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Method for purifying esgoside and extracting biological active compound

A biologically active substance, the technology of mogroside, which is applied in the direction of steroids, organic chemistry, and pharmaceutical formulations, can solve the problems that no one has proposed to purify mogroside

Inactive Publication Date: 2008-04-30
湖北汇特生物医药技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, no one has proposed a method for purifying mogroside and simultaneously extracting antimicrobial active substances

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: For Luo Han Guo

[0019] Mechanically pulverize 10g of Luo Han Guo fruit to obtain Luo Han Guo coarse powder, or crush 10g of fresh Luo Han fruit to obtain wet pieces, extract with 100ml of ethyl acetate for more than 120min to obtain an organic layer; repeat the extraction of the organic layer 3 times , separating the organic layer and the coarse powder of Luo Han Guo; combining the organic layers, drying it to obtain about 0.4g "mogroside"; spray drying to obtain about 9.4g of relatively pure mogroside.

Embodiment 2

[0020] Embodiment 2: For Luo Han Guo

[0021] Mechanically pulverize 100g of Luo Han Guo dried fruit to obtain coarse powder, or crush fresh 100g of Luo Han Guo to obtain wet pieces, soak the coarse powder and crushed pieces with 500ml of water, or 50% ethanol aqueous solution, or 50% methanol aqueous solution block, and heated to at least 80°C, and extracted for at least 60 minutes to obtain an extract liquid; the extraction was repeated 3 times, and the extract was filtered to obtain 1200ml of filtrate; the ethanol was evaporated to obtain an aqueous solution. Extract at least once with a non-polar solvent such as n-hexane to remove fatty acids, proteins and some other impurities; separate the organic layer and the aqueous layer, and keep the aqueous layer. Extract the aqueous layer with 1000ml of moderately polar solvent ethyl acetate for at least 3 times to obtain an organic layer. This organic layer contains "mogroside", while the aqueous layer contains mogroside; g"Mang...

Embodiment 3

[0022] Embodiment 3: For Luo Han Guo

[0023] Mechanically pulverize 100g of dried Luo Han Guo fruit to obtain coarse powder, or crush fresh Luo Han Guo fruit to obtain wet fragments, and use 500ml of water or 50% ethanol aqueous solution to heat to at least 80°C for more than 60 minutes to obtain Extract liquid; centrifuge and keep the supernatant and solid, repeat the extraction of solid matter 3 times, filter the Luo Han Guo extract to obtain the filtrate; evaporate the ethanol to obtain the aqueous solution; filter the extract to obtain about 1200ml of the filtrate; the filtrate has been passed through 100ml Water-balanced non-polar Amberlite XAD column; wash with 1200ml of water to remove unadsorbed substances; then use 300ml of 10% to 95% ethanol for gradient elution, 40% to 60% of the eluent contains Luo Han Guo Glycosides, respectively collect 40% to 95% of the eluate, combine and dry 40% to 70% of the eluate to remove ethanol to obtain an aqueous solution of Luo Han G...

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PUM

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Abstract

The invention discloses purification momordica grosvenori glucoside and a method for withdrawing the biological activity material, the method uses the solvent-extraction process to purify momordica grosvenori glucoside, simultaneously withdraws the momordica grosvenori element, and includes the steps that the fluid is prepared, the extract is carried on with moderate nonpolar resolver, the withdrawing is carried on with medium polar organic solvent, and the spraying and the drying are performed. The method of the invention can eliminate the return bitter taste in the momordica grosvenori, the momordica grosvenori sweet material, the extraction of the momordica grosvenori, the momordica grosvenori concentration fruit juice, the momordica grosvenori sweetening agent, and the product, thereby improving momordica grosvenori sweet material taste, purifying momordica grosvenori glucoside, and simultaneously withdrawing the active material, namely, the momordica grosvenori element having anti-microorganism. The momordica grosvenori element has the activeness suppressing microorganism, such as Gram-negative bacteria, Gelanshi masculine fungus and fungus and so on. The anti-microorganism activeness of the momordica grosvenori element extracted with the invention can be used to prepare the anti-microorganism medicine and daily necessities like cough syrup, throat piece, toothpaste, rinses mouth fluid and so on, and the invention can have very great use value and economic value.

Description

technical field [0001] The present invention relates to the purification of natural condiment, in particular, to the method of purifying mogroside and simultaneously extracting biologically active substances. Background technique [0002] Luo Han Guo is the fruit of the perennial root climbing vine of Cucurbitaceae (Siraitiagrosvenorii). Luo Han Guo generally refers to the fruit of the plant (Siraitia grosvenorii), but also includes related similar plant species: S. siamensis, S. silomaradjae, S. sikkimensis, S. siraitia, S. borneensis and S. taiwaniana. Mogroside, the sweet component in Luo Han Guo, is a triterpenoid compound whose glucose ring is connected to the mogrol group. Luo Han Guo extract is a powder with a mogroside content of 60% to 99%, and has been widely used as a sweetener at present. However, currently produced Luo Han Guo extracts still contain a certain degree of aftertaste, which affects the mouthfeel of Luo Han Guo extracts and limits its application a...

Claims

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Application Information

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IPC IPC(8): C07J9/00C07G17/00A61K36/42A61K131/00C07G99/00
Inventor 周英黄赤夫
Owner 湖北汇特生物医药技术有限公司
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