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Method for simultaneously determining multiple polyphenolic compound content of fruit

A polyphenolic compound, a variety of polyphenol technology, applied in measuring devices, instruments, scientific instruments and other directions, can solve the problems of difficult and accurate measurement, complex methods, low polyphenol content, etc., to achieve improved measurement accuracy and recovery rate, Column protection, high accuracy effect

Inactive Publication Date: 2010-12-15
FUDAN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the types of polyphenols currently studied are quite different, and they are limited to a small number of fruits such as apples, grapes, and citrus.
In addition, the juicing method is usually used to prepare fruit samples. Although the juicing method is very simple, it is difficult to measure accurately because the water content in the sample is too high and the polyphenol content is too low, and the extraction efficiency of some polyphenols is low.
Many fruit juices cannot be directly used for measurement, and the lipids, carotenoids and chlorophyll contained in them need to be removed, and the polyphenols in some fruit juices need to be hydrolyzed before measurement, and the method is more complicated

Method used

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  • Method for simultaneously determining multiple polyphenolic compound content of fruit
  • Method for simultaneously determining multiple polyphenolic compound content of fruit
  • Method for simultaneously determining multiple polyphenolic compound content of fruit

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Embodiment Construction

[0028] The present invention will be further described below in conjunction with examples.

[0029] 1. Preparation of reference solution:

[0030] Standard samples of gallic acid, protocatechin, catechin, epicatechin, caffeic acid, rutin, chlorogenic acid, and ferulic acid with purity ≥99% were taken. Prepare a standard stock solution of about 1g / L of the above 8 polyphenols with 10% methanol solution. According to the actual content of polyphenols in the fruit and the response value of each polyphenol at a certain UV detection wavelength, diluted a certain multiple to prepare a mixed standard solution containing the above 8 polyphenol compounds, and then dilute the above solution to different concentration gradients , That is, 0.2, 0.4, 0.6, 0.8, 1.0 times the concentration of the above-mentioned mixed standard solution.

[0031] 2. Preparation of test solution:

[0032] Sample preparation by microwave extraction method: Fresh fruit samples (Hebei Snow Pear, Hebei Snow Pear Peel, F...

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Abstract

The utility model discloses a high efficiency liquid phase chromatogram method which can detect the content of various polyphenolic compounds inside fruits at the same time by adopting liquid phase chromatogram containing ultraviolet detector and reversed-phase bonded silica chromatographic column; organic solvent mobile phase A and acidic aqueous mobile phase B with certain Ph value are adopted as gradient eluant. The utility model quantitatively detects various polyphenolic compounds inside fruits at the same time only on normal high efficiency liquid phase chromatogram. The utility model builds a method of detecting the content of various polyphenolic compounds inside fruits which combines microwave extraction and high efficiency liquid phase chromatogram. The utility model has the advantages of advanced and accurate method, simple and quick sample processing; meanwhile, Carrez reagent is used as scavenging agent of fruits sample and is firstly used as fruit polyphenols testing.

Description

Technical field [0001] The present invention relates to a method for determining the content of fruit polyphenol compounds. Using a common high-performance liquid chromatograph and specific chromatographic conditions, it is possible to complete the gallic acid, protocatechin, catechin, chlorogenic acid and epithelial The determination of polyphenolic compounds such as tea, caffeic acid, ferulic acid, or rutin yields accurate results. Background technique [0002] Fruit polyphenols have medicinal and edible value. Due to the unique chemical and biological properties of polyphenols, polyphenols are extracted from edible plants and purified. They can be used as natural food additives to regulate food flavors and act as antioxidants ( Efficient, non-toxic and health-care) and antiseptic effect. The polyphenolic compounds in fruits not only have medicinal and edible value, but also one of the important indicators of fruit maturity. Commonly used methods for the determination of phen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/89
Inventor 张晋芬袁冰徐华龙沈伟
Owner FUDAN UNIV
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