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Production method of solid preparation for degradation of ammonia nitrogen and nitrite

A nitrite and ammonia nitrogen technology, applied in chemical instruments and methods, biochemical equipment and methods, enzymes, etc., can solve environmental problems and achieve the effects of reducing ammonia nitrogen and nitrite nitrogen content, complete degradation, and simple operation.

Inactive Publication Date: 2008-04-09
江苏绿科生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term and extensive use of antibiotics and other chemical drugs has caused a series of environmental and social problems

Method used

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  • Production method of solid preparation for degradation of ammonia nitrogen and nitrite
  • Production method of solid preparation for degradation of ammonia nitrogen and nitrite
  • Production method of solid preparation for degradation of ammonia nitrogen and nitrite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] 1. Test plan

[0011] 1. Preparation of composite microorganism and composite enzyme solid preparation

[0012] Take by weight 1% molasses and 0.07% corn steep liquor freeze-dried powder as basic medium, add 0.001% ammonium sulfate again; After dissolving ammonium sulfate, molasses and corn steep liquor freeze-dried powder with water, join in the fermentation tank, add The liquid volume is 70% of the fermenter. After autoclaving for 30 minutes, inoculate after cooling to 33°C, cultivate Bacillus subtilis (37°C), nitrifying bacteria (37°C), photosynthetic bacteria (35°C), denitrifying bacteria (35°C) at different temperatures , saccharomycetes (30°C), actinomycetes (30°C) and other six strains, cultivated for 20 hours, centrifuged the culture solution, collected the thalline, added bran 3 times the weight of the thalline to the thalline, and The mixture of thalline and bran is boiled and dried in a fluidized bed to obtain the dry bacterial powder of the composite micro...

Embodiment 2

[0025] 1. Test plan

[0026] 1. Preparation of composite microorganism and composite enzyme solid preparation

[0027] Take by weight 1.2% molasses and 0.06% corn steep liquor freeze-dried powder as basic medium, add 0.0015% ammonium sulfate again; After dissolving ammonium sulfate, molasses and corn steep liquor freeze-dried powder with water, join in the fermentation tank, add The liquid volume is 70% of the fermenter. Autoclave for 30 minutes, inoculate after cooling to 33°C, cultivate Bacillus subtilis (37°C), nitrifying bacteria (37°C), photosynthetic bacteria (35°C), denitrifying bacteria (35°C) and Six strains of yeast (30°C) and actinomycetes (30°C) were cultured for 22 hours, the culture solution was centrifuged to collect the bacteria, and bran twice the weight of the bacteria was added to the bacteria. The mixture of thalline and bran is boiled and dried in a fluidized bed to obtain a composite microbial dry bacterial powder, then add protease, lipase and amylase ...

Embodiment 3

[0039] 1. Test plan

[0040] 1. Preparation of composite microorganism and composite enzyme solid preparation

[0041]Take by weight 0.9% molasses and 0.09% corn steep liquor freeze-dried powder as basic medium, add 0.0012% ammonium sulfate again; After dissolving ammonium sulfate, molasses and corn steep liquor freeze-dried powder with water, join in the fermentation tank, add The liquid volume is 70% of the fermenter. Autoclave for 30 minutes, inoculate after cooling to 33°C, cultivate Bacillus subtilis (37°C), nitrifying bacteria (37°C), photosynthetic bacteria (35°C), denitrifying bacteria (35°C) and Six strains of yeast (30°C) and actinomycetes (30°C) were cultured for 24 hours, the culture solution was centrifuged to collect the bacteria, and bran four times the weight of the bacteria was added to the bacteria, and the The mixture of thalline and bran is boiled and dried in a fluidized bed to obtain a composite microbial dry bacterial powder, and then protease, lipase ...

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Abstract

The invention discloses a preparation method of compound microorganisms and a recombination enzyme solid agent which can degrade the ammonia- nitrogen and nitrite in the aquicultural water environment. The invention uses universal liquid medium to culture bacillus subtilis, nitrifying bacteria, photosynthetic bacteria, denitrifying bacteria, yeast, actinomyces etc six under different temperatures. Then the culture liquid is centrifugally separated and then the bacteria body is collected. The bran is added into the bacteria body as an adsorbent. The mixture of the bacteria body and the bran is boiled and dried on a fluidized bed. And the freeze-dried powder of compound microorganisms is obtained. Then proteases, lipase, and amylase are added to make the compound microorganisms and the recombination enzyme solid agent. The invention is put into the aquicultural water that needs to be dealt with and becomes nitro-nitrogen by the decomposition of proteases, lipase, and amylase and the ammonification of bacillus subtilis. Next, the nitro-nitrogen becomes nitrogen which is released into the air. So the invention can take effect quickly on completely degrading the ammonia- nitrogen and nitrite in the aquicultural water.

Description

technical field [0001] The present invention relates to a method for preparing a microbial modifier for aquaculture water environment, in particular to a method for preparing a composite microorganism and a composite enzyme solid preparation that degrades ammonia nitrogen and nitrite in aquaculture water environment Background technique [0002] China is the world's largest aquaculture country in terms of aquaculture output and aquaculture scale, but China's aquaculture technology level is relatively low. The goal is that the breeding density is too high, and the awareness of protecting the breeding environment is weak, and the breeding diseases are increasing year by year, causing great economic losses. At present, most of my country's aquaculture industry is static water aquaculture. High-density aquaculture causes a large amount of organic matter such as fish and shrimp excrement and residual bait to deposit on the bottom of the water, making the oxide layer on the surfac...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N9/98C12N1/20C12N1/16C02F3/00C02F3/34A01K63/04
Inventor 路怀灯董英张红印徐斌
Owner 江苏绿科生物技术有限公司
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