Technique for producing protein peptide and isoflavone extracted from bean sprout

A production process, soybean protein peptide technology, applied in the direction of fermentation, etc., can solve problems such as restricting the application of production process, and achieve the effects of good dispersibility, high water solubility, and reduction of waste water discharge.

Inactive Publication Date: 2008-01-16
DUNHUA CITY HUALI FOREIGN ECONOMIC & TRADE
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in the practical application of the above-mentioned technology, it is quite diffic

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0044] Example 1 (Soy Protein Isolate)

[0045] 1. Refining: The ratio of germinated soybean material to water is 1:4, and the slurry is refined by a refiner with 80 meshes and filtered into a slurry;

[0046] 2. Filtration: filter the above slurry with 100-120 mesh to remove the filter residue for use;

[0047] 3. Acid precipitation: use 20% edible hydrochloric acid or citric acid, phosphoric acid: adjust PH=4.5, stir for 30 rpm, and settle for 30 minutes;

[0048] 4. Centrifugal separation: the above-mentioned centrifugal dehydration is carried out to separate the whey solution and protein precipitate;

[0049] 5. Wash the above-mentioned protein precipitate with 60℃ warm water and centrifuge, each for two times;

[0050] 6. Water purification callback: add NaOH above to adjust PH=6~7, and heat at 90℃ for 10 minutes;

[0051] 7. Decolorization and desalination: Decolorization of the protein precipitate by activated carbon and reverse osmosis desalination;

[0052] 8. Sterilizati...

Example Embodiment

[0055] Example 2 (Soy protein peptide)

[0056] 1. According to the step 1 of Example 1, heat to 80°C and adjust the PH=8 by adding NaOH;

[0057] 2. Hydrolyze the above with alkaline protease, control the temperature at 65~70℃, adjust the PH=7~8, and the time is 6 hours;

[0058]3. Enter the above-mentioned enzymatic hydrolysate into the ultrafilter for separation, and adopt double membrane treatment, the molecular weight cutoffs are respectively: 300-1000,300; if it is greater than 1000, return to continue hydrolysis;

[0059] 4. Add the enzymolysis solution with a molecular weight cutoff of 300-1000 to activated carbon for debittering and decolorization;

[0060] 5. After entering the above-mentioned enzymatic hydrolysate into the ion exchanger for desalination, it is subjected to 135℃ ultra-high temperature instantaneous sterilization;

[0061] 6. Concentrate and dry the above-mentioned enzymolysis solution in a vacuum to obtain the finished soybean protein peptide.

Example Embodiment

[0062] Example 3 (Soybean Oligosaccharide)

[0063] 1. Heat the whey solution separated in step 4 of Example 1 to 70°C for purification to remove residual protein;

[0064] 2. Desalting: Put the purified soybean whey solution into the desalting device to remove 95% of the salt;

[0065] 3. Resin adsorption: the soybean whey after demineralization enters the resin to absorb other components, improving the taste and flavor of the sugar, reducing the color value and decolorizing at the same time;

[0066] 4. Concentration: Reverse osmosis dehydration is used to make the solution sugar concentration above 12%. The dialysis water can be used for multiple times, and the sugar solution can be concentrated to 30% by vacuum evaporation after concentration;

[0067] 5. Purification: Enter 30% of the solution sugar into an ultrafiltration device for membrane separation, remove suspended solids and bacteria, and dry to obtain soybean oligosaccharides.

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Abstract

The invention discloses a production process of extraction soybean peptide and isoflavone from bean sprout, comprising refining, filtering, acid precipitation, centrifugal separation for protein solution, purified water call back, decolor, desalination, call back, enzymatic hydrolysis, ion exchange, drying concentration to get soybean peptide; desalination, resin adsorption, alcohol washing, dealcoholization, drying concentration to centrifugal separated whey solution to get soybean isoflavone. The invention adopts bean sprout as raw material and percolation technique by ultrafiltration membrane, which can not only save energy and recycle waste water, but also can realize cleaner production with discharge of waste water reduced.

Description

technical field [0001] The invention relates to a soybean deep processing technology, in particular to a production process for extracting protein peptides and isoflavones by processing soybean sprouts. Background technique [0002] As we all know, soybean has high-quality soybean protein, and soybean protein peptide, isoflavones, oligosaccharides, soybean fiber, soybean saponin, etc. can be extracted from soybean protein, and it has attracted people's attention. And has been widely used in medicine, food and health care and other fields. For this reason, in-depth research and development work has been carried out on this technical aspect. In this regard, there are also relevant reports, such as the Chinese invention patent, whose notification number is CN1687123A, which discloses a "membrane method for extracting soybean protein peptide and its by-product deep processing technology". The process is as follows: the low-temperature soybean meal is leached with lye, and acco...

Claims

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Application Information

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IPC IPC(8): C12P21/00C12P17/06
Inventor 徐勇李武唐晓伟翟玉春孙志勇
Owner DUNHUA CITY HUALI FOREIGN ECONOMIC & TRADE
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