Liquid composite microbial fertilizer and its preparation method

A technology of compound microorganisms and microorganisms, applied in the direction of organic fertilizers, etc., can solve the problems of complex production process, long production cycle, large equipment investment, etc., and achieve the effect of improving soil structure, improving crop quality, and improving soil water and fertilizer retention performance

Inactive Publication Date: 2008-08-13
INST OF SOIL & FERTILIZER SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But its disadvantage is that using the above three types of fertilizers alone can only solve one aspect of the nutrition required by plants.
已有的中国专利文件CN1134408A(95104927.5),CN1191854A(98100645.0),CN1587218A(200410052666.8),CN1600751A(200410050683.8),CN1654435A(200410065948.1),CN1911870A(200610068499.5)都是采用不同的微生物菌种,通过分别培养、发酵后 , the products are combined in proportion to form a compound microbial fertilizer. The main problem of using the existing process to produce microbial fertilizer is that the equipment for the separate fermentation of each strain has a large investment and a long production cycle, resulting in high production costs and complicated production processes.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0017] A preparation method of liquid compound microbial fertilizer, the steps are as follows:

[0018] 1. Preparation of Mixed Fermentation Broth

[0019] (1) Four kinds of microbial strains of Bacillus subtilis, Bacillus megaterium, silicate bacteria and Streptomyces jingyang were cultured in shake flasks respectively to obtain Bacillus subtilis bacteria liquid, Bacillus megaterium bacteria liquid, and silicate bacteria bacteria liquid Streptomyces and Streptomyces jingyang bacteria liquid four bacterial liquids,

[0020] (2) The Bacillus subtilis bacterium liquid and the Bacillus megaterium bacterium liquid of step (1) are respectively entered into the seed tank for mixed fermentation culture according to the inoculum amount of 3%, to obtain the fermentation bacterium liquid I;

[0021] (3) The silicate bacteria bacterium liquid of step (1) and the Streptomyces jingyang bacterium liquid are respectively entered into the seed tank by 3% inoculum amount and then mixed and fe...

Embodiment 1

[0070] Embodiment 1: the preparation of the mixed fermented liquid of four kinds of microorganisms

[0071] (1) Cultivation of Bacillus subtilis liquid

[0072] Medium: Potato (peeled, boiled) 200 grams

[0073] Sucrose (white sugar or glucose) 20 grams

[0074] 1000ml distilled water

[0075] pH6.0~6.5

[0076] 15-20 grams of agar

[0077] Inject 150ml of culture medium into a 500ml Erlenmeyer flask; use 5ml of sterile water to transfer the strains on the slant to corresponding shake flasks, and cultivate them at 28-30°C and 200 rpm for 24 hours.

[0078] (2) Cultivation of Bacillus megaterium liquid

[0079] Medium: beef extract 5.0g

[0080] Peptone 5.0 g

[0081] Sodium chloride 5.0 g

[0082] pH 7.2~7.4

[0083] 1000ml distilled water

[0084] 15-18 grams of agar

[0085] Inject 150ml of culture medium into a 500ml Erlenmeyer flask; use 5ml of sterile water to transfer the strains on the sl...

Embodiment 2

[0155] Embodiment 2: Liquid compound microbial fertilizer

[0156] 10 kilograms of fermented liquid of four kinds of microorganisms prepared in embodiment 1, 90 kilograms of clean water, 12.28 kilograms of soluble nitrogen, phosphorus and potassium fertilizers that crossed 80 mesh sieves after pulverizing (wherein, 6.76 kilograms of ammonium sulfate, 1.32 kilograms of urea, 2.2 kilograms of diammonium phosphate, potassium sulfate 2.0 kg).

[0157] Preparation method: add clean water to the fermented liquid of four microorganisms and mix evenly, dissolve soluble nitrogen, phosphorus and potassium fertilizer in the aqueous solution of the fermented liquid of four microorganisms, mix evenly, and then pack, test and leave the factory.

[0158] The fertilizer is suitable for field crops, facility vegetables and open field vegetables.

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Abstract

A liquid composite microorganism fertilizer and the preparation method thereof belong to the field of the microorganism fertilizer technique. The invention uses four microorganisms of bacillus subtilis, bacillus magaterium, silicate bacterium and Streptomyces Jingyingensis for mixed-zymolysis and is combined with soluble nitrogen, phosphorus and potassium to prepare the liquid composite microorganism fertilizer. The fertilizer can be used for all type of soil and crops, the application effect on vegetable, fruit is optimum, and the fertilizer has the effects of improving the soil structure, increasing the water-keeping and fertilizer-keeping capability of the soil, and better increasing the production and improving the crop quality.

Description

technical field [0001] The invention relates to a liquid compound microbial fertilizer and a preparation method thereof, belonging to the technical field of microbial fertilizers. Background technique [0002] In 1891, Beijerinck completed the pure culture of rhizobia and nitrogen-fixing bacteria for the first time, and began the production and application of microbial fertilizers. In 1895, the commercial rhizobia inoculant was launched on the market (trade name Nitrogen). From the 1930s to the 1940s, the United States, Australia, and the United Kingdom all had inoculum manufacturers. In 1930, the sales of rhizobia inoculants in the United States reached 1 million US dollars. After the 1950s, more than 70 countries in the world, including China, began to research, produce and use rhizobia inoculants, and the area continued to expand. In the 1970s, a series of research work on beneficial microorganisms such as nitrogen-fixing bacteria, phosphorus-solubilizing bacteria and po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08
Inventor 刘兆辉江丽华王梅林海涛贾翠香郑福丽宋效宗谭德水高新昊
Owner INST OF SOIL & FERTILIZER SHANDONG ACAD OF AGRI SCI
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