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Breeding method of microbial feed additive strain

A microbial feed and additive technology, applied in the fields of microbiology, animal nutrition and feed science, can solve the problems of few reports

Inactive Publication Date: 2009-03-25
BEIJING DABEINONG TECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on probiotic strains at home and abroad is mostly focused on the development of products and application effects. According to the specific requirements of probiotics, strains with excellent performance are selected, and there are not many reports on key indicators such as the biological characteristics of strains; in the field of animal feed However, there are fewer research reports on systematic breeding methods for strains with excellent performance at home and abroad, and most of the existing reports on breeding methods have their limitations, and the screening standards and specific test methods for animal probiotic strains have not been organically integrated. combined to form a set of systematic, perfect, and practically operable microbial feed additive strain breeding methods, which is also the innovation of the present invention

Method used

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  • Breeding method of microbial feed additive strain
  • Breeding method of microbial feed additive strain
  • Breeding method of microbial feed additive strain

Examples

Experimental program
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Effect test

Embodiment 1

[0038] (1) Isolation and purification of spore-like bacteria

[0039] Bacterial enrichment culture: Inoculate a little content from the small intestine, large intestine and cecum of piglets near the mucosa into BPY liquid medium, and carry out aerobic bacterial enrichment culture in a constant temperature culture shaker at 37°C;

[0040] Separation and purification: Boil the above-mentioned enriched bacterial solution in boiling water for 10 minutes, and then streak culture on the BPY medium plate, from which various colonies with different shapes are obtained, streak culture and separation, and microscopically check whether there is spore formation , Whether it is a single colony or not, inoculate the 32 isolated pure colonies and bacillus bacteria into the slant of the BPY medium test tube to proliferate, and then store them in a refrigerator at 4°C until use.

[0041] The composition of BPY medium is as follows: beef extract 0.3-0.5g, yeast extract 0.3-0.5g, peptone 0.5-1g,...

Embodiment 2

[0048] A total of 72 bacterial strains of embodiment 1 are screened for the excellent bacterial strains resistant to digestive tract habitat

[0049] (1) The preserved slant strains were inoculated in a test tube containing 10 mL of liquid medium, cultured at 37°C for 20-24 hours, and then inoculated into 9.0 mL of artificial simulated gastric juice with a pH value of 2.0, 3.0, and 4.0 according to 10% of the inoculation amount. Count at 0h as a control, sample at 2h and 6h with 10-fold serial dilution with phosphate buffer, count the viable bacteria on the plate, calculate the survival rate, and screen the excellent strains with a survival rate of more than 1%.

[0050] Preparation of artificially simulated gastric juice: measure 16.4 ml of 9.5%-10.5% concentrated hydrochloric acid, add distilled water to 1000 ml, make basic artificial gastric juice, adjust pH value to 2.0, 3.0, 4.0 with hydrochloric acid or sodium hydroxide, take 10 mL (9 mL) each , Distributed into test tub...

Embodiment 3

[0055] The 7 bacterial strains screened in Example 2 carry out the analysis and determination of metabolites and the screening of excellent strains with good probiotic properties

[0056] Determination of acid production: use ion chromatography to analyze and measure the content of organic acids in the fermentation broth, and screen excellent strains that produce a certain amount of organic acids such as lactic acid, butyric acid, acetic acid, and citric acid. The results show that the three strains of lactic acid bacteria DW-SGY-49, DW- The lactic acid yields of BLKY-27 and DW-CMJ-47 reached 9.132g / L, 4.141g / L, and 3.758g / L respectively, and the percentages of lactic acid in the total acid yield were 95%, 85.5%, and 89.4% respectively; 4 strains of Bacillus DW - The organic acid yields of USA / BT-10, DW-RDYL-46, DW-AQFB-1, and DW-ZKY-55 were 1.230, 0.298, 1.814, and 1.695 g / L, respectively.

[0057] Determination of antibacterial effect: The antibacterial effect of the strains...

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Abstract

The invention pertains to the fields of microbiology and science of animal nutrition and feedstuff and relates to a breeding method of microbial feedstuff additive strains, which comprises: separation and purification of strains, seed selection of stress resistance, seed selection of probiotic functionality, identification of strains and / or safety research. The method is characterized by scientificalness, reasonableness, complete system and strong operation. By the adoption of the method, new products of the microbial feedstuff additive strains with excellent resistance, strong function and outstanding efficiency can be cultured, thus providing guarantee of important strain resources for the development of microbial feedstuff additive products.

Description

technical field [0001] The invention relates to a breeding method for microbial feed additive strains, and belongs to the fields of microbiology, animal nutrition and feed science. Background technique [0002] Feed additives such as antibiotics have played a huge role in preventing animal diseases, improving animal production performance and economic benefits, but at the same time, they have also caused serious negative effects, such as drug resistance of pathogenic microorganisms, decreased animal immunity, and drug residues. Human health damage and excrement polluting the environment, etc. With the improvement of living standards, people pay more and more attention to the quality of consumer goods, and the safety of animal products has increasingly become the focus of attention. At present, the European Union has banned the use of antibiotics. In recent years, every year in my country, livestock products have been returned or destroyed due to excessive drug residues, cau...

Claims

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Application Information

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IPC IPC(8): C12N1/00A23K1/16A23K10/18
Inventor 孙占敏赵雁青莫云宋维平张玳华姚琨贾秋英白玉卿刘宇赵军
Owner BEIJING DABEINONG TECH GRP CO LTD
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