Unlock instant, AI-driven research and patent intelligence for your innovation.

Diazepam colloidal gold method detection test paper and preparation method thereof

A technology for detecting test paper and colloidal gold, which is applied in the field of determination of biological immunological methods, can solve problems such as not being able to adapt to high-throughput rapid detection, and achieve the effect of being economical, fast, easy to use, and low in cost

Inactive Publication Date: 2012-09-05
JIANGNAN UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection of diazepam at home and abroad mainly includes high performance liquid chromatography (HPLC), gas chromatography (GC), thin layer chromatography (TLC), gas-mass spectrometry (GC-MS), liquid-mass However, these methods not only require expensive instruments and equipment, but also have relatively high requirements for inspection materials, which require further purification before they can be carried out, and cannot be adapted to high-throughput, on-site rapid detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Diazepam colloidal gold method detection test paper and preparation method thereof
  • Diazepam colloidal gold method detection test paper and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: the preparation of diazepam detection test paper

[0017] (1) Diazepam is coupled with bovine serum albumin molecules as a diazepam-coupled antigen,

[0018] (2) Anti-diazepam polyclonal antibody obtained by immunization with diazepam-conjugated antigen:

[0019] (3) chloroauric acid is reduced to make 20nm-40nm colloidal gold particles with trisodium citrate reducing agent;

[0020] (4) Adjust 3 mL of colloidal gold to pH 9, add 0.05 mL of anti-diazepam polyclonal antibody with a protein concentration of 0.2 mg / mL drop by drop under stirring, add 5% bovine serum albumin (BSA) solution after standing for 30 min to make the final The concentration is 1%, after standing for at least 30min, centrifuge at 10000 rpm for 50min, remove the supernatant and resuspend with 3mL of 0.002mol / L pH9.0 borate buffer (containing 5% sucrose), repeat twice, and finally Dissolve with 0.3mL buffer to obtain stable anti-diazepam polyclonal antibody-colloidal gold marker;

[...

Embodiment 2

[0023] Example 2: Test paper requirements

[0024] (1) Conformity rate of negative reference product

[0025] Use phosphate buffered saline (PBS: 0.01mol / L, pH 7.4) to prepare a standard solution of promethazine hydrochloride with a concentration of 10ng / mL for 10 parallel detections, observe the results with normal or corrected eyesight, and observe the reaction time at 5 minutes The result should be negative.

[0026] Use phosphate buffered saline (PBS: 0.01mol / L, pH 7.4) to prepare a barbiturate standard solution with a concentration of 10ng / mL for 10 parallel detections, observe the results with normal or corrected vision, and observe the results when the reaction time is 5 minutes , should be negative.

[0027] (2) Conformity rate of positive reference products

[0028] Diazepam standard substances with concentrations of 50, 100, and 150 ng / mL were prepared in phosphate buffered saline (PBS: 0.01mol / L, pH 7.4) for parallel detection, and 10 parallel experiments were ca...

Embodiment 3

[0035] Embodiment 3: Method of using test paper

[0036] (1) Sample preparation

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a diazepam colloidal gold detection test paper and a preparation method thereof and belongs to the technical field of the detection of biological immunization. The detection test paper provided by the utility model consists of a sample gasket, a bonding and releasing gasket, nitrocellulose membrane, an absorbent gasket and a plastic bottom board; and the sample gasket, the bonding and releasing gasket, the nitrocellulose membrane and the absorbent gasket are pasted on the plastic bottom board in sequence. The bounding and releasing gasket is coated with an anti-diazepam polyclonal antibody-colloidal gold marker; and the nitrocellulose membrane is coated with diazepam coupling antigens and Goat anti-Rb IgG anti bodies; wherein, the diazepam coupling antigen is taken as a test line and the Goat anti-Rb IgG anti body is taken as a quality control line. The test paper provided by the invention is economical and rapid and can be used conveniently and made easily atlow cost.

Description

technical field [0001] A diazepam colloidal gold method detection test paper and a preparation method thereof belong to the technical field of biological immune method determination. Background technique [0002] Diazepam is a benzodiazepine sedative and hypnotics, chemical name: 1-methyl-5-phenyl-7-chloro-1,3-dihydro-2H-1,4-benzodiazepine Zol-2-one. Used as an animal feed additive, as a growth promoter, it can make animals sleepy and less active, grow faster and change the meat quality. It is also used for sedation before slaughtering and animal quarantine, or before transferring animals to slaughter, so as to reduce animal tension and reduce animal injury and mortality. Drowsiness, weakness, headache, dizziness, nausea, constipation, and occasional rash, liver damage, bone marrow suppression and other adverse reactions can be seen with these drugs. If such drugs are randomly added to the feed, the accumulated drugs will enter the human body through the food chain, which...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/52G01N33/558
Inventor 胥传来李秋生刘丽强袁缓彭池方胡拥明
Owner JIANGNAN UNIV