Nano contact printing
A surface contact, molecular technology, applied in nanotechnology, nanotechnology, nanotechnology for information processing, etc., can solve problems such as large amount of time, and achieve the effect of repeatability improvement
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[0098] Example 1: Preparation of complementary images of DNA monolayers
[0099] A. Preparation of DNA solution
[0100] Before use, use 75% H 2 SO 4 And 25% H 2 O 2 The solution cleans all glass instruments. All the water used is ultrapure water (18MΩ / cm).
[0101] The first DNA 5'- / 5-thiol MC6-D / ACG CAA CTT CGG GCT CTT-3' was purchased from Integrated DNA Technologies, Inc. (IDT), Coraville, IA. All DNA strands are used in the state accepted from the manufacturer. The first DNA was dissolved in water at a concentration of 1 μg / mL, divided into smaller aliquots of 50 μL, and stored at -20°C. When a portion of this solution is used, it is reduced by placing an aliquot in a 40 mM buffer solution (0.17 M sodium phosphate, pH 8) containing dithiothreitol (DTT) for 16 hours. According to the manufacturer's instructions, size exclusion chromatography (NAP 10 column from Pharmacia Biotech) was used to separate the oligonucleotides and the by-products of the DTT reaction. A 10 mM sodiu...
Example Embodiment
[0110] Example 2: Pattern transfer of gold grid
[0111] The AFM calibration gold grid was soaked in the 4 μM solution of the first DNA molecule described in Example 1 for 5 days to produce a patterned body. The main body was exposed to a 1 mM 6-mercapto-1-hexanol aqueous solution for 2 hr to minimize non-specific adsorption of single-stranded DNA, and then rinsed with water 5 times and air-dried. The subject was then exposed to the 6 μM solution of the second DNA described in Example 1 for 2 hours, so that hybridization occurred. Place the gold substrate on the second mica on the main body so that the two gold surfaces face each other with a small amount of water between them. A small mechanical force is applied to push the two substrates together. After about 5 hours, the substrate was soaked in 1M NaCl (70°C) in TE buffer for 20 minutes. The two substrates (ie, the main body and the complementary image) are automatically separated, rinsed twice with 1M NaCl in TE buffer, and r...
Example Embodiment
[0112] Example 3: DNA chip manufacturing
[0113] As described in Demer et al., Angew. Chem. Int. Ed. (2001), 40:307 1-3073, the main body was prepared using dip pen nanolithography, and the entire teaching of this document is incorporated herein by reference. To prepare the main body, the gold surface on the mica substrate was contacted with a 1 mM solution of 1-octadecanethiol (ODT) in ethanol for 5 minutes to cover the exposed gold surface with ODT molecules. The substrate was then immersed in a 1 mM solution of 1,16-mercaptohexadecanoic acid (MHA), and the tip of the atomic force microscope was used to make the displacement bound to the surface by contacting the surface with a force of about 0.5nN to produce a 100nm dot. ODT molecule. The MHA in the solution binds to the exposed gold surface of the dots. Activate MHA with a 10 mg / mL solution of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC) in 0.1M morpholine / ethanesulfonic acid at pH 4.5 The carboxylic ac...
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