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Microbial immobilization method

A technology for microorganisms and vegetable oils, applied in directions such as being fixed on/in an organic carrier, can solve the problems of difficulty in industrialization, inability to prepare calcium alginate gel beads, and small preparation scale, and achieve good microbial cell activity. , Not easy to leak, easy to operate

Inactive Publication Date: 2008-12-10
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

The existing alginate immobilized cells mainly use spray drying method, orifice extrusion method, titration method, electrostatic titration method to prepare gel beads, but these methods have many problems, such as: cannot prepare algae with a particle size of less than 200 microns Calcium acid gel beads, and the preparation scale is small, it is difficult to realize industrialization (Liu Xiudong, He Yang, Ma Xiaojun, etc. Microcapsules and their applications in the field of biomedicine. Science Bulletin, 2000, 45(23): 2476-2485 )

Method used

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Embodiment 1

[0038] 1) At room temperature, a sodium alginate solution uniformly mixed with brewer's yeast and nano-scale calcium carbonate is added to the dispersion medium as a dispersed phase, and then stirred and emulsified at a stirrer speed of 150 rpm for 5 minutes to form an emulsion;

[0039] The weight-volume concentration of sodium alginate solution in the dispersed phase is 1.5%, and the weight-volume concentration of nanoscale calcium carbonate in the dispersed phase is 0.020%; the dispersed medium is liquid paraffin containing span85, and the volume ratio of the dispersed phase and the dispersed medium is 1:3;

[0040] The concentration of microbial seeds in the dispersed phase is 10 5 cfu / ml;

[0041] The volume percentage of span85 in the dispersion medium is 0.1%;

[0042] 2) Add liquid paraffin containing 5% glacial acetic acid to the above emulsion to adjust the pH of the emulsion to 6, and continue to stir for 5-10 minutes to form calcium alginate gel beads embedded wi...

Embodiment 2

[0053]Except that the volume ratio of the dispersed phase to the dispersion medium is 2:3, other conditions are the same as in the specific example 1.

[0054] Under this condition, the average particle diameter of the microcapsules is 372 μm (see accompanying drawing 1, table 1).

Embodiment 3

[0056] 1) At room temperature, a sodium alginate solution uniformly mixed with lactic acid bacteria and nano-scale calcium carbonate is added to the dispersion medium as a dispersed phase, and then stirred and emulsified at a stirrer speed of 1000 rpm for 10 minutes to form an emulsion;

[0057] The weight-volume concentration of the sodium alginate solution in the dispersed phase is 2.0%, and the weight-volume concentration of nanoscale calcium carbonate in the dispersed phase is 0.010%; the dispersed medium is vegetable oil containing span85, and the volume ratio of the dispersed phase to the dispersed medium is 1 : 5;

[0058] The concentration of microbial seeds in the dispersed phase is 10 6 cfu / ml;

[0059] The volume percentage of span85 in the dispersion medium is 2%;

[0060] 2) Add vegetable oil containing 10% glacial acetic acid in the above emulsion to adjust the pH of the emulsion to 5, and continue to stir for 10 minutes to form calcium alginate gel beads embed...

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Abstract

The invention relates to a microorganism immobilization method. The method comprising the following steps of: taking a sodium alginate solution containing dense suspending microorganisms and nano calcium carbonate as disperse phases, taking atoleine or vegetable oil as a disperse medium, and taking Span85 as a surfactant; forming droplets through agitation and emulsification; then preparing calcium alginate bead gel embedding the microorganisms through a gel initiator of glacial acetic acid; forming a polycation-polysnion complexing membrane through the reaction and cross linking of the bead gel and a chitosan solution; and finally forming a sodium alginate-chitosan microcapsule embedding the microorganisms. The method has mild conditions in the preparation process, simple requirements on apparatuses and equipment, easy mass production, good maintenance of the activity of the microorganism cells in the preparation process, and lower cell leakage rate. Thus, the technology is suitable for the immobilization embedding of a plurality of microorganisms such as yeast, bacillus coli, lactic acid bacteria, nitrobacteria, etc., thereby realizing high-density and large-scale microorganism culture.

Description

technical field [0001] The invention relates to the immobilization of microorganisms, in particular to a method for preparing alginic acid-chitosan microcapsules immobilized by an emulsification-internal gelation system. Background technique [0002] Immobilized microorganism technology has very good advantages, such as high density of microorganisms in a certain space area, the problem of microbial loss is eliminated, the reaction speed is accelerated, and the post-fermentation process is simple and convenient. That is: immobilized microorganism technology constitutes an efficient, rapid and continuous processing fermentation system, which is widely used in many fields. Among the many immobilization methods, the alginate gel embedding method is widely used, and its application is relatively mature. This method has the advantages of mild conditions, good cell stability, a large number of carrying cells, and is not easy to leak (Xiao Meiyan, Xu Erni, Chen Zhiwen. Research pr...

Claims

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Application Information

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IPC IPC(8): C12N11/04
Inventor 马小军林军章于炜婷孙志杰吕国军
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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