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Macromolecule engram phosphoric acid/polyacrylic acid/calcium alginate multi-hybrid polymer micro-sphere and preparation method thereof

The technology of polyacrylic acid and calcium alginate is applied in the field of material science to achieve the effect of simple and easy preparation method, wide application prospect, and low mechanical strength and imprinting efficiency.

Inactive Publication Date: 2008-05-28
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the present invention, phosphate, polyacrylate and alginate are cross-linked with calcium ions to prepare macromolecularly imprinted multi-hybrid microspheres, and no relevant reports have been seen so far.

Method used

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  • Macromolecule engram phosphoric acid/polyacrylic acid/calcium alginate multi-hybrid polymer micro-sphere and preparation method thereof
  • Macromolecule engram phosphoric acid/polyacrylic acid/calcium alginate multi-hybrid polymer micro-sphere and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1. Preparation of bovine serum albumin surface imprinted phosphoric acid / polyacrylic acid / calcium alginate multi-hybrid polymer microspheres, comprising the following steps:

[0036] 1) Add 0.15g of sodium polyacrylate, 0.5128g of sodium alginate and 0.15g of diammonium hydrogen phosphate solid into 20ml of deionized water, stir to dissolve it fully, and obtain solution A;

[0037] 2) Measure 40ml of organic phase toluene, pour it into a beaker, add 0.8g sorbitan monooleate to it, stir for 5min to fully dissolve it; slowly pour solution A into the beaker containing the organic phase while stirring , stirred for 10 minutes to disperse the viscous solution A into microspheres;

[0038] 3) Add 0.1% bovine serum albumin with a mass percentage concentration of 0.1% and 20 ml of a calcium chloride mixed aqueous solution B with a mass percentage concentration of 5% to the above mixing system under stirring for gelation reaction, and continue stirring for 45 minutes ...

Embodiment 2

[0040] Example 2. The preparation of bovine serum albumin-embedded imprinted phosphoric acid / polyacrylic acid / calcium alginate multi-hybrid polymer microspheres comprises the following steps:

[0041] 1) Add 0.15 g of sodium polyacrylate, 0.5128 g of sodium alginate and 0.15 g of diammonium hydrogen phosphate solid to 0.1% bovine serum albumin solution dissolved in 20 ml of deionized water, and stir to fully dissolve it to obtain a solution A1;

[0042] 2) Measure 40ml of the organic phase composed of 20ml of chloroform and 20ml of n-hexane, pour it into a beaker, add 0.8g of sorbitan monooleate to it, stir for 5min to fully dissolve it; slowly dissolve the solution A1 under stirring Pour it into the beaker containing the organic phase, and stir for 15 minutes to disperse the viscous solution A1 into microspheres;

[0043] 3) Add 120ml of calcium chloride mixed aqueous solution B120ml with a mass percentage concentration of 5% to the above mixing system under stirring for gel...

Embodiment 3

[0045] Example 3. Preparation of phosphoric acid / calcium alginate hybrid polymer microspheres imprinted on the surface of ovalbumin, comprising the following steps:

[0046] 1) Add 0.15 g of sodium polyacrylate, 0.6186 g of sodium alginate and 0.15 g of diammonium hydrogen phosphate solid into 20 ml of deionized water, and stir to fully dissolve it to obtain solution A;

[0047] 2) Measure 40ml of the organic phase composed of 15ml butyl acetate and 25ml castor oil, pour it into a beaker, add 0.04g ethyl cellulose to it, stir for 5min to fully dissolve it; slowly pour solution A into In the beaker containing the organic phase, stir for 10-15 minutes to disperse the viscous solution A into microspheres;

[0048] 3) Add 10% ovalbumin by mass percentage concentration and 5% calcium chloride mixed aqueous solution B 20ml to the above mixing system under stirring to carry out gelation reaction, and continue stirring for 50 min to obtain microspheres; Using the method of patent 200...

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Abstract

The invention relates to a macromolecular engram of multiple hybridigation polymeric compound microsphere of phosphoric acid / polyacrylic acid / calcium alginate and process for preparation. The protein engram of multiple hybridigation polymeric compound microsphere of phosphoric acid or polyacrylic acid or calcium alginate comprises the following raw materials and parts by weight: 20%-40% of calcium alginate, 1%-10% of diammonium phosphate, 0.5%-5% of poly-sodium acrylate, 50%-65% of calcium chloride and 0.1%-10% of protein. The prepared multiple hybridigation polymeric compound microsphere of phosphoric acid or polyacrylic acid or calcium alginate is tested by protein multiple binding, the test result indicates that the multiple binding content of engram microsphere can reach at least 3 times of the multiple binding content of non-engram microsphere which protein is not added, the result of electric conductivity titration and infrared spectrum testing indicate that a model multiple hybridigation component is produced under the joint action of diammonium phosphate, calcium alginate and CaCl2. The invention is simple and easy in the process for preparation and easy controllable in process of reaction and the like, the invention solves the shortcomings of low in mechanical strength and engram efficiency of the pure calcium alginate and astable in property and the like, thereby the applying foreground is wide.

Description

technical field [0001] The invention belongs to the technical field of material science, and in particular relates to a macromolecular imprinted phosphoric acid / polyacrylic acid / calcium alginate multi-hybrid polymer microsphere and a preparation method thereof. Background technique [0002] Due to the characteristics of some macromolecules such as proteins, such as large size, easy denaturation due to environmental influences, complex chemical structure, and unclear recognition mechanism, the research on large molecule western imprinting is far less systematic and in-depth than small molecule imprinting research. Proteins are generally non-rigid biomacromolecules, and their chemical structures are also very complex. It is difficult to generate definite recognition points in imprinted polymers by relying on several binding sites. Traditionally prepared molecularly imprinted polymers are highly cross-linked and stiff materials that do not have sufficient compliance and interna...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/26C08J3/12C08L5/04C08K3/32C08L33/02C08L89/00C08K3/14
Inventor 成国祥赵孔银张立广
Owner TIANJIN UNIV
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