Method for preparing bacteria cellulose compound film and application thereof as face pack material

A technology of bacterial cellulose film and bacterial cellulose, which is applied in the application field of bacterial cellulose composite film as a mask material, can solve the problems of inconvenient use, the performance of the mask being affected by a specific carrier, and the poor ductility of the mask, etc. Water content, low cost, and the effect of improving conversion rate

Inactive Publication Date: 2009-03-18
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it must be configured according to certain requirements before use, which is inconvenient to use.
In addition, the mainstream masks on the market are mainly paper masks, which have obvious disadvantages, such as the poor ductility of the mask, and the performance of the mask is affected by specific

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Strain selection: Gluconacetobacter xylinum CGMCC No.1.1812 was selected.

[0034] (2) Preparation of polyglutamic acid containing seeds and liquid medium: in weight percentage, containing 2% fructose, 0.5% yeast powder, 0.5% tryptone, 0.5% Na 2 HPO 4 , 0.2% citric acid, 2% calcium carbonate seed medium or liquid medium, add 0.5g / L polyglutamic acid, then adjust the pH value of the medium to 6, and sterilize at a temperature of 121°C for 20min, After cooling to 30°C, set aside;

[0035] (3) Seed cell culture: Take the slant seeds (Gluconacetobacterxylinum (Gluconacetobacterxylinum) CGMCC No.1.1812) that have been activated in the 1-2 rings and insert them into the liquid medium containing polyglutamic acid, shake and cultivate at 30°C for 24 hours, shake The bed speed was 160r / min to obtain the seed liquid;

[0036] (4) Production of bacterial cellulose by static liquid fermentation: Inoculate the seed liquid into the liquid medium containing polyglutamic acid with...

Embodiment 2

[0040] Calculated by weight percentage, take 2% glucose, 0.5% yeast powder, 0.5% tryptone, 0.5% Na 2 HPO 4 , 0.2% citric acid, 2% calcium carbonate, 0.05% polyglutamic acid, adjust the pH to 6.0. Sterilize at 121°C for 20min. Using this as a liquid medium, take a ring of activated slant seed Acetobacter xylinum (Gluconacetobacter xylinum) CGMCC No.1.2378, insert it into the liquid medium, and cultivate it with shaking at 30°C for 24 hours, with the shaker speed at 160r / min, as seed liquid. Inoculate the liquid culture medium (500mL Erlenmeyer flask contains 200mL liquid medium) with 6% inoculum amount, fully shake it when inoculating, so that the bacteria liquid is uniform, and culture at a constant temperature of 30°C for 6 days. The bacterial cellulose film generated after standing at constant temperature for 6 days floated on the liquid surface. After the membrane is taken out, rinse with water several times to remove the culture medium and impurities on the surface of t...

Embodiment 3

[0043] Calculated by weight percentage, take 2% glucose, 0.5% yeast powder, 0.5% tryptone, 0.5% Na 2 HPO 4 , 0.2% citric acid, 2% calcium carbonate, 0.04% polyglutamic acid, adjust the pH to 6.0. Sterilize at 121°C for 20min. Using this as a liquid medium, take a ring of activated slant seeds (Gluconacetobacter xylinum) CGMCC No.1.1812, insert it into the liquid medium, and cultivate it with shaking at 32°C for 24 hours, with the shaker speed at 160r / min, as the seed . Inoculate 7% of the inoculum into the liquid culture medium (distribute into shallow fermentation trays, clean the fermentation trays, and sterilize with ozone water), fully shake when inoculating to make the bacterial liquid uniform, with a thickness of 10mm, at 32°C Constant temperature static culture for 6 days. The bacterial cellulose film generated after standing at constant temperature for 6 days floats on the liquid surface with a thickness of about 8 mm. After the membrane is taken out, rinse with wa...

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PUM

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Abstract

The invention discloses a preparation method for a bacterial cellulose composite membrane and application of the bacterial cellulose composite membrane as a facial mask material. The preparation method comprises the following steps: (1) selecting a strain; (2) preparing a seed containing a polyglutamic acid (PGA) and a liquid culture medium; (3) culturing seed cells; (4) producing bacterial cellulose by static liquid fermentation; and (5) purifying a bacterial cellulose membrane. The bacterial cellulose composite membrane can be used as a facial mask carrier or is directly used as a wet facial mask. The preparation method improves the yield of the bacterial cellulose by adding the polyglutamic acid (PGA) into the culture medium, improves the water content and the mechanical strength of a wet membrane, and has simple process and low cost; and the experiments prove that the method can ensure that the dry weight of the bacterial cellulose is improved to between 14 and 16g/L from the prior dry weight of between 8 and 10g/L, and the water content is increased to 99 percent from 98 percent.

Description

technical field [0001] The invention relates to a preparation method of a bacterial cellulose composite film and the application of the bacterial cellulose composite film as a mask material. Background technique [0002] Bacterial cellulose was discovered by Brown in 1886. Acetobacter xylinum formed a layer of white fibrous substance on the surface of the medium during static culture. It was determined by chemical and physical analysis that this substance has the structure and structure of cellulose. Chemical properties, named bacterial cellulose (bacterial cellulose BC) because it is synthesized by bacteria. The diameter of bacterial cellulose is only 1 / 10 of that of artificial synthetic fibers, ranging from 10nm to 100nm. It is a new type of nano-scale biological material. It has many unique properties, such as high crystallinity and high chemical purity, high tensile strength and elastic modulus, strong water binding, excellent shape retention and tear resistance, high b...

Claims

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Application Information

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IPC IPC(8): C12P19/04A61K8/73A61Q19/00C12R1/02
Inventor 马霞
Owner SHANGHAI INST OF TECH
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