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Rapid detection method and kit for melamine adulteration in food or feed

A melamine and detection method technology, which is applied in the preparation of test samples, measuring devices, material separation and other directions, can solve the problems of difficulty in promotion for small and medium-sized enterprises, high inspection costs, and long inspection periods, and achieves easy promotion, easy mastery, and operation. easy effect

Inactive Publication Date: 2011-12-14
广东省疾病预防控制中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On September 11, 2008, relevant departments disclosed that some milk powder contained melamine, which caused some infants to develop kidney stone symptoms due to consumption of melamine-containing milk powder, and a small number of infants died of ineffective rescue.
[0008] Existing inspection methods (high performance liquid chromatography, liquid-mass spectrometry and gas-mass spectrometry) have high equipment investment costs, long inspection cycles, high inspection costs, and inspection operations are not easy for ordinary staff to master. , Small and medium-sized enterprises are difficult to promote and other shortcomings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: the detection of liquid milk (positive)

[0038] Take 12mL of liquid milk with a known melamine content of 500mg / L, put it in a 25mL colorimetric tube, add 6mL of methanol, add 1mL of trichloroacetic acid solution with a concentration of 10% by mass, and add 1mL of water (to make the amount of methanol in the mixed sample The volume percent concentration is about 30%), homogenize on a vortex oscillator for 2 minutes, sonicate for 10 minutes, centrifuge for 5 minutes (3000r / min), absorb about 8mL of the supernatant, inject it into the activated solid phase extraction column, and the initial outflow About 1mL of the solution was discarded, and about 3-5mL of the solution that flowed out was collected with a test tube as the test solution. Add 10 drops (about 0.5 mL) of a saturated solution of trinitrophenol into the test solution, and let stand without shaking. Needle-like crystals can be observed in the test tube after standing still. It was judged that t...

Embodiment 2

[0040] Embodiment 2: the detection of liquid milk (negative)

[0041] Take 12mL of liquid milk known not to contain melamine, put it in a 25mL colorimetric tube, and refer to Example 1 for the rest of the operations. After standing still, the solution in the test tube was clear without precipitates. It is thus judged that the sample does not contain higher levels of melamine.

Embodiment 3

[0042] Embodiment 3: the detection of milk powder (positive)

[0043] Take 5g of milk powder whose melamine content is known to be 500mg / L, put it in a 50mL colorimetric tube, add about 35mL of a 30% methanol solution by volume, add 2.5mL of 10% trichloroacetic acid, and then use the volume percent The methanol solution with a concentration of 30% was adjusted to the scale, homogenized on a vortex oscillator for 2 minutes, ultrasonicated for 10 minutes, centrifuged for 5 minutes (3000r / min), and about 8 mL of the supernatant was absorbed, injected into the activated solid phase extraction column, and the original About 1mL of the solution that flowed out was discarded, and about 3-5mL of the solution that flowed out was collected with a small test tube as the test solution. Add 10 drops (about 0.5 mL) of a saturated solution of trinitrophenol into the test solution, and let stand without shaking. Needle-like crystals can be observed in the test tube after standing still. It ...

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PUM

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Abstract

The invention discloses a rapid detection method for melamine doping in food or feed and a kit thereof. Including the following steps: (1) remove most of the protein in the sample to be tested; (2) remove the trace protein remaining in the supernatant to prepare the test solution; (3) crystallization reaction and result judgment: add to the test solution Saturated aqueous solution of trinitrophenol, stand still; When the test solution contains melamine, add the saturated aqueous solution of trinitrophenol, after standing, needle-like crystals will be observed in the test tube; test solution without melamine No precipitates. The kit includes the following reagents: methanol, 10% by mass percent trichloroacetic acid solution, and saturated aqueous solution of trinitrophenol. The melamine detection method of the present invention has low cost, simple and convenient operation, saves time, does not use expensive instruments, is easy to be mastered by ordinary workers, and is easy to popularize.

Description

technical field [0001] The invention relates to a rapid detection technology and a kit for melamine. Background technique [0002] Melamine, also known as melamine, molecular formula: C 3 N 6 h 6 , The nitrogen content in the molecule is as high as 66.7%. Melamine is a chemical raw material that can be synthesized from urea. It is a raw material for the production of melamine-formaldehyde resin and is widely used. But melamine is not a food additive. [0003] The content of protein in food and feed is usually determined by "Kjeldahl method". The method is to digest the protein to generate ammonium salt, calculate the nitrogen content by measuring the ammonia content, and then calculate the protein content in the sample through the nitrogen content. The current product quality standards generally stipulate the minimum content requirements for food or feed materials with high protein content. For example, the protein content in fresh milk should be greater than or equal ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/00G01N30/06G01N1/28
Inventor 朱炳辉高燕红钟志雄龙朝阳杨杏芬张永慧
Owner 广东省疾病预防控制中心
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