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Method for producing alcohol by starchy materials big tank and non-vector immobilized yeast continuous fermentation

A carrier-free immobilization technology of starchy raw materials, applied in the field of microbial fermentation engineering, can solve the problems of reduced equipment utilization, low resource utilization, and increased sewage load, so as to reduce the formation of retained materials, improve the growth environment, The effect of improving the utilization rate of starch

Active Publication Date: 2011-06-22
LINYI JINYIMENG BIOTECH
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AI Technical Summary

Problems solved by technology

[0004] With the continuous development of fermentation technology, continuous fermentation has gradually been recognized and accepted by people, and has been continuously applied to large-scale production in factories. And other factors also make many advantages of continuous fermentation production can not be reflected, and seriously also restrict the development of production
In particular, the influence of factors such as bacteria contamination in fermented seed tanks and high unit consumption of yeast makes the conversion rate of alcohol in continuous fermentation production low and the cost of alcohol production high. Therefore, the current fermentation production process is still in the mode of intermittent, semi-continuous and continuous. Constantly transform and replace
This not only increases the sewage load, but also reduces the utilization rate of equipment and the status quo of low starch conversion rate.
The pollution of the real environment, low resource utilization and high energy consumption will greatly restrict the survival and development of enterprises

Method used

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  • Method for producing alcohol by starchy materials big tank and non-vector immobilized yeast continuous fermentation
  • Method for producing alcohol by starchy materials big tank and non-vector immobilized yeast continuous fermentation

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Experimental program
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Embodiment

[0043] Embodiment: Large tank, the method for continuous fermentation of immobilized yeast without carrier to produce alcohol

[0044] 1. Pretreatment of starchy raw materials

[0045]The starchy raw material is pulverized in one stage, and the particle size is controlled at 1.5-1.8 mm, then stirred with water at 55-65 °C, and the pulverized raw material is mixed with distilled waste water and fresh water (weight ratio 60:40) to obtain For powder slurry, add high-temperature-resistant α-amylase to the prepared powder slurry, and add it continuously and uniformly in an amount of 2 to 5 μ / g raw materials. After stirring evenly, seal and heat to 67-89°C in the slurry tank, and maintain it for 30 ~60 minutes.

[0046] Then the powder slurry is cooked at medium temperature, the cooking temperature is controlled at 100-110 °C, the cooking temperature is 2 hours, the gelatinization rate is controlled at 85-95%, the paste liquid is vacuum-cooled to 60-62 °C, glucoamylase is added, mi...

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Abstract

The invention relates to a method for using starchiness as raw materials to produce alcohol through continuously fermenting with immobilized yeast without a carrier in a big tank, which comprises following steps: pre-processing the raw material of the starchiness, continuously fermenting and distilling with the immobilized yeast without the carrier, wherein the single tank volume of fermentation tanks is increased in continuous fermentation with immobilized yeast without the carrier, reducing the number of the fermentation tanks, shortening the connection length of fermentation, simultaneously, adopting slope hearth, reducing the formation of retention materials, prearranging a fermentation tank, and adopting a multi-tank feed adding method feed sugar solution to the prearranged fermentation tank and a fermentation seed tank. The method of the invention provides a technique with high safety and high efficiency for producing the alcohol through continuously fermenting, simultaneously, the availability ratio of starch is also increased, the fermentation time is shortened, the wastewater emission is reduced, the load of wastewater treatment is alleviated, and the production cost is lowered.

Description

technical field [0001] The invention relates to a large tank of starch raw material and a method for continuous fermentation of immobilized yeast without carrier to produce alcohol, belonging to the technical field of microbial fermentation engineering. Background technique [0002] Alcoholic fermentation is a process in which yeast converts fermentable sugars into alcohol and carbon dioxide through the action of intracellular alcoholic enzymes. It is a complex biochemical process. [0003] The traditional alcoholic fermentation in our country is to cultivate distiller's mother in the laboratory. The distiller's mother is expanded and cultivated in multiple stages, and finally to Dajiufu, and then used as a fermented seed. In terms of fermentation, batch fermentation is mainly used, and the volume of the fermenter is small. [0004] With the continuous development of fermentation technology, continuous fermentation has gradually been recognized and accepted by people, and h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/06C12R1/645
CPCY02E50/17Y02E50/10
Inventor 张超张立省郑启
Owner LINYI JINYIMENG BIOTECH
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