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Method of extracting natural oxidationresistant active substanceoil from orange tree bark

A technology of natural antioxidants and active substances, applied in the directions of antitoxins, drug combinations, pharmaceutical formulations, etc., can solve the problems that natural antioxidants have not been reported at home and abroad, and achieve the effect of efficient utilization and rich sources.

Inactive Publication Date: 2008-06-25
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Extracting natural antioxidant substances from emblica bark has not been reported at home and abroad so far

Method used

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  • Method of extracting natural oxidationresistant active substanceoil from orange tree bark
  • Method of extracting natural oxidationresistant active substanceoil from orange tree bark
  • Method of extracting natural oxidationresistant active substanceoil from orange tree bark

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method of extracting natural antioxidant active substances from the bark of Amla emblica is:

[0029] (1) pulverizing: the clean emblica bark raw material is pulverized with a plant pulverizer;

[0030] (2) Extraction: use water as solvent, and the solid-liquid mass volume ratio of crushed bark raw material and water is 1: 5~10g / ml, immerse the pulverized bark raw material in water, at 50~80 ℃ Under the action of temperature and ultrasonic waves, extract for 60-120 minutes to obtain the extract. Wherein the solid-liquid mass volume ratio can be any value in 1:5~10g / ml, such as 1:5g / ml, 1:7g / ml, 1:8g / ml, 1:10g / ml, etc., and the temperature can be For any value between 50 and 80°C, such as 50°C, 58°C, 64°C, 75°C, and 80°C, the extraction time can be 60min, 71min, 83min, 96min, 105min, 112min, 120min, etc.

[0031] (3) Flocculation and sedimentation: use chitosan acetic acid solution as flocculant, add chitosan acetic acid solution with a mass concentration of 0.5 to 1...

Embodiment 2

[0038] 1. Put 320g of emblica bark and 2500mL of extraction water after purification, air-drying and crushing (with a plant pulverizer, through a Φ2mm sieve plate) in a KH-200 DB numerically controlled ultrasonic generator tank, under the action of ultrasonic intensification to extract. The ultrasonic frequency is 20kHz, the ultrasonic power is 200W, the temperature is controlled at 65±5°C, and the extraction time is 60min. When the time comes, take it out, filter to remove the slag, and obtain 2180 mL of extract.

[0039] 2. Concentrate the extract to 1100mL, add 15mL of chitosan acetic acid solution with a concentration of 1%, and leave it still after stirring while hot. After cooling to room temperature, it was placed in a refrigerator at 7 °C for 48 h. Separate clear liquid 1050mL, measure its solid content and be 6.5%, total solid content is 71.5g.

[0040] 3. Add 12g of activated carbon and 0.4g of EDTA-2Na to the clear solution at 80°C, stir for 0.5h, filter to remov...

Embodiment 3

[0045] 1. Place 350g of emblica bark and 2450mL of extraction water pretreated according to the method of Example 1 in a KH-200 DB type numerically controlled ultrasonic generator tank, and extract under ultrasonic strengthening. The ultrasonic frequency is 40kHz, the ultrasonic power is 100W, the temperature is controlled at 70±5°C, and the extraction time is 90min. When the time comes, take it out, filter to remove the slag, and obtain 2100 mL of extract.

[0046] 2. Add 17 mL of chitosan acetic acid solution with a concentration of 1% to the extract, stir vigorously and let it stand to cool to room temperature, put it in a refrigerator at 5°C for 24 hours, and then separate out 2000 mL of the clear liquid.

[0047] 3. Add 14g of activated carbon and 0.4g of EDTA-2Na to the clear liquid at 85°C, stir for 1 hour, filter to remove carbon, and use the filtrate as the upper column liquid for resin adsorption separation.

[0048] 4. Add the clear liquid to the pretreated AB-8 ty...

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Abstract

The invention relates to a method of extracting natural anti-oxidation active substance from plant materials, which discloses a method of extracting natural anti-oxidation active substance from oil orange tree bark. The method includes the following steps: (1) crushing; (2) extracting: the water is the solvent, the mass of the bark materials which have been crushed to the volume of water is 1 : 5g / ml to1: 10g / ml, the bark materials crushed are immersed in the water, the extracting solution is gained under the temperature ranging from 50 DEG C to 80 DEG C and under the effect of ultrasonic; (3) flocculating settling: the chitosan acetic acid solution is the flocculating agent; and the chitosan acetic acid solution is added into the extracting solution and stirred for 10 minutes to 30 minutes; (4) subzero fractionating: the extracting solution which is added with the flocculating agent is cooled and kept statically, the clear solution is extracted and the sediment is threw away; (5) the clear solution is added with 15 percent to 20 percent active carbon and 0.4 percent to 0.6 percent EDTA-2Na which are fixedly contained in the clear solution, the clear solution is filtered for the cleaning of the carbon after the clear solution is stirred for 0.5 hour to 1 hour, the filtrate is used as the upper-column liquid for the fractionation by adsorption of the resin; (6) fractionation by adsorption of the resin, by using desolvation, the powdered natural anti-oxidation active substance is gained by the vacuum freeze drying.

Description

technical field [0001] The invention relates to a method for extracting natural antioxidant active substances from plant materials, in particular to a method for extracting natural antioxidant active substances from the bark of Amla emblica. Background technique [0002] Emblica (Phyllantus emblica) has a wide distribution of resources in southern my country. The fruit of Emlica emblica has medicinal functions such as clearing away heat, cooling blood, invigorating digestion, invigorating the stomach, promoting body fluid and relieving cough, and inhibiting bacteria. It can be processed into food, beverages, health products, medicines and cosmetics. In recent years, the development and utilization of emblica resources mainly focus on the artificial cultivation and fruit processing and utilization of emblica. [0003] The bark of Amla emblica is rich in plant polyphenols, and its main component is a mixture (3:1) of partially citrusylated (about 25%) polymeric prodelphinidin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/47A61P39/06A61K129/00
Inventor 陈笳鸿汪咏梅吴冬梅吴在嵩徐曼
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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