Microorganism ferment purple sweet potato and preparation method thereof
A technology of microbial fermentation and purple sweet potato, applied in animal feed, animal feed, additional food elements, etc., can solve the problems of purple sweet potato being difficult to use, environmental pollution, resource waste, etc., achieve a wide range of use, increase immune function, and increase appetite Effect
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Embodiment 1
[0041] (1) First, culture the slant strains: each strain uses the corresponding medium and adds 1.5% agar, the inoculum size is 5%, cultures statically at 25°C for 20 hours, and puts them into a refrigerator at 4°C for storage. See Table 1 below for the strains used and the medium used for the strains:
[0042] Table 1
[0043] serial number
Bacteria name (Chinese, Latin)
serial number
culture medium
1
Bacillus
amyloliquefaciens
AS1.1226
Peptone 1%, beef extract 0.5%,
Sodium chloride 0.5%, pH7.2-7.4
2
IFFI10067
Peptone 1%, beef extract 0.5%,
Sodium chloride 0.5%, pH7.2-7.4
3
Lactobacillus acidophilus
acidophilus
IFFI06005
Skimmed Milk Powder 10%, pH 6.0
4
Candida utilis
Candida utilis
AS2.120
Peptone 2%, ...
Embodiment 2
[0050] (1) Firstly, culture the slant strains: each strain uses the corresponding culture medium and adds 2.5% agar, the inoculum size is 10%, cultures statically at 35°C for 48 hours, and puts them into a refrigerator at 4°C for storage. See Table 1 below for the strains used and the medium used for the strains:
[0051] Table 1
[0052] serial number
Bacteria name (Chinese, Latin)
serial number
culture medium
1
Bacillus
amyloliquefaciens
AS1.1226
Peptone 1%, beef extract 0.5%,
Sodium chloride 0.5%, pH7.2-7.4
2
Bacillus coagulans
IFFI10067
Peptone 1%, beef extract 0.5%,
Sodium chloride 0.5%, pH7.2-7.4
3
Lactobacillus acidophilus
Lactobacillus
acidophilus
IFFI06005
Skimmed Milk Powder 10%, pH 6.0
4
Candida utilis
Candida utilis
AS2.120
P...
Embodiment 3
[0059] (1) Firstly, culture the slant strains: each strain uses the corresponding culture medium and adds 2% agar, the inoculum size is 8%, cultures statically at 30°C for 40 hours, and puts them into a refrigerator at 4°C for storage. See Table 1 below for the strains used and the medium used for the strains:
[0060] Table 1
[0061] serial number
Bacteria name (Chinese, Latin)
serial number
Incline medium
1
Bacillus
amyloliquefaciens
AS1.1226
Peptone 1%, beef extract 0.5%,
Sodium chloride 0.5%, pH7.4
2
Bacillus coagulans
Bacillus coagulans
IFFI10067
Peptone 1%, beef extract 0.5%,
Sodium chloride 0.5%, pH7.2
3
Lactobacillus
acidophilus
IFFI06005
Skimmed Milk Powder 10%, pH 6.0
4
Candida utilis
Candida utilis
AS2.120
Peptone 2%, ...
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