Liquid composite microbial fertilizer and its preparation method
A technology of compound microorganisms and microorganisms, applied in the direction of organic fertilizers, etc., can solve the problems of complex production process, long production cycle, large equipment investment, etc., and achieve the effect of improving soil structure, improving crop quality, and improving soil water and fertilizer retention performance
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[0017] A preparation method of liquid compound microbial fertilizer, the steps are as follows:
[0018] 1. Preparation of Mixed Fermentation Broth
[0019] (1) Four kinds of microbial strains of Bacillus subtilis, Bacillus megaterium, silicate bacteria and Streptomyces jingyang were cultured in shake flasks respectively to obtain Bacillus subtilis bacteria liquid, Bacillus megaterium bacteria liquid, and silicate bacteria bacteria liquid Streptomyces and Streptomyces jingyang bacteria liquid four bacterial liquids,
[0020] (2) The Bacillus subtilis bacterium liquid and the Bacillus megaterium bacterium liquid of step (1) are respectively entered into the seed tank for mixed fermentation culture according to the inoculum amount of 3%, to obtain the fermentation bacterium liquid I;
[0021] (3) The silicate bacteria bacterium liquid of step (1) and the Streptomyces jingyang bacterium liquid are respectively entered into the seed tank by 3% inoculum amount and then mixed and fe...
Embodiment 1
[0070] Embodiment 1: the preparation of the mixed fermented liquid of four kinds of microorganisms
[0071] (1) Cultivation of Bacillus subtilis liquid
[0072] Medium: Potato (peeled, boiled) 200 grams
[0073] Sucrose (white sugar or glucose) 20 grams
[0074] 1000ml distilled water
[0075] pH6.0~6.5
[0076] 15-20 grams of agar
[0077] Inject 150ml of culture medium into a 500ml Erlenmeyer flask; use 5ml of sterile water to transfer the strains on the slant to corresponding shake flasks, and cultivate them at 28-30°C and 200 rpm for 24 hours.
[0078] (2) Cultivation of Bacillus megaterium liquid
[0079] Medium: beef extract 5.0g
[0080] Peptone 5.0 g
[0082] pH 7.2~7.4
[0083] 1000ml distilled water
[0084] 15-18 grams of agar
[0085] Inject 150ml of culture medium into a 500ml Erlenmeyer flask; use 5ml of sterile water to transfer the strains on the sl...
Embodiment 2
[0155] Embodiment 2: Liquid compound microbial fertilizer
[0156] 10 kilograms of fermented liquid of four kinds of microorganisms prepared in embodiment 1, 90 kilograms of clean water, 12.28 kilograms of soluble nitrogen, phosphorus and potassium fertilizers that crossed 80 mesh sieves after pulverizing (wherein, 6.76 kilograms of ammonium sulfate, 1.32 kilograms of urea, 2.2 kilograms of diammonium phosphate, potassium sulfate 2.0 kg).
[0157] Preparation method: add clean water to the fermented liquid of four microorganisms and mix evenly, dissolve soluble nitrogen, phosphorus and potassium fertilizer in the aqueous solution of the fermented liquid of four microorganisms, mix evenly, and then pack, test and leave the factory.
[0158] The fertilizer is suitable for field crops, facility vegetables and open field vegetables.
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