Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Corn bud with high activity of saponin glycosidase and use thereof

A technology of glucoside glycosidase and grain sprouts, which is applied in the field of grain sprouts, can solve the problems of low hydrolysis ability of saponins and glycosides, and achieve the effects of strong hydrolysis ability, simple production process and low cost

Active Publication Date: 2009-03-18
SUNFLOWER PHARM GRP (TIANJIN) DRUG RES INST CO LTD
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the enzymes of the existing grain buds have low hydrolysis ability to the main active components of Chinese herbal medicines - saponins and glycosides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Wash 1 kg of barley with water, soak in germination water at 16°C for 4 to 8 hours, and after germination for 12 to 16 hours, use 50 ml of commercially available gibberellic acid and 15 mg of ginsenosides in an aqueous solution or buffer solution (acetic acid buffer, phosphate buffer or tris buffer) sprayed and mixed, germinated for 4 to 5 days at 16°C, and dried (drying method, edited by Guan Dunyi: Handbook of Beer Industry, China Light Industry Press, 1998; p99- 120), that is, to make malt with high ginsenoside glucosidase activity.

[0012] 500 grams of the above-mentioned malts (calculated by dry weight) were mashed, added to 3000 milliliters of 0.02 mole, pH 5.0 acetate buffer and soaked for one hour, centrifuged to remove impurities, the supernatant was added ammonium sulfate with a saturation of 66%, and the precipitate was collected by centrifugation. Dialyze with 0.02 molar, pH 5.0 acetic acid buffer solution to remove salt, then use the buffer solution to fix...

Embodiment 2

[0018] Wash 1 kg of barley with water, soak in germination water at 16°C for 4 to 8 hours, and after germination for 12 to 16 hours, use 50 ml of gibberellic acid and 8 mg of Pulsatilla saponin (according to the literature: "Liu Lin etc., Journal of Dalian Institute of Light Industry, 23 (1), 18-21, 2000 "method extraction) aqueous solution or buffer solution (acetate buffer, phosphate buffer or tris buffer) solution spray mixing, 16 ℃ to continue to germinate 4 ~ After 5 days, dry (drying method, edited by Guan Dunyi: Handbook of Beer Industry, China Light Industry Press, 1998; p99-120), that is, malt with high Pulsatilla saponin glycosidase activity is made.

[0019] Extract enzyme and process Pulsatilla saponin according to the method of Example 1; Its reactant is measured with thin-layer chromatography (TLC) and high-performance liquid chromatography (Wang Wei: Journal of Dalian Institute of Light Industry, 24 (1), 4-7, 2005) Results: 80% of Pulsatilla saponins with 5 suga...

Embodiment 3

[0022] Wash 1 kg of barley with water, immerse in germination water at 16°C for 4 to 8 hours, and then germinate for 12 to 16 hours. solution, phosphate buffer or tris buffer), spray and mix, continue to germinate at 16°C for 4 to 5 days, and dry (drying method, edited by Guan Dunyi: Handbook of Beer Industry, China Light Industry Press, 1998; p99-120), ready-made into malt with high rutinase activity.

[0023] Extract enzyme and process rutin according to embodiment 1,2 method; Its reactant uses thin-layer chromatography (TLC) and high-performance liquid chromatography (Wei Shenghua: Journal of Dalian Institute of Light Industry, 22 (2), 114-116, 2003) Determination results: 85% of rutin with 2 sugar groups was degraded into isoquercetin and quercetin with 1 sugar group.

[0024] The rutin added in the above malt preparation process is replaced by Epimedium or soybean isoflavone glycosides (glycosides) to prepare malt, and the prepared malt is used to enzymatically hydrolyze...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses a grain bud with the high activity of saponin or glycoside glycosidase and an application thereof. The grain bud with the high activity of the saponin or the glycoside glycosidase is prepared by a method as follows: gibberellin and ginseng saponin or windflower saponin or yam saponin or astragalus saponin or icariine or isoflavone glycoside or rutin glycoside are added during the process of preparing the grain bud including barley, broomcorn, corn and wheat bud, to ensure that the grain bud germinates and is dried to respectively obtain the corresponding grain bud with the high activity of the saponin or the glycoside glycosidase. The present invention has simple making technology and low cost, has high hydrolyzing capacity to the main active components of the saponin and glycoside glycosyl in Chinese herbal medicines and can be used for hydrolyzing the ginseng saponin or windflower saponin or yam saponin or astragalus saponin or icariine or isoflavone glycoside or rutin glycoside to prepare the corresponding secondary low-glycosyl saponin or glycoside with high activity.

Description

Technical field: [0001] The present invention relates to a kind of grain bud and its application, especially a kind of saponin or saponin which has simple production process and low cost and can be used for enzymatic hydrolysis of saponin or glycoside to prepare secondary saponin, glycoside or aglycone with higher activity. Grain sprouts with high glucosidase activity. Background technique: [0002] The main active ingredients of Chinese herbal medicine—saponin (Saponin) and glycoside (Glycoside) are not the most active structures. After Chinese herbal medicine is taken orally, it needs to undergo the action of enzymes in the digestive system and intestinal microorganisms to change saponins and glucosides into highly active low-sugar-based components, which are absorbed by the intestines and enter the blood, and then exert their medicinal effects (M Ishihara: Yakugaku Zasshi, 122( 9), 695-701, 2002). However, the change of saponins and glycosides in the body is very weak, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A23L1/186A23L1/29A23L1/30A23L33/105
Inventor 金凤燮鱼红闪
Owner SUNFLOWER PHARM GRP (TIANJIN) DRUG RES INST CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com