D-lactic acid-producing strain with high optical purity and process for producing D-lactic acid by fermentation thereof

A technology of optical purity and production of bacteria, applied in fermentation, microbial-based methods, bacteria, etc., can solve the problems of low yield, difficult operation and control, etc., and achieve simple formula, low production cost, great social significance and economic value Effect

Inactive Publication Date: 2008-10-15
NANJING UNIV OF TECH
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Problems solved by technology

In 2004, Ding Zijian of our laboratory reported for the first time the process of using Sporolactobacillus sp. to ferment D-lactic acid from glucose. After 72 hours of fermentation, the yield of D-lactic acid was 40.7g/L, and the optical purity reached 96.04%. The optical purity of the product of the process is high, but the yield is relatively low; in 2006, Yang Wenge et al. reported the process of producing D-lactic acid by using Lactobacillus

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  • D-lactic acid-producing strain with high optical purity and process for producing D-lactic acid by fermentation thereof
  • D-lactic acid-producing strain with high optical purity and process for producing D-lactic acid by fermentation thereof

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Example Embodiment

[0034] Example 1

[0035] This example illustrates the breeding method of Bacillus sp. Y2-8.

[0036] 1. Screen the original strain producing D-lactic acid from soil:

[0037] The high-sugar-tolerant microorganisms in the soil samples in Nanjing area were enriched and cultured with 100g / L glucose high-sugar liquid medium, and then the high-sugar-tolerant microorganisms were subjected to 100g / L glucose-bromocresol green color development plate under anaerobic culture conditions. The acid-producing strains were isolated, and the strains with large diameter of discoloration circle and fast discoloration speed were selected for acid-producing fermentation research. The optical purity of the product was detected by high performance liquid chromatography with a chiral stationary phase method, the ligand exchange column model was Sumichiral CA5000, and the mobile phase was 1mM CuSO 4 Solution, UV detection wavelength is 254nm. A D-lactic acid strain was obtained and named as Sporo...

Example Embodiment

[0048] Example 2

[0049] This example illustrates the process of producing D-lactic acid by fermentation of Bacillus sp. Y2-8.

[0050] Plate medium: glucose 10%, yeast extract 0.2%, anhydrous sodium acetate 0.2%, anhydrous magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.2%, agar 1.8%, water balance, pH 7.0;

[0051] Seed medium: glucose 2%, yeast extract 0.3%, corn steep liquor 0.4%, bran 0.3%, anhydrous magnesium sulfate 0.02%, ferrous sulfate 0.001%, manganese sulfate 0.001%, calcium carbonate 1.5%, water balance, pH7.0;

[0052] Fermentation medium: glucose 10%, corn steep liquor 1.5%, anhydrous magnesium sulfate 0.05%, ferrous sulfate 0.001%, manganese sulfate 0.001%, water balance, pH 7.0.

[0053] Bacillus sp. Y2-8 was inoculated into a plate medium and cultured in an anaerobic incubator at a culture temperature of 30°C and a culture time of 36h. Inoculate the cultured Bacillus sp. into a seed medium with a liquid volume of 60 mL, add 10 glass beads, and c...

Example Embodiment

[0055] Example 3

[0056] This example illustrates the process of producing D-lactic acid by fermentation of Bacillus sp. Y2-8.

[0057] Plate medium: glucose 12%, yeast extract 0.1%, anhydrous sodium acetate 0.3%, anhydrous magnesium sulfate 0.03%, potassium dihydrogen phosphate 0.3%, agar 1.8%, water balance, pH 7.0;

[0058] Seed medium: glucose 5%, beef extract 0.6%, anhydrous magnesium sulfate 0.01%, ferrous sulfate 0.003%, manganese sulfate 0.005%, calcium carbonate 2%, water balance, pH 7.0.

[0059] Fermentation medium: corn saccharification liquid (glucose) 10%, yeast extract 0.2%, anhydrous magnesium sulfate 0.05%, ferrous sulfate 0.001%, manganese sulfate 0.001%, water balance, pH 7.0.

[0060] Bacillus sp. Y2-8 was inoculated into a plate medium and cultured in an anaerobic incubator at a culture temperature of 37°C and a culture time of 30h. Inoculate the cultured Bacillus sp. into the seed medium with a liquid volume of 60 mL, seal it with 2-3 cm thick liquid p...

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Abstract

The invention discloses a sporolactobacillus s p.Y2-8 strain which is a high-ptical purity D-lactic acid producing strain and a process for producing the D-lactic acid by the same through fermentation. The fermentation process comprises plate culture, seed culture and fermentation and acid production, wherein the plate culture, the seed culture and the fermentation and acid production are all performed under anaerobic conditions. The process is simple and requires no strict stepwise regulation. The fermentation process has the advantages of easily accessible culture medium, simple formula and low production costs. The purity of D-lactic acid produced by the strain and the fermentation process of the invention is up to 99.1 percent, with the D-lactic acid content in the fermentation liquid between 9.1 percent and 16.5 percent, and has significant social and economic value.

Description

technical field [0001] The invention relates to a high optical purity D-lactic acid producing bacterium Sporolactobacillus sp. Y2-8 strain and a process for fermenting and producing D-lactic acid, belonging to the field of organic acid fermentation production. Background technique [0002] Lactic acid, also known as α-Hydroxypropionic acid (α-Hydroxypropionic acid), is one of the three major organic acids recognized in the world. According to its optical properties, it can be divided into L-lactic acid, D-lactic acid and D, L- lactic acid. [0003] With the development of a series of new uses of D-lactic acid, the research on the application and production of D-lactic acid has attracted more and more attention. At present, the use of D-lactic acid is mainly manifested in: [0004] (1) D-lactic acid is the raw material for the synthesis of an excellent herbicide for the control of gramineous weeds - Puma Super and a new broad-spectrum high-efficiency herbicide - Weiba. Raw...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/56C12R1/01
Inventor 何冰芳柏中中许婷婷王丽娟欧阳平凯
Owner NANJING UNIV OF TECH
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