Novel enzymes

An endoglucanase and sequence technology, applied in the directions of enzymes, hydrolases, glycosylases, etc., can solve the problems of reducing the strength of fabrics and increasing the burden of washing equipment.

Active Publication Date: 2009-01-14
AB ENZYMES GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional stone washing using pumice reduces the strength of fabrics and increases the burden on washing equipment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0121] Embodiment 1, the cultivation of acremonium thermophile ALKO4245

[0122] Acremonium thermophila strain ALKO4245 in a 2 liter bioreactor (Braun Biostat B, Braun, Melsungen, Germany) were grown in g / l in the following media: Solka Floc cellulose 40, corn extract powder 15, brewers' used grain 5, oat xylan 3, locust bean gum 3 , (NH 4 ) 2 SO 4 5 and KH 2 PO 4 5. The pH range is 5.2±0.2(NH 3 / H 2 SO 4 ), ventilation 1vvm, stirring 300-600rpm, with Struktol Defoaming control, temperature 42°C. The culture time is 4 days. After incubation, cells and other solids are collected by centrifugation, and the supernatant is recovered.

Embodiment 2

[0123] Example 2. Purification of endoglucanase from Acremonium thermophila ALKO4245

[0124] The culture supernatant of Acremonium thermophila ALKO4245 grown as described in Example 1 was incubated at 70° C. for 24 hours after concentration by ultrafiltration. Pure endoglucanase was obtained by sequential purification using hydrophobic interaction and cation exchange chromatography followed by gel filtration. The endoglucanase activity of the fractions collected during the purification was determined using carboxymethylcellulose (CMC) as substrate (according to the method of IUPAC, 1987).

[0125] The concentrated culture supernatant was added to a HiPrep 16 / 10 Butyl FF hydrophobic interaction column (GE Healthcare) containing 1M (NH 4 ) 2 SO 4 Equilibrate with 20 mM potassium phosphate buffer at pH 6.0. Bound protein was eluted with a linear gradient from the above buffer to 5 mM potassium phosphate buffer, pH 6.0. Fractions were collected and assayed for endoglucanase ...

Embodiment 3

[0132] Cloning of the cel45A and cel45B genes of embodiment 3, Acremonium thermophila (ALKO4245)

[0133] Standard molecular biology methods are used in the isolation and enzymatic treatment of DNA (plasmids, DNA fragments), transformation of E. coli, and the like. The basic methods used are described in standard molecular biology handbooks, eg Sambrook, J. et al., 1989 and Sambrook J. and Russell, D.W., 2001.

[0134] In Lambda DASH A genomic library of Acremonium thermophila ALKO4245 was constructed in II vector (Stratagene, USA) according to the manufacturer's instructions. Chromosomal DNA was isolated by the method of Raeder and Broda, 1985 and partially digested with Sau3A. Digested DNA was size fractionated on an agarose gel and fragments of selected size (approximately 5-23 kb) were isolated, dephosphorylated, and ligated into BamHI digested vector arms. The ligation mix was packaged using Gigapack III Gold pack extract according to the manufacturer's instructions (...

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Abstract

The present invention relates to novel cellulase enzymes, especially novel endoglucanases including endoglucanase fusion proteins, preparations and compositions containing these endoglucanase enzymes and fusion proteins, expression vectors, host cells and methods for their preparation and uses of the cellulases, preparations and compositions in the textile, detergent and pulp and paper industries.

Description

field of invention [0001] The present invention relates to novel cellulases, especially novel endoglucanases including endoglucanase fusion proteins, products and compositions containing these endoglucanases and fusion proteins, and materials for their preparation Expression vectors, host cells and methods, and uses of cellulases, preparations and compositions in the textile, detergent and pulp and paper industries. Background of the invention [0002] Cellulose is the main structural component of higher plants and exists only in almost pure form in cotton fibers under natural conditions. It provides high tensile strength to plant cells, helping them resist mechanical stress and osmotic pressure. Cellulose is a linear polysaccharide with glucose residues linked by β-1,4 bonds. Under natural conditions, cellulose is usually linked together with lignin and hemicelluloses, such as xylan and glucomannan. Cellulolytic enzymes hydrolyze cellulose, produced by a wide variety of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12N15/62C12N15/56D06M16/00C11D3/386D21C5/00A23K1/165A23K10/14
CPCC11D3/38645C12N9/2437C12N9/244C12N15/62C12N15/79C12Y302/01004D06M16/003D06P5/02D21C5/005D21H17/005
Inventor 莱纳·瓦尔塔卡里玛丽卡·阿拉普拉内恩萨图·胡曼马蒂·西伊卡-阿霍亚尔诺·卡利奥利萨·维卡里彭蒂·奥亚帕洛亚里·韦赫曼佩雷
Owner AB ENZYMES GMBH
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