Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

D-lactic acid-producing strain with high optical purity and process for producing D-lactic acid by fermentation thereof

A technology of optical purity and production of bacteria, applied in fermentation, microbial-based methods, bacteria, etc., can solve the problems of low yield, difficult operation and control, etc., and achieve simple formula, low production cost, great social significance and economic value Effect

Inactive Publication Date: 2008-10-15
NANJING UNIV OF TECH
View PDF0 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2004, Ding Zijian of our laboratory reported for the first time the process of using Sporolactobacillus sp. to ferment D-lactic acid from glucose. After 72 hours of fermentation, the yield of D-lactic acid was 40.7g / L, and the optical purity reached 96.04%. The optical purity of the product of the process is high, but the yield is relatively low; in 2006, Yang Wenge et al. reported the process of producing D-lactic acid by using Lactobacillus combined fermentation technology. Starting from glucose, fermentation for 25 to 38 hours, the acid production can reach 75~131g / L lactic acid, the optical purity reaches 99%, but the process uses a combined fermentation method, that is, the fermentation process needs to be regulated in stages according to the three steps of aerobic fermentation, microaerobic fermentation and final anaerobic acidogenic fermentation. relatively difficult to control

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • D-lactic acid-producing strain with high optical purity and process for producing D-lactic acid by fermentation thereof
  • D-lactic acid-producing strain with high optical purity and process for producing D-lactic acid by fermentation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] This example illustrates the breeding method of Lactobacillus sporogenes Y2-8.

[0036] 1. Screen the original strains producing D-lactic acid from the soil:

[0037] 100g / L glucose high-sugar liquid medium was used to enrich and cultivate the high-sugar-tolerant microorganisms in soil samples in Nanjing area, and then the high-sugar-tolerant microorganisms were tested under anaerobic culture conditions by 100g / L glucose-bromocresol green color development plate. The acid-producing strains were isolated, and the strains with large diameter and fast discoloration were selected for acid-producing fermentation research. The optical purity of the product is detected by high performance liquid chromatography chiral stationary phase method, the ligand exchange column model is Sumichiral CA5000, and the mobile phase is 1mM CuSO 4 solution, the UV detection wavelength is 254nm. Obtain a strain of D-lactic acid bacteria, named Sporolactobacillus inulinus DX12, cryopreserved fo...

Embodiment 2

[0049] This example illustrates the process of producing D-lactic acid by fermentation of Lactobacillus sporogenes Y2-8.

[0050] Plate medium: glucose 10%, yeast extract 0.2%, anhydrous sodium acetate 0.2%, anhydrous magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.2%, agar 1.8%, water balance, pH7.0;

[0051] Seed medium: glucose 2%, yeast extract 0.3%, corn steep liquor 0.4%, bran 0.3%, anhydrous magnesium sulfate 0.02%, ferrous sulfate 0.001%, manganese sulfate 0.001%, calcium carbonate 1.5%, water balance, pH7.0;

[0052] Fermentation medium: glucose 10%, corn steep liquor 1.5%, anhydrous magnesium sulfate 0.05%, ferrous sulfate 0.001%, manganese sulfate 0.001%, water balance, pH 7.0.

[0053] Lactobacillus sporogenes Y2-8 was inoculated into the plate culture medium, placed in an anaerobic incubator for cultivation, the cultivation temperature was 30°C, and the cultivation time was 36h. Inoculate the Lactobacillus bacillus cultured on the plate into the seed m...

Embodiment 3

[0056] This example illustrates the process of producing D-lactic acid by fermentation of Lactobacillus sporogenes Y2-8.

[0057] Plate medium: glucose 12%, yeast extract 0.1%, anhydrous sodium acetate 0.3%, anhydrous magnesium sulfate 0.03%, potassium dihydrogen phosphate 0.3%, agar 1.8%, water balance, pH7.0;

[0058] Seed medium: glucose 5%, beef extract 0.6%, anhydrous magnesium sulfate 0.01%, ferrous sulfate 0.003%, manganese sulfate 0.005%, calcium carbonate 2%, water balance, pH 7.0.

[0059] Fermentation medium: corn saccharification solution (glucose meter) 10%, yeast extract 0.2%, anhydrous magnesium sulfate 0.05%, ferrous sulfate 0.001%, manganese sulfate 0.001%, water balance, pH 7.0.

[0060] Lactobacillus sporogenes Y2-8 was inoculated into the plate culture medium, placed in an anaerobic incubator for cultivation, the cultivation temperature was 37°C, and the cultivation time was 30h. Inoculate the Lactobacillus bacillus cultured on the plate into the seed medi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a sporolactobacillus s p.Y2-8 strain which is a high-ptical purity D-lactic acid producing strain and a process for producing the D-lactic acid by the same through fermentation. The fermentation process comprises plate culture, seed culture and fermentation and acid production, wherein the plate culture, the seed culture and the fermentation and acid production are all performed under anaerobic conditions. The process is simple and requires no strict stepwise regulation. The fermentation process has the advantages of easily accessible culture medium, simple formula and low production costs. The purity of D-lactic acid produced by the strain and the fermentation process of the invention is up to 99.1 percent, with the D-lactic acid content in the fermentation liquid between 9.1 percent and 16.5 percent, and has significant social and economic value.

Description

technical field [0001] The invention relates to a high optical purity D-lactic acid producing bacterium Sporolactobacillus sp. Y2-8 strain and a process for fermenting and producing D-lactic acid, belonging to the field of organic acid fermentation production. Background technique [0002] Lactic acid, also known as α-Hydroxypropionic acid (α-Hydroxypropionic acid), is one of the three major organic acids recognized in the world. According to its optical properties, it can be divided into L-lactic acid, D-lactic acid and D, L- lactic acid. [0003] With the development of a series of new uses of D-lactic acid, the research on the application and production of D-lactic acid has attracted more and more attention. At present, the use of D-lactic acid is mainly manifested in: [0004] (1) D-lactic acid is the raw material for the synthesis of an excellent herbicide for the control of gramineous weeds - Puma Super and a new broad-spectrum high-efficiency herbicide - Weiba. Raw...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12P7/56C12R1/01
Inventor 何冰芳柏中中许婷婷王丽娟欧阳平凯
Owner NANJING UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com