysine residues secreted by Streptomyces albicans. It has good water solubility, easily soluble in water, slightly soluble in ethanol, but insoluble in ethyl acetate. , ether and other organic solvents; broad antibacterial spectrum, in acidic and slightly acidic environments, has inhibitory effect on Gram-positive bacteria, Gram-negative bacteria, the minimum inhibitory concentration is less than 100μg / mL, but for mold and yeast The inhibitory concentration of bacteria is higher, the minimum inhibitory concentration is lower than 250μg / mL, and it also has inhibitory effect on thermostable bacillus and some pathogens. Under certain conditions, some bacteriophages can be significantly inactivated; high thermal stability , ε-poly-L-lysine will not ...

Claims

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Application Information

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IPC IPC(8): C12P13/08C12N1/20C12R1/47
Inventor 贾士儒曹伟峰张恺瑞谭之磊孙艳芳李婷
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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