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Carbon shell bacterium polyketone, preparation and uses thereof

A technology of charcoal shell bacteria and polyketone, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, medical preparations containing active ingredients, etc., to achieve the effects of simple process, low cost and guaranteed source

Inactive Publication Date: 2009-02-18
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there has been no report on the isolation of immunosuppressants from the insect gut bacterium Daldinia eschscholzii

Method used

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  • Carbon shell bacterium polyketone, preparation and uses thereof
  • Carbon shell bacterium polyketone, preparation and uses thereof
  • Carbon shell bacterium polyketone, preparation and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Isolation and Purification of Insect Gut Fungus Daldinia eschscholzii

[0030] Dissect Tenodera aridifolia under aseptic conditions, disinfect the intestines of the removed mantis in 75% alcohol for 2 minutes, rinse with sterile water for 3 times, add a small amount of sterile water, and grind in a sterile mortar. Gradiently dilute the polishing solution with sterile water to 10 -1 , 10 -2 , 10 -3 , 10 -4 , take 0.2 ml of each gradient dilution solution and spread them on MEA medium (20 g of malt extract, 20 g of sucrose, 1 g of peptone, 15 g of agar, 1 L of distilled water), and place them in a 28°C incubator for cultivation. , picking and purifying the colony edge hyphae to obtain insect intestinal fungus, which was identified as Daldinia eschscholzii TL01 by the Guangdong Provincial Institute of Microbiology, and is now preserved in the China Type Culture Collection Center (Address: Wuhan Luo Jiashan Wuhan University), the preservation number is CCTCC ...

Embodiment 2

[0031] Embodiment 2: the liquid fermentation of insect gut fungus Daldinia eschscholzii

[0032] Activate insect intestinal fungus Daldinia eschscholzii, inoculate fresh mycelium blocks into 1000mL Erlenmeyer flasks, each bottle contains 400mL of ME medium, inoculate 5-6 bottles on a shaker, Cultivate at 140rpm and 28°C for 3 days as seed liquid, then inoculate 20mL of seed liquid into a 1000mL Erlenmeyer flask containing 400mL of ME medium, and ferment at 140rpm and 28°C for 10 days.

Embodiment 3

[0033] Embodiment 3: the extraction and separation of carbon shell fungus polyketide (dalesconols)

[0034] The fermented liquid obtained in Example 2 was filtered through gauze, the filtrate was extracted with ethyl acetate, concentrated and dried in vacuo to obtain black crude extract F1. Suspend the crude extract F1 in water, sequentially extract with petroleum ether and ethyl acetate, and concentrate the obtained ethyl acetate extract to obtain extract F2. Perform silica gel column chromatography on extract F2, and wash with 10 times the volume of chloroform first. The red part eluate was combined to obtain red extract F3, and then eluted with a mixed solution of chloroform / methanol (100 / 1 by volume), and the red part eluate was combined to obtain red extract F4. For the extract F3, recrystallize with methanol to obtain racemic carbonaceous polyketone A (dalesconol A), and use chiral high-pressure liquid chromatography (chiral column: ChiralpakAS-H column; mobile phase: n-...

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PUM

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Abstract

The invention discloses novel skeleton dalesconols, which has a general formula described at the right; wherein, 1,3,5,7,9,11,13,15,17,18,19,21,23,25,27 and 29 are the numbers of carbon atomic ordinal, R<1> is H or OH, when R<1> is H, dalesconols is dalesconols A; and when R<1> is OH, dalesconols is dalesconols B. The dalesconols has strong competitive inhibition to the mouse spleen cell proliferation caused by Con A, and has no toxicity to the normal mouse spleen cells; thus the dalesconols can be used as the compound with the functions of immunity and inhibition, and also can be applied to the preparation of the relevant novel medicine.

Description

technical field [0001] The invention relates to a new class of dalesconols extracted from Tenodera aridifolia intestinal bacterium Daldinia eschscholzii liquid fermentation product, its preparation method and immunosuppressive effect. Background technique [0002] Although the secondary metabolites of microorganisms have been widely studied, the metabolites of some special environment microorganisms have not attracted enough attention. Insect gut bacteria are one of the special environment microorganisms, and there are few studies on their metabolites. It is estimated that there are about 30 million species of insects on the earth, and the diversity of insects means the diversity of their gut bacteria, which provides a rich source of secondary metabolites. [0003] Immunosuppressants play an important role in organ transplantation and autoimmune diseases. It is reported that some insect fungi can produce some secondary metabolites to inhibit the immune system of insects af...

Claims

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Application Information

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IPC IPC(8): C07C49/513C12P7/26A61K31/122A61P37/06C12R1/645
Inventor 谭仁祥徐强张应烙赵蔚黎书华
Owner NANJING UNIV
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