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Preparation method of 17beta-estradiol molecular engram polymer and use

A molecular imprinting and polymer technology, applied in material separation, analysis materials, instruments, etc., can solve the problems of expensive investment, impossible to comprehensively promote, unsatisfactory reproducibility and accuracy, etc.

Inactive Publication Date: 2008-03-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, commonly used steroid detection techniques include fluorescence spectroscopy (Van Ginkel G., Van Langen H.et al.1989), mass spectrometry (Palmgrén J.J., Tyrs A.et al.2005), liquid chromatography Chromatography (Grob K., Lanfranchi M. et al. 1989), gas chromatography (Truong T.T., Marriott P.J. et al. 2003), thin layer chromatography (Pazos A.J., Silva A. et al. 2003), immunoassay (Abraham G.E. 1975) and some combinatorial analytical techniques, most of which require expensive investment, cumbersome sample pretreatment procedures and extensive use of organic solvents, except for immunoassays
Although immunoassay is a very suitable method for analyzing endogenous hormones, there are also problems of specificity and sensitivity in practical applications, and it also relies on a large number of animal experiments, which are limited by conditions, such as high experimental costs, making it impossible Comprehensive promotion, but time-consuming and labor-intensive, reproducibility and accuracy are not ideal

Method used

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  • Preparation method of 17beta-estradiol molecular engram polymer and use
  • Preparation method of 17beta-estradiol molecular engram polymer and use

Examples

Experimental program
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Effect test

Embodiment 1

[0036] According to the following proportions, such as 0.125mmol, 0.167mmol and 0.25mmol of 17β-estradiol as the template molecule, 1mmol of methyl methacrylate as the functional monomer, and 1mmol of trimethylolpropane trimethacrylate as the crosslinker 20mL chloroform is used as porogen, 0.102mmol azobisisobutyronitrile is mixed and dissolved, firstly methyl methacrylate, 17β-estradiol and 10mL acetonitrile are added to a 100mL medical saline bottle for ultrasonic treatment and mixing, washed into Seal with nitrogen for 3 minutes, and leave it at room temperature for more than 3 hours; then add the above mixture to the crosslinking agent 1mmol trimethylolpropane trimethacrylate, the remaining 5mL of porogen and initiator, mix well, and then flush with nitrogen for 3 minutes to seal. Precipitation polymerization is carried out in a vessel using thermal or photoinitiated means. The thermal initiation reaction condition is that the polymerization temperature is controlled at a ...

Embodiment 2

[0045] Use 0.125mmol and 0.25mmol 17β-estradiol as template molecule, 1mmol trifluoromethacrylic acid as functional monomer, 1mmol trimethylolpropane trimethacrylate as crosslinking agent, and 15mL acetonitrile as Pore ​​agent, 0.0852mmol of azobisisobutyronitrile is mixed and dissolved, respectively added to 100mL medical saline bottle, ultrasonically treated for 3min, and N 25min, seal. Utilize 35W ultraviolet lamp to irradiate at 365nm for 48h, ultraviolet polymerization UV 365 nm control at 0 ~ 8 ℃, centrifugal separation. The synthesis method of CP is the same, but no template molecule is added.

[0046] The obtained polymer was transferred to a round bottom flask, mixed with 40mL of acetone twice, and the fine particles in the upper layer were removed after standing, and then the acetone was removed in vacuum with a rotary evaporator to dryness. Pack the polymer into the chromatographic column (4.6×100mm) by hand, and wash it with a Waters model 510 high-efficiency li...

Embodiment 3

[0060] 0.125mmol and 0.25mmol of 17β-estradiol were used as the template molecule, 1mmol of trifluoromethacrylic acid as the functional monomer, 1mmol of trimethylolpropane trimethacrylate as the cross-linking agent, and 15mL of acetonitrile as the porogen agent, 0.0852mmol azobisisobutyronitrile was mixed and dissolved, respectively added to 100mL medical saline bottle, treated with ultrasonic wave for 3min, and passed through N 2 5min, seal. Utilize 35W ultraviolet lamp to irradiate at 365nm for 48h, ultraviolet polymerization UV 365 nm control at 0 ~ 8 ℃, centrifugal separation. The synthesis method of CP is the same, but no template molecule is added.

[0061] The obtained polymer was transferred to a round bottom flask, mixed with 40mL of acetone twice, and the fine particles in the upper layer were removed after standing, and then the acetone was removed in vacuum with a rotary evaporator to dryness. Pack the polymer into the chromatographic column (4.6×100mm) by hand...

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PUM

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Abstract

The present invention relates to the preparation method and the application of a 17 beta-estradiol molecularly-imprinted polymer, which belongs to the analysis and measurement technology field of the 17 beta-estradiol of the biological samples and food samples. The present invention proposes a method for the preparation of 17 beta-estradiol molecularly-imprinted polymer as well as the separation, purifying and examination method of the 17 beta-estradiol molecularly-imprinted polymer in the biological samples and food samples. The 17 beta-estradiol molecularly-imprinted polymer generated by the present invention is of selective absorption. The present invention can separate, purify, concentrate and fast examine and analyze the 17 beta-estradiol molecularly-imprinted polymers in the environmental, biological samples and food samples.

Description

technical field [0001] A preparation method and application of a 17β-estradiol molecularly imprinted polymer. The present invention relates to a preparation method for a polymer with specific recognition performance for 17β-estradiol, and also relates to the separation and purification of molecularly imprinted polymers and Application to the determination of 17β-estradiol in biological and food samples. The invention belongs to the technical field of analysis and determination of 17β-estradiol in biological samples and food samples. Background technique [0002] Molecular imprinting is the preparation of polymers with molecular recognition properties. It is regarded as a specific new affinity technology. As a tool for exploring intermolecular interactions, it belongs to the category of host-guest chemistry in supramolecular chemistry. Molecularly imprinted polymer (MIP) is based on the "tailor-made" target molecule (Mosbach K. and Haupt K.1998), and has the reputation of "m...

Claims

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Application Information

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IPC IPC(8): C08F220/00C08F2/44C08J9/04C08J9/26G01N30/50
Inventor 汤坚朱秋劲顾小红
Owner JIANGNAN UNIV
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