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Fish and shrimp phagostimulant of fermented product adhesion protein and its preparation process

A technology of fermented products and food attractants, which is applied in the processing of microorganism/unicellular algae protein, the molding or processing of animal feed, and animal feed, etc. , The effect of improving the intestinal flora of animals and promoting the healthy growth of animals

Inactive Publication Date: 2012-09-05
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It uses a special high-protein medium and fermented product. At the same time, high-temperature sterilization and microbial index testing are not carried out during the fermentation process, so it is difficult to guarantee the safety of the animals produced.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Step 1: Isolation, purification and preparation of beneficial bacteria

[0036] a. Isolation: In the intestinal tract of healthy crucian carp, the chyme was taken as the bacterial source, and the MRS lactic acid bacteria medium was used to separate the strains, and two kinds of lactic acid bacteria were isolated. There are two kinds of lactic acid bacteria used in this product, which are: Lactobacillus casei and licheniformis (13123), Bifidobacterium (Bifidobacterium ATCC7469). Isolation of Pseudomonas pseudomonas in the intestinal tract of prawns using yeast extract malt juice agar medium (Candidautilis CBCC5638);

[0037] b. Purification: the isolated Lactobacillus casei, licheniformis, and Bifidobacterium (Bifidobacterium) are marked and separated using a lactic acid bacteria medium, and Candida utilis is marked using a yeast extract wort agar medium Line and separation, the isolated strains are required to have obvious colony characteristics and neat colony shape; ...

Embodiment 2

[0054] The first step: with embodiment 1;

[0055] The second step: liquid culture of bacteria

[0056] a. Take by weighing the various strains produced in step 1 for seed production according to the following weight ratio: 40% of Lactobacillus casei, 40% of Bifidobacterium and 20% of Candida pseudomonas;

[0057] b. Put the weighed Lactobacillus casei, Bifidobacterium, and Candida pseudomonas into the liquid compound medium sterilized at 121°C for 40 minutes in a ratio of 1:33, and put them into a fermenter to stir for 30 minutes. 200r / min, after stirring evenly, put it into a 100L sterilized plastic bucket and let it stand for 13 days. During the standstill, measure the Ph value every day, and when the Ph reaches 3.0-4.0, the fermentation ends;

[0058] The weight percentage of the compound medium is as follows: 5% of soybean meal powder, 15% of molasses, 5% of yeast powder, 0.5% of potassium dihydrogen phosphate, 0.8% of sodium dihydrogen phosphate, 0.1% of magnesium sulfa...

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Abstract

The fish and shrimp phagostimulant of fermented product and adsorbed protein is prepared through screening beneficial bacteria seed from cultivated animal body, culturing in composite culture medium to proliferate, concentrating and adding plant protein with phagostimulant effect. It is added into fish and shrimp feed to raise their feed intake and improve their digesting function. The productionprocess has combined beneficial microbe fermenting technology and low temperature drying technology to ensure the fast growth of beneficial bacteria, the well preservation of metabolic product and the special cultivating function. The fish and shrimp phagostimulant is one kind of green product capable of replacing traditional phagostimulant and antioxidant.

Description

technical field [0001] The invention relates to a fish and shrimp food attractant produced by adsorbing protein from fermentation products and a preparation method thereof. By adding it to fish feed, the special fragrance and beneficial bacteria produced by microbial fermentation can be used to improve the intestinal tract and digestive function of aquatic animals, and improve the The feed intake and daily weight gain of animals belong to the technical field of agricultural biological products. Background technique [0002] Now in aquaculture, in order to pursue the fastest breeding effect and improve the storage time of feed, a large amount of antioxidants such as amino acid, betaine and ethoxyquinoline are added to the feed, which objectively achieves the goal of increasing the breeding output and attracting food. effect, but at the same time it makes the feed produce a strong odor, and betaine and antioxidants are currently chemically produced, and they are enriched in th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/16A23J3/20A23K10/18A23K20/147A23K40/10A23K50/80
CPCY02A40/818
Inventor 江瀚
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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