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Cell-less composite type artificial skin and preparation thereof

A kind of artificial skin and composite technology, applied in coating, medical science, prosthesis, etc., can solve the problems of unfavorable wound epidermal cell crawling and wound sealing, difficult to observe wound infection, complicated production process, etc., to promote Effects of treatment and healing, prevention of recurrence, and promotion of healing

Active Publication Date: 2008-08-06
西安组织工程工程技术研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because it contains living cell components, it has the disadvantages of long production time, short storage time, complex production process, and high cost.
However, Biobrane and Integra, two-layer skin products that do not contain living cell components, have a non-degradable silicone film on the surface, which makes it difficult to observe the early infection of the wound, which is not conducive to the crawling of wound edge epidermal cells and the sealing of the wound. The silicone film will be removed later. Second surgery is also required

Method used

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  • Cell-less composite type artificial skin and preparation thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Step 1. The preparation of the dermal layer material refers to the Chinese patent (CNZL No.: 200510096463.3) "Preparation method of tissue engineered extracellular matrix" to obtain a loose and porous acellular dermal matrix material, which is a circle with a diameter of 3 cm and a thickness of 0.5 mm .

[0023] Step 2. Loading of growth factors: under sterile conditions, human recombinant EGF (rhEGF, Sigma company) was prepared into EGF aqueous solution, and 2 mg of freeze-dried gelatin microspheres (provided by the Biomacromolecular Materials Laboratory of Western Polytechnic University) were added to the EGF solution. , 4 ° C to fully soak the EGF solution into the freeze-dried gelatin microspheres.

[0024] Step 3. Preparation of epidermis material: select biological protein glue to be prepared into a gel solution, under aseptic conditions, add 2 mg of gelatin microspheres containing EGF to the gel solution of 10 ml and stir evenly, so that the final concentration o...

Embodiment 2

[0027] Step 1. The dermis layer material is prepared from a mixture of collagen, chitosan and chondroitin sulfate to form a recombined dermal matrix, which is a loose and porous extracellular matrix material with a diameter of 5 cm and a thickness of 5 mm.

[0028] Step 2, drug loading: prepare penicillin into penicillin aqueous solution under aseptic conditions, add 2 mg of freeze-dried medical protein colloid particles to the penicillin solution, and fully soak the penicillin solution into the freeze-dried particles at 4°C.

[0029]Step 3. Preparation of epidermis material: under sterile conditions, add penicillin-containing microparticles into 10 ml of polylactic acid gel solution and stir evenly so that the final concentration of penicillin is 800 i.u / ml.

[0030] Step 4. Construction of composite skin: the gel solution containing microparticles is pressed at 2 mg / cm 2 It is coated on the upper surface of the dermis material, placed in a vessel to solidify at 4°C, then pla...

Embodiment 3

[0032] Step 1. The dermis layer is made of loose and porous decellularized cowhide matrix material (available from Sigma), with a diameter of 7 cm and a thickness of 2 mm. Prepare the polyglycolic acid gel solution as the cuticle material.

[0033] Step 2. The construction of composite skin: the polyglycolic acid gel solution is pressed at 1mg / cm 2 Evenly coated on the upper surface of the dermis material, placed in a vessel to solidify at 4°C, then placed in a -80°C refrigerator for 16 hours, and then vacuum freeze-dried to obtain a cell-free composite artificial skin product. The preparation steps of the skin are simple, the storage time is long, and the skin can be widely used in the treatment of burn and scald wounds.

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Abstract

The invention relates to a cell-free compound artificial skin and a preparation method thereof. The compound skin of the invention is provided with a double layer structure of a dermis layer and an epidermis layer; the dermis layer is a loose porous structure made of extracellular matrix; gel-shaped epidermis-like materials are coated on the upper surface of the dermis layer to solidify, thus forming the epidermis layer, and the epidermis layer is inserted into a void structure of the surface of the dermis layer; compared with the existing skin substitutes, the skin of the invention has the functions of promoting the regeneration of wound skin, enhancing the elasticity, flexibility and mechanical wear resistance of the skin after the wound is healed, reducing the scar proliferation, controlling the contracture, improving the success rate of skin implantation and improving the healing quality; the skin of the invention has a sustained release function and can make individual-based treatment for different diseases by releasing different drugs and being directly applied to the treatment of skin defects caused by inflammations, ulcers, burn wounds and iatrogenic causes; the epidermis layer and the dermis layer are embedded together organically with a tight structure; the shapes, sizes and the thicknesses of the skin can be prepared according to specific requirements. The skin of the invention has wide sources of preparation materials, simple production process, short production period, long period of product storage and convenient transportation.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering of biological materials, and in particular relates to a cell-free composite skin and a preparation method thereof. Background technique [0002] The skin is the largest organ of the human body, and is a natural barrier and a bridge of communication between the body and the outside world. Skin defects can affect the patient's appearance in mild cases, and in severe cases can cause infection or a large loss of electrolytes and water, leading to the death of the patient. The key to treating large-area skin defects is to seal the wound as soon as possible, reduce the consumption of the body and the disorder of the internal environment, and prevent the invasion of harmful substances. [0003] With the development of tissue engineering technology, tissue-engineered skin has become a hot spot in the treatment of skin defects, but so far no ideal skin substitute has been applied. Ideal tissue...

Claims

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Application Information

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IPC IPC(8): A61L27/44A61L27/34A61L27/20A61L27/24A61L27/60A61L27/54A61L27/56
Inventor 金岩陆伟张勇杰
Owner 西安组织工程工程技术研究中心
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