Composite automatic induction culture medium for expressing exogenous protein by pronucleus expression system

A technology for inducing medium and exogenous protein, applied in the field of compound automatic induction medium, can solve the problems of hindering the expression of exogenous protein and affecting the bacterial density, and achieve the goals of saving medium cost, increasing protein production, and facilitating practical operation Effect
CN101235362AInactive Publication Date: 2008-08-06HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
0 Cites 19 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Publication Date
2008-08-06
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a complex autoinduction culture medium which utilizes a prokaryotic expression system to express heterologous protein, each liter culture medium comprises the following components in weight such as tryptone 10-40g, yeast extract 5-20g, six hydrate sodium succinate 0-8.1g, two hydrate sodium citrate 0-1.5g, glycerin 15-50g, glucose 0.5g, milk sugar 2g, monobasic sodium phosphate 3.55g, monopotassium phosphate 3.40g, ammonium chloride 2.68g, sodium sulfate 0.71g, magnesium sulfate heptahydrate 0.50g and iron chloride hexahydrate 0.03g. Compared with normal culture medium LB, the yield of expression level of heterologous protein improves more than 8 times through utilizing complex autoinduction culture medium of the invention to express heterologous protein, compared with the same type complex autoinduction culture medium, the yield improves more than 2 times.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a culture medium, in particular to a compound auto-induction culture medium for expressing foreign proteins in a prokaryotic expression system controlled by a lac promoter and a T7lac promoter, and belongs to the field of biotechnology. Background technique

[0002] The maturity of DNA sequencing technology can accurately provide the coding sequences of tens of thousands of proteins from different species. These coding sequences can be cloned into expression vectors by using DNA recombination technology to realize the expression of the target protein. In recent years, the use of genetic engineering to produce commercial proteins has increased dramatically, and these expressed recombinant proteins have been widely used in industry and medicine. Escherichia coli has the advantages of clear genetic background, easy operation, rapid growth, high expression level, and low culture cost, plus years of experience in exogenous gene exp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More