Double enzyme coupling preparation of ornithine hydrochloride

A technology of ornithine hydrochloride and hydrochloric acid, which is applied in the field of preparation of ornithine hydrochloride, can solve the problems of high cost, great environmental damage, unguaranteed product yield and purity, and achieve low cost and environmental protection. Low pollution and low production cost

Inactive Publication Date: 2011-01-12
NINGBO ZHENHAI HAIDE BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the current production methods rely too much on chemical reagents, causing great damage to the environment, high cost, and product yield and purity cannot be guaranteed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation of arginase:

[0032] Take 100g of fresh bovine liver, pulverize it in a freezer, break the cell wall, and then store it at 4°C for later use. Take 100g of bovine liver slurry and dissolve it in 400ml deionized water, add a catalyst, the catalyst is manganese sulfate, and the effective catalytic component is mn 2+ ions, and the concentration was controlled at 0.2mmol / L, heated to 30°C, kept warm for 0.5 hours, filtered, and the filtrate was collected. After high-speed centrifugation, the filtrate was immediately stored in a 4°C refrigerator for use.

[0033] Preparation of urease:

[0034] Take 100g of fresh soybeans, crush them, mix them with 30% ethanol by volume, the mass percentage of soybeans and ethanol is 1:3, then shake at 10°C for 10 minutes, take the supernatant after high-speed centrifugation, use at 30°C, and keep warm for 40 minutes, and stored below 4°C.

[0035] Conversion reaction:

[0036] Dissolve 100g of dry arginine in 2000ml of deion...

Embodiment 2

[0040] Preparation of arginase:

[0041] Take 100g of fresh beef liver, grind it in a freezer, break the cell wall, and then store it at 4°C for later use. Take 100g of beef liver slurry and dissolve it in 100ml of deionized water, add a catalyst, the catalyst is manganese acetate, and the effective catalytic component is mn 2+ ions, and the concentration was controlled at 0.6mmol / L, heated to 70°C, kept for 2 hours, filtered, and the filtrate was collected. After high-speed centrifugation, the filtrate was immediately stored in a 4°C refrigerator for use.

[0042] Preparation of urease:

[0043]Take 100g of fresh soybeans, crush them, mix them with 30% ethanol by volume, the mass percentage of soybeans and ethanol is 1:2, then shake at 0°C for 10 minutes, take the supernatant after high-speed centrifugation, use at 35°C, and keep warm for 70 minutes, and stored below 4°C.

[0044] Conversion reaction:

[0045] Dissolve 100g of dry arginine in 300ml of deionized water, adj...

Embodiment 3

[0049] Preparation of arginase:

[0050] Take 100g of fresh beef liver, grind it in a freezer, break the cell wall, and then store it at 4°C for later use. Take 100g of beef liver slurry and dissolve it in 200ml of deionized water, add a catalyst, the catalyst is manganese chloride, the effective catalytic component for Mn 2+ ions, and the concentration was controlled at 0.4mmol / L, heated to 35°C, kept warm for 1 hour, filtered and collected the filtrate, and the filtrate was centrifuged at a high speed, and immediately stored in a 4°C refrigerator for use.

[0051] Preparation of urease:

[0052] Take 100g of fresh soybeans, crush them, mix them with 30% ethanol by volume, the mass percentage of soybeans and ethanol is 1:2.9, then shake at 2°C for 10 minutes, take the supernatant after high-speed centrifugation, use at 35°C, and keep warm for 60 minutes, and stored below 4°C.

[0053] Conversion reaction:

[0054] Dissolve 100g of dry arginine in 1800ml of deionized water...

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PUM

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Abstract

The invention relates to a method for preparing ornithine hydrochloride, in particular to a double-enzyme coupling method for preparing the ornithine hydrochloride. The invention provides an economic and environment-friendly double-enzyme coupling method for preparing the ornithine hydrochloride. The method fully utilizes biological enzymes to prepare the product of the ornithine hydrochloride and is distinguished from the most production methods which depend on chemical reagent too much in the prior art. The method adopts urease extracted from fresh soybeans and bovine liver enzyme solution extracted from fresh bovine livers to prepare the ornithine hydrochloride.

Description

technical field [0001] The invention relates to a preparation method of ornithine hydrochloride, in particular to a double-enzyme coupling preparation method of ornithine hydrochloride. Background technique [0002] Ornithine, an amino acid, is produced by the metabolism of arginine (Arginine) to produce urea (the composition of urine). It is an important pharmaceutical intermediate, raw material drug and food additive, mainly used for treatment Cirrhosis of the liver, recovery from disease, and enhancement of physical fitness. [0003] Domestic publication number is CN1590367 a kind of preparation method of L-ornithine hydrochloride, and its main content comprises: L-arginine is dissolved in water, adds phase-transfer catalyst crown ether and milk of lime calcium hydroxide, stirs and refluxes After heating, add cocatalyst choline, and then adjust the pH value with 6N sulfuric acid after the reaction mixture is cooled. Calcium sulfate is removed by filtration, and the filt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/00C12N9/78C12N9/86
Inventor 王雷孙军缪铭周玉平张姝张勇吴培立黎法国潘庆伟
Owner NINGBO ZHENHAI HAIDE BIOCHEM
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