Monoclonal antibody IgM type RhD blood type shaped reagent

Abstract

The invention provides an RhD blood grouping reagent with a monoclonal antibody IgM type, which consists of anti-D monoclonal cell culture concentrated solution and dilution solution, the anti-D monoclonal cell culture concentrated solution is prepared by using the following method, firstly, the invention includes an establishing process of a hybrid cell system, the establishing process of the hybrid cell system includes a process of making use of lymph cells transformed by EB virus to hybridize with the mouse bone marrow cells, the hybrid monoclonal cell system is established by the process, secondly, the invention includes a culture process of the hybrid monoclonal cell system, the culture solution of the monoclonal cells is collected to carry out the concentration by the culture of the hybrid monoclonal cell system, so as to obtain the anti-D monoclonal cell culture concentrated solution; the dilution solution consists of sodium chloride, sodium phosphate with 12 water molecules, potassium dihydrogen phosphate, stabilizer, sodium azide, Tween 20 and distilled water; as the monoclonal antibody is adopted, the invention has strong specific antibody response and high accuracy rate of the detection.

Description

Technical field: [0001] The invention relates to the field of biochemistry, in particular to a reagent for identifying blood types, specifically a monoclonal antibody IgM type RhD blood type typing reagent. Background technique: [0002] Generally, Rh blood types are divided into Rh positive and Rh negative according to the presence or absence of D antigen on the surface of red blood cells clinically. If an Rh-negative mother conceives an Rh-positive fetus, the mother will produce Rh antibodies, which can cause hemolytic disease of the newborn. Therefore, the detection of Rh positive is especially important for this disease. [0003] Most of the antibodies used clinically to identify human erythrocyte blood types are taken from human serum. In this way, not only a large amount of human serum is consumed, but also unimaginable consequences may be caused by some unknown sources of infection that may exist in human serum. Moreover, at present, polyclonal antibodies are often ...

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a RhD blood typing reagent of monoclonal antibody IgM type, and described monoclonal antibody IgM type RhD blood typing reagent will solve the problem in the prior art that the antibodies for identifying human red blood cell blood types are mostly taken from human serum, It needs to consume a large amount of human serum, and some unrecognized infectious sources that may exist in human serum may cause technical problems in human infection, and at the same time solve the technical problems of poor specificity of polyclonal antibody reaction and high misjudgment rate